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pHL1355

Plasmid

#37566

Depositor

Han Lim

Article

Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110

Inserts

GFP
TetR
CsrA

Use

Synthetic Biology

Tags

glgC leader region

Expression

Bacterial

Mutation

start codon removed

Promoter

pConNoHind and pConNoHindM12

Available Since

July 26, 2012

Availability

Academic Institutions and Nonprofits only
CB6-GFP-TAOK2

Plasmid

#197110

Purpose

Expression of GFP-tagged TAOK2 / PSK1 in mammalian cells

Depositor

Jonathan Morris

Article

Garg et al J Cell Sci. 2020 Mar 16;133(6):jcs235895

Insert

TAOK2 (TAOK2 Human)

Tags

GFP

Expression

Mammalian

Mutation

Wild Type

Promoter

CMV

Available Since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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EGFR-G574V-ET1

Plasmid

#253821

Purpose

Expression of full length EGFR with G574V mutation

Depositor

Timothy Springer

Article

Watson et al J Biol Chem. 2025 Jul;301(7):110374. doi

Insert

EGFR with G574V mutation (EGFR Human)

Use

Pires2-egfp

Expression

Mammalian

Mutation

G574V

Available Since

April 1, 2026

Availability

Academic Institutions and Nonprofits only

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EGFR-R84K-ET1

Plasmid

#253818

Purpose

Expression of full length EGFR with R84K mutation

Depositor

Timothy Springer

Article

Watson et al J Biol Chem. 2025 Jul;301(7):110374. doi

Insert

EGFR with R84K mutation (EGFR Human)

Use

Pires2-egfp

Expression

Mammalian

Mutation

R84K

Available Since

April 1, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti-PE2max-BSD

Plasmid

#191102

Purpose

Lentiviral expression plasmid of PEmax prime editor with P2A-BSD marker

Depositor

Hyongbum Kim

Article

Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

Insert

PEmax-P2A-BSD

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

Changed R221K, N394K, H840A from SpCas9

Promoter

EF-1a

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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BirA-Myc_N_MYC

Plasmid

#167715

Purpose

Expresses MYC in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

MYC (MYC Human)

Tags

Myc-BirA

Expression

Mammalian

Mutation

no mutations

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-dLight1.2

Plasmid

#111054

Purpose

Expresses dLight1.2 in mammalian cells

Depositor

Lin Tian

Article

Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

Insert

dLight1.2

Tags

Flag tag

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV7.1_3xFLAG-MOB1A

Plasmid

#172988

Purpose

constitutive expression of FLAG-tagged MOB1A

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

3xFLAG-MOB1A (MOB1A Human)

Tags

3xFLAG

Expression

Mammalian

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV_18xTEAD_luc2

Plasmid

#173003

Purpose

firefly luciferase reporter vector with 18x clustered TEAD binding sites

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

18xTEAD-luc2

Use

AAV and Luciferase

Expression

Mammalian

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pAT2732-(PB-4_EF1α-Hygro_2A_rtTA3 [HA-BFP-KRAB-PUFa])

Plasmid

#251107

Purpose

Expression of KRAB-PUFa component of Casilio-i system

Depositor

Chia-Lin Wei

Article

Taghbalout et al Cancer Cell. 2025 Dec 8;43(12):2191-2205

Insert

BFP-KRAB-PUFa

Tags

HA

Expression

Mammalian

Mutation

N/A

Available Since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti_gRNA -hMLHdn-Puro

Plasmid

#191105

Purpose

Backbone for lentiviral pegRNA library construction with hMLH1dn and Puro marker

Depositor

Hyongbum Kim

Article

Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

Insert

hMLH1dn

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

I31 silent mutation (ATA to ATC) to remove early …

Promoter

EF-1a

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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TtEnd5A_pEHISTEV

Plasmid

#179732

Purpose

TtEnd5A_pEHISTEV expression vector encodes first Thermogutta terrifontis Endoglucanase (19-531 amino acid residues) of Glycoside Hydrolase Family 5.

Depositor

James Naismith

Article

Hussain et al Arch Biochem Biophys. 2025 Feb;764:11027

Insert

TtEnd5A

Tags

6xHis

Expression

Bacterial

Mutation

19 Amino acids from N-terminal encoding Signal pe…

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(cyto).iGlucoSnFR2

Plasmid

#244110

Purpose

Cytosolic expression of green glucose sensor

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2

Use

AAV

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pRK172-6xHis-0N4R-P301S-tau-HiBiT

Plasmid

#187835

Purpose

Expression of 6xHis-tagged P301S tau of 0N4R isoform with a C-terminal HiBiT tag for bimolecular luminescence with LgBiT.

Depositor

William McEwan

Article

Tuck et al Cell Rep. 2022 May 3;39(5):110776. doi:

Insert

6XHis-0N4R-P301S-tau-HiBiT

Tags

6xHistidine and HiBiT

Expression

Bacterial

Promoter

T7

Available Since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pHRm-NLS-dCas9-GFP11x7-NLS

Plasmid

#70224

Purpose

Expresses NLS-dCas9-GFP11x7-NLS in mammalian cells

Depositor

Bo Huang

Article

Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

Insert

NLS-dCas9-GFP11x7-NLS

Tags

GFP11x7

Expression

Mammalian

Available Since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-double floxed-Gat1-HA-WPRE-HGHpA

Plasmid

#184635

Purpose

Expresses the GABA membrane transporter Gat1 fused to HA, driven by the Ef1a promoter, in a Cre-dependent fashion

Depositor

Nicolas Tritsch

Article

Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

Insert

Gat1 (Slc6a1 Mouse)

Use

AAV

Promoter

EF1a

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-NRCH-PE2max-BSD

Plasmid

#191103

Purpose

Lentiviral expression plasmid of NRCH-PEmax prime editor with P2A-BSD marker

Depositor

Hyongbum Kim

Article

Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

Insert

NRCH-PEmax

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

Changed R221K, N394K, H840A from SpCas9-NRCH

Promoter

EF-1a

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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LZRS-HOTAIR

Plasmid

#26110

Depositor

Howard Chang

Article

Gupta et al Nature. 2010 Apr 15. 464(7291):1071-6.

Insert

HOTAIR (HOTAIR Human)

Use

Retroviral

Available Since

Sept. 15, 2010

Availability

Academic Institutions and Nonprofits only

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Halo-NEMO

Plasmid

#187899

Purpose

Mammalian cell expression of Halo-tagged NEMO

Depositor

Erika Holzbaur

Article

Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7

Insert

NEMO (Ikbkg Mouse)

Tags

Halo

Expression

Mammalian

Promoter

CMV

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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FR_HNF1A

Plasmid

#31104

Depositor

Gerhart Ryffel

Article

Senkel et al Biochim Biophys Acta. 2005 Dec 20. 1731(

Insert

HNF1A (HNF1A Human)

Use

Flp/frt

Tags

myc

Expression

Mammalian

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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