We narrowed to 3,171 results for: ALC

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pET28_6xHis-PARP1delZn2delBRCT

Plasmid

#173939

Purpose

Bacterial expression of PARP1 lacking Zn2 and BRCT domains

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1delZn2delBRCT (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Deletion of residues 97-213 and 374-520; V762A

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-E988K

Plasmid

#173945

Purpose

Bacterial expression of inactive PARP1 mutant (E988K attenuates catalytic and PAR-branching activity of PARP1)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-E988K (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

V762A, E988K

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-CATdeltaHD

Plasmid

#174811

Purpose

Bacterial expression of a hyperactive PARP1 CAT lacking the autoinhibitory HD subdomain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-CATdeltaHD (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Residues 678-787 replaced by eight-residue linker…

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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Control-cx43.4-48H-pGTag

Plasmid

#117818

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

TagRFP

Use

Synthetic Biology

Tags

CAAX

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pPRISM-gal4-VP16-gcry-TagBFP

Plasmid

#117777

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

gal4/VP16

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pNK7187

Plasmid

#219761

Purpose

MoClo-compatible Level 1 for expression PpASCL in plants (N.benthamiana, BY2 cells)

Depositor

Karen Sarkisyan

Article

Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

Insert

PpASCL

Expression

Plant

Promoter

p35s_0.4kb - 5'UTR TMV omega

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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BasiginCD4d3+4-Comp-His-Stop

Plasmid

#36147

Purpose

EXPRESs plasmid for human erythrocyte encoding BSG/EMMPRIN/CD147 and CD4d3+4-Comp-His-Stop pentamer

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

Basigin (BSG Human)

Tags

His tag, pentamerization domain of rat cartilagin…

Expression

Mammalian

Promoter

CMV

Available Since

May 14, 2012

Availability

Academic Institutions and Nonprofits only

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pPRISM-gal4-VP16-gcry-eGFP

Plasmid

#117779

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

gal4/VP16

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pPRISM-Stop-gcry-mRFP

Plasmid

#117772

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

3x3 Stop sequence

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pGTag-eGFP-B-actin

Plasmid

#117814

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

eGFP

Use

Synthetic Biology

Available Since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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pPRISM-gal4-VP16-cmlc2-mRFP

Plasmid

#117781

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

gal4/VP16

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-NDUFA5-mVenus

Plasmid

#185646

Purpose

NDUFA5 Complex I sub-unit with C-terminus mVenus tag

Depositor

Dipayan Chaudhuri

Article

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

NDUFA5 (NDUFA5 Human)

Tags

mVenus

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-CAT-L713F

Plasmid

#173944

Purpose

Bacterial expression of isolated PARP1 CAT domain containing L713F gain-of-function mutation

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-CAT-L713F (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

L713F, V762A

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pPRISM-Cre-cmlc2-eGFP

Plasmid

#117794

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

Cre

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pGTag-TagRFP-B-actin

Plasmid

#117808

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

TagRFP

Use

Synthetic Biology

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pOTTC810 - pAAV-EF1a-CRTsigpep-GCaMP3 (D324G+D360G+397G+D435G 10.19)-KDEL

Plasmid

#63885

Purpose

An AAV packaging vector that expresses ER-retained low-affinity GCaMP3 variant under control of the EF1a promoter.

Depositor

Brandon Harvey

Article

Henderson et al PLoS One. 2015 Oct 9;10(10):e0139273. do

Insert

ER-localized low-affinity GCaMP3(10.19)

Use

AAV

Promoter

EF1a

Available Since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pPRISM-TagRFP-gcry-eGFP

Plasmid

#117767

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

TagRFP

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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FOR human OCT4 TALEN

Plasmid

#52412

Purpose

TALEN expressing vector used to allow Knock-in of OCT4-GFP-2A-PURO knockin donor plasmid (generated by Jaenisch lab- Addgene Vector # 31939)

Depositor

Jacob Hanna

Article

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Insert

HD NG NN NN NN HD NG HD NG HD HD HD NI NG

Use

TALEN

Promoter

CAGGS

Available Since

June 6, 2014

Availability

Academic Institutions and Nonprofits only
pET28_6xHis-PARP1-L713F

Plasmid

#174794

Purpose

Bacterial expression of a hyperactive PARP1 mutant (L713F destabilizes the autoinhibitory HD subdomain)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-L713F (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

L713F, V762A

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-C125G

Plasmid

#174792

Purpose

Bacterial expression of a less active PARP1 mutant (C125G reduces affinity for single-strand DNA break)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-C125G (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

C125G, V762A

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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