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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5,038 results for: MLS

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Showing: 1941 - 1960 of 5038 results

  1. bla-A OR T-SpyTag

    Plasmid
    #246945
    Purpose
    Expresses multifunctional construct for OR-gated release of beta-lactamase (bla) from materials in response to sortase eSrtA(2A9) OR TEV treatment. Contains a SpyTag for material tethering.
    Depositor
    Cole DeForest
    Article
    Ross et al Angew Chem Int Ed Engl. 2025 Nov 10:e199
    Insert
    Please see depositor comments below
    Tags
    6x His tag
    Expression
    Bacterial
    Available Since
    Nov. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    246945-plasmid-map-sequence-id-489550

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  2. NanoLuc-A AND T-SpyTag

    Plasmid
    #246946
    Purpose
    Expresses multifunctional construct for AND-gated release of NanoLuciferase (NanoLuc) from materials in response to sortase eSrtA(2A9) AND TEV treatment. Contains a SpyTag for material tethering.
    Depositor
    Cole DeForest
    Article
    Ross et al Angew Chem Int Ed Engl. 2025 Nov 10:e199
    Insert
    Please see depositor comments below
    Tags
    6x His tag, SsrA
    Expression
    Bacterial
    Available Since
    Nov. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    246946-plasmid-map-sequence-id-489552

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  3. L4866 IL-10Rb NTEVp chain, IL-10Ra CTEVp chain with ZF1a in MGEV

    Plasmid
    #244190
    Purpose
    Multigene vector for constitutive expression of mTagBFP2, IL-10Rb NTEVp chain with human IgG VH signal peptide, and IL-10Ra CTEVp chain with ZF1a
    Depositor
    Joshua Leonard
    Article
    Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158
    Insert
    mTagBFP2; IL-10Rb NTEVp chain with human IgG VH signal sequence; IL-10Ra CTEVp chain with ZF1 synTF
    Use
    Synthetic Biology
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    NTEVp_75S, CTEVp_190K
    Promoter
    hEF1a, CMV
    Available Since
    Oct. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    244190-plasmid-map-sequence-id-489496

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  4. L4864 IL-10Rb NTEVp chain, IL-10Ra CTEVp chain with ZF6a in MGEV

    Plasmid
    #244189
    Purpose
    Multigene vector for constitutive expression of mTagBFP2, IL-10Rb NTEVp chain with human IgG VH signal peptide, and IL-10Ra CTEVp chain with ZF6a
    Depositor
    Joshua Leonard
    Article
    Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158
    Insert
    mTagBFP2; IL-10Rb NTEVp chain with human IgG VH signal sequence; IL-10Ra CTEVp chain with ZF6 synTF
    Use
    Synthetic Biology
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    NTEVp_75S, CTEVp_190K
    Promoter
    hEF1a, CMV
    Available Since
    Oct. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    244189-plasmid-map-sequence-id-489495

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  5. mCherry-S-SpyTag

    Plasmid
    #243786
    Purpose
    Expresses multifunctional construct enabling the YES-gated release of mCherry from materials in response to sortase eSrtA(4S9) treatment. Construct contains a SpyTag for material tethering.
    Depositor
    Cole DeForest
    Article
    Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/
    Insert
    mCherry
    Tags
    6x His tag
    Expression
    Bacterial
    Available Since
    Oct. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    243786-plasmid-map-sequence-id-486522

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  13. mGreenLantern-A AND S-SpyTag

    Plasmid
    #243775
    Purpose
    Expresses multifunctional construct enabling the AND-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9) AND sortase eSrtA(4S9) treatment. Contains a SpyTag for material tethering.
    Depositor
    Cole DeForest
    Article
    Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/
    Insert
    mGreenLantern
    Tags
    6x His tag and SsrA
    Expression
    Bacterial
    Available Since
    Oct. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    243775-plasmid-map-sequence-id-486143

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  16. L4342 TNFR1 CTEVp chain in PolyTX-mTagBFP2

    Plasmid
    #244178
    Purpose
    Constitutive expression of a MESA CTEVp chain encoding (N to C): TNFR1SS-3xFLAG-TNFR1ECD(dMMP)-TNFR1TMD-CTEVp(75S)-PRS(M)-VP64-ZF6
    Depositor
    Joshua Leonard
    Article
    Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158
    Insert
    MESA CTEVp chain with human TNFR1 SS, ECD (no MMP site) and TMD, CTEVp (190K), TEVp PRS (M in P1'), and synTF (VP64-ZF6) (TNFRSF1A Human, Synthetic)
    Use
    Synthetic Biology
    Tags
    TNFR1 signal peptide - 3xFLAG
    Expression
    Mammalian
    Mutation
    CTEVp_190K
    Promoter
    hEF1a
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    244178-plasmid-map-sequence-id-487910

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Showing: 1941 - 1960 of 5038 results