We narrowed to 11,102 results for: nar;

1. pAID1.2-EF1a-CmCherry-mAID

   Plasmid

   #140604

   Purpose

   Expression of OsTIR1 and mAID-mCherry protein in mammalian cells under the control of EF1a promoter

   Depositor

   Tatsuo Fukagawa

   Article

   Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

   Insert

   OsTIR1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJEC819

   Plasmid

   #190815

   Purpose

   CRISPRa backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.

   Depositor

   James Chappell

   Article

   Ameruoso et al Nucleic Acids Res. 2022 Jul 22;50(13):77

   Insert

   dCas9-XTEN-rpoA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   XTEN (linker), followed by the N-terminal domain …

   Expression

   Mutation

   Promoter

   SP30

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMS17

   Plasmid

   #127088

   Purpose

   Integrating vector containing a tetracycline inducible promoter for use in Streptomyces sp.

   Depositor

   Maggie Smith

   Article

   Rodriguez-Garcia et al Nucleic Acids Res. 2005 May 25;33(9):e87

   Inserts

   tcp830
   TetRiS
   terminator mmrt
   Terminator fdt
   

   Use

   Streptomyce integration vector derived from phic3…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. BA31_pU.CAG.SaCas9-D10A.rBGpA

   Plasmid

   #199251

   Purpose

   Expression construct encoding the S. aureus Cas9.D10A nickase (SaCas9.D10A)

   Depositor

   Manuel Goncalves

   Article

   Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

   Insert

   SaCas9.D10A nickase
   

   Use

   CRISPR

   Tags

   3XHA epitope, Nucleoplasmin NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   CAG promoter

   Available since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pX459V2.0-SpCas9-HF1

   Plasmid

   #108293

   Purpose

   pX459 V2.0 (Plasmid #62988) with the N497A, R661A, Q695A, and Q926A mutations

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pQCasTns(Ptr)-array 8

   Plasmid

   #175580

   Purpose

   Expression of CRISPR-associated transposases

   Depositor

   Sheng Yang

   Article

   Yang et al Nucleic Acids Res. 2021 Sep 3. pii: 6363

   Insert

   PtrTniQ, PtrCas5/8, PtrCas7, PtrCas6, PtrTnsABC, CRISPR(8 CRISPR array)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   NO

   Promoter

   Available since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pREDIT_Cas9-MS2-BB_BbsI

   Plasmid

   #164802

   Purpose

   REDIT backbone for wildtype Cas9, pU6-MS2-gRNA-backbone(BbsI)-CBH-SpCas9-T2A-EBFP

   Depositor

   Le Cong

   Article

   Wang et al Nucleic Acids Res. 2021 Apr 6;49(6):e36.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. BFP Homology Donor

   Plasmid

   #149372

   Purpose

   Promoter-less BFP cDNA with two homology arms to the lenti-CDDR reporter for HDR detection

   Depositor

   Tarek Abbas

   Article

   Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592

   Insert

   CDDR BFP Homology Donor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSLC-238

   Plasmid

   #73192

   Purpose

   Template plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (chpB) under the control of rhamnose induceable promoter (PrhaB) for negative selection.

   Depositor

   Swaine Chen

   Article

   Khetrapal et al Nucleic Acids Res. 2015 Jul 27;43(13):e8

   Insert

   PrhaB-chpB (chpB )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PrhaB

   Available since

   June 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLenti-FNLSHiFiNG-P2A-Puro

    Plasmid

    #136903

    Purpose

    Lentivirus for constitutive expression of FNLSHiFiNG in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    FNLSHiFiNG
    

    Use

    Lentiviral

    Tags

    FLAG-NLS and NLS

    Expression

    Mutation

    R691A,L1111R,D1135V,G1218R,E1219F,A1322R,R1335V,T…

    Promoter

    EF1s

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAID1.2-EF1a-NGFP-mAID

    Plasmid

    #140607

    Purpose

    Expression of OsTIR1 and GFP-mAID protein in mammalian cells under the control of EF1a promoter

    Depositor

    Tatsuo Fukagawa

    Article

    Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

    Insert

    OsTIR1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTmpKmA1O4in

    Plasmid

    #176232

    Purpose

    A template plasmid for amplification of the cI-hok-neo cassette, which contains A1O4 promoter. The promoter is directed toward the cI gene.

    Depositor

    Sergey Sineoky

    Article

    Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89

    Insert

    cI of phage lambda, hok of the R1 plasmid, neo, hybrid PA1O4 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBWB419

    Plasmid

    #140637

    Purpose

    RSF1010-Cas9-pcaHG gRNA. Vector with constitutive Cas9 with gRNA targeting pcaHG locus.

    Depositor

    Keith Tyo

    Article

    Biggs et al Nucleic Acids Res. 2020 Apr 4. pii: 5815

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. IZ501: pMVP/Lenti-DEST

    Plasmid

    #121848

    Purpose

    pMVP destination vector, empty Lentivirus vector backbone

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Lentiviral; Pmvp gateway destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGEM-sgMUC1_dual-MTS-3x-Set I

    Plasmid

    #162763

    Purpose

    Separately expressing 3 unique sgMUC1_dual-MTS (SetI)

    Depositor

    Antony K Chen

    Article

    Mao et al Nucleic Acids Res. 2019 Nov 18;47(20):e1

    Insert

    sgMUC1_dual-MTS_1; sgMUC1_dual-MTS_2; sgMUC1_dual-MTS_3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pB1H2w5-Prd

    Plasmid

    #18039

    Depositor

    Scot Wolfe

    Article

    Noyes et al Nucleic Acids Res. 2008 May;36(8):2547-6

    Insert

    Paired (prd Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Paired is simply used as an insert between Kpn1 a…

    Promoter

    Available since

    June 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pB1H2w2-Prd

    Plasmid

    #18038

    Depositor

    Scot Wolfe

    Article

    Noyes et al Nucleic Acids Res. 2008 May;36(8):2547-6

    Insert

    Paired (prd Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Paired is simply used as an insert between Kpn1 a…

    Promoter

    Available since

    June 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEditC

    Plasmid

    #207532

    Purpose

    Plasmid for cytidine base editing; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→CBE:SpnCas9, PEM7→non-specific sgRNA; SmR/SpR

    Depositor

    Pablo Ivan Nikel

    Article

    Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.

    Insert

    xylS (cured of BsaI-sites), Pm→CBE:SpnCas9, PEM7→non-specific sgRNA
    

    Use

    CRISPR; Pseudomonas vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEditA-RY

    Plasmid

    #207533

    Purpose

    Plasmid for adenine base editing; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→ABE:SpRY, PEM7→non-specific sgRNA; SmR/SpR

    Depositor

    Pablo Ivan Nikel

    Article

    Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.

    Insert

    xylS (cured of BsaI-sites), Pm→ABE:SpRY, PEM7→non-specific sgRNA
    

    Use

    CRISPR; Pseudomonas vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEditC-RY

    Plasmid

    #207534

    Purpose

    Plasmid for cytidine base editing; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→CBE:SpRY, PEM7→non-specific sgRNA; SmR/SpR

    Depositor

    Pablo Ivan Nikel

    Article

    Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.

    Insert

    xylS (cured of BsaI-sites), Pm→CBE:SpRY, PEM7→non-specific sgRNA
    

    Use

    CRISPR; Pseudomonas vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge