We narrowed to 14,699 results for: NTS;

1. pIRES-mCerulean-FRB-KRas/N12-KRas/13C(S17N)-FKBP-mVenus-CAAX

   Plasmid

   #214285

   Purpose

   Express split-KRas fragments that are fused with CIDs and FPs

   Depositor

   Cliff Stains

   Article

   He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

   Inserts

   Split-KRas fragments fused with CIDs and FPs (KRAS Human)
   Split-KRas fragments fused with CIDs and FPs (KRAS Human)
   

   Tags

   mCerulean (N terminal on insert 1) and mVenus (on…

   Expression

   Mammalian

   Mutation

   KRas-S17N

   Promoter

   CMV

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIRES-mCerulean-GID1-KRas/N12-KRas/13C(Q61L)-GAI1-92-mVenus-CAAX

   Plasmid

   #214286

   Purpose

   Express split-KRas fragments that are fused with CIDs and FPs

   Depositor

   Cliff Stains

   Article

   He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

   Inserts

   Split-KRas fragments fused with CIDs and FPs (KRAS Human)
   Split-KRas fragments fused with CIDs and FPs (KRAS Human)
   

   Tags

   mCerulean (N terminal on insert 1) and mVenus (on…

   Expression

   Mammalian

   Mutation

   KRas-Q61L

   Promoter

   CMV

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pIRES-mCerulean-GID1-KRas/N12-KRas/13C(S17N)-GAI1-92-mVenus-CAAX

   Plasmid

   #214287

   Purpose

   Express split-KRas fragments that are fused with CIDs and FPs

   Depositor

   Cliff Stains

   Article

   He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

   Inserts

   Split-KRas fragments fused with CIDs and FPs (KRAS Human)
   Split-KRas fragments fused with CIDs and FPs (KRAS Human)
   

   Tags

   mCerulean (on insert 1) and mVenus (on insert 2)

   Expression

   Mammalian

   Mutation

   KRas-S17N

   Promoter

   CMV

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pT7NT*-Bri2 113-231 R221E

   Plasmid

   #138134

   Purpose

   Expresseds human Bri2 BRICHOS R221E mutant in E. coli

   Depositor

   Jan Johansson

   Article

   Chen et al Commun Biol. 2020 Jan 20;3(1):32. doi: 1

   Insert

   human Bri2 BRICHOS R221E (ITM2B Human)
   

   Tags

   NT* tag derived from spider silk proteins

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. L40C-CRISPR.EFS.mNeon

   Plasmid

   #69146

   Purpose

   Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA (hU6), mNeon coexpression, EFS Promoter driven

   Depositor

   Dirk Heckl

   Article

   Reimer et al Haematologica. 2017 Jun 1. pii: haematol

   Inserts

   SpCas9
   Sp sgRNA scaffold
   EFS (EEF1A1 Human)
   P2A-mNeonGreen
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Available Since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLKO.1-puro U6 sgRNA BfuAI stuffer G-tract

   Plasmid

   #138524

   Purpose

   Expresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt PRC2-binding G-tract repeat RNA sequence.

   Depositor

   Richard Jenner

   Article

   Beltran et al Nat Struct Mol Biol. 2019 Oct;26(10):899

   Insert

   PRC2-binding G-tract repeat RNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLKO.3 Thy1.1

   Plasmid

   #14749

   Depositor

   Christophe Benoist, Diane Mathis

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   April 5, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO.3G

   Plasmid

   #14748

   Depositor

   Christophe Benoist, Diane Mathis

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   April 5, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCFJ352

   Plasmid

   #30539

   Depositor

   Erik Jorgensen

   Insert

   ttTi4348 region
   

   Expression

   Worm

   Available Since

   June 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCFJ210

    Plasmid

    #30538

    Depositor

    Erik Jorgensen

    Insert

    ttTi4348 region
    

    Expression

    Worm

    Available Since

    June 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSARGENT1

    Plasmid

    #216315

    Purpose

    Reporter cassette for SARGENT with piggyBac ITRs and 10x Capture Sequence

    Depositor

    Barak Cohen

    Insert

    tagBFP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. SnFR_S074

    Plasmid

    #182811

    Purpose

    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

    Depositor

    Henry Lester

    Insert

    S074-CholineSnFR
    

    Tags

    6xHis, HA and Myc

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. SnFR_S062

    Plasmid

    #182810

    Purpose

    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

    Depositor

    Henry Lester

    Insert

    S062-CholineSnFR
    

    Tags

    6xHis, HA and Myc

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. CAMP no 3'UTR

    Plasmid

    #129561

    Purpose

    cytomplasmic marker without terminator (less integration)

    Depositor

    Inaki Ruiz-Trillo

    Insert

    mCherry
    

    Expression

    Bacterial

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRSET.HaloTag.v7.AviTag

    Plasmid

    #195486

    Purpose

    Bacterial expression plasmid with Halotag v7 insert plus a C-terminus appended AviTag for enzymatic biotinylation.

    Depositor

    Tim Brown, HHMI-JRC Tool Translation Team

    Insert

    HaloTag.v7.AviTag
    

    Tags

    6xHIS and AviTag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPtGG-1

    Plasmid

    #89990

    Purpose

    pBR322-based entry vector for Gateway cloning

    Depositor

    Philip Weyman

    Article

    Bielinski et al PeerJ Preprints. 2017 5:e2907v1

    Type

    Empty backbone

    Use

    Golden gate entry vector

    Available Since

    June 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. SnFR_V.7.1.2

    Plasmid

    #182809

    Purpose

    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

    Depositor

    Henry Lester

    Insert

    V.7.1.2-CholineSnFR
    

    Tags

    6xHis, HA and Myc

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pNCA

    Plasmid

    #17363

    Depositor

    Stephen Goff

    Article

    Colicelli et al J Mol Biol. 1988 Jan 5. 199(1):47-59.

    Insert

    MMLV proviral DNA
    

    Available Since

    March 1, 2010

    Availability

    Academic Institutions and Nonprofits only

19. KP.3

    Plasmid

    #233340

    Purpose

    expressing SARS-CoV-2 KP.3 spike protein for pseudovirus production

    Depositor

    David Nemazee

    Insert

    Spike (KP.3) (S SARS-CoV-2)
    

    Expression

    Mammalian

    Mutation

    F456L, Q493E, V1104L on the basis of JN.1

    Promoter

    CMV

    Available Since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-luc-miR21(P)

    Plasmid

    #20382

    Depositor

    Bryan Cullen

    Article

    Zeng et al RNA. 2003 Jan . 9(1):112-23.

    Insert

    mir21(P) targets
    

    Expression

    Mammalian

    Available Since

    May 5, 2009

    Availability

    Academic Institutions and Nonprofits only