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pLV-CS-119

Plasmid

#197135

Purpose

CloneSelect A-to-G reporter encoding three target barcodes (3-input AND gate circuit)

Depositor

Nozomu Yachie

Article

Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

Insert

EGFP

Use

Lentiviral

Expression

Mammalian

Mutation

EGFP(M1V)

Promoter

CMV

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmThor_50-83-YMVLtoAADD_AB

Plasmid

#148376

Purpose

Insect Expression of DmThor_50-83-YMVLtoAADD

Depositor

Elisa Izaurralde

Article

Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

Insert

DmThor_50-83-YMVLtoAADD (Thor Fly)

Expression

Insect

Mutation

two silent mutations compared to the sequence giv…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmThor-V75DL79D_AB

Plasmid

#148377

Purpose

Insect Expression of DmThor-V75DL79D

Depositor

Elisa Izaurralde

Article

Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

Insert

DmThor-V75DL79D (Thor Fly)

Expression

Insect

Mutation

two silent mutations compared to the sequence giv…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmThor-S65DT70D_AB

Plasmid

#148375

Purpose

Insect Expression of DmThor-S65DT70D

Depositor

Elisa Izaurralde

Article

Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

Insert

DmThor-S65DT70D (Thor Fly)

Expression

Insect

Mutation

two silent mutations compared to the sequence giv…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pMeCP2Y pc644

Plasmid

#167565

Purpose

The complete rat MeCp2 ORF is fused in frame of the NH2-terminus of the enhanced YFP (pEYFP-N1 vector; CLONTECH Laboratories, Inc.)

Depositor

Cristina Cardoso

Article

Brero et al J Cell Biol. 2005 Jun 6;169(5):733-43. d

Insert

Methyl-CpG binding protein 2 (Mecp2 Rat)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pv9_MBD1_MBD-R22A-13XL-BASU

Plasmid

#179417

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MBD1.MBD-R22A-13XL-BASU

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

MBD1.MBD-R22A-13XL-BASU

Expression

Mammalian

Promoter

CAGGS

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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1N/2CxNLS-cMyc-NLP-cMyc enAspCas12a

Plasmid

#182126

Purpose

pET21a protein expression vector for 1N/2CxNLS-cMyc-NLP-cMyc enAspCas12a in bacteria

Depositor

Scot Wolfe

Article

Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

Insert

1N/2CxNLS-cMyc-NLP-cMyc AspCas12aen

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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1N/2CxNLS-cMyc-NLP-cMyc AspCas12a

Plasmid

#182121

Purpose

pET21a protein expression vector for 1N/2CxNLS-cMyc-NLP-cMyc AspCas12a in bacteria

Depositor

Scot Wolfe

Article

Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

Insert

1N/2CxNLS-cMyc-NLP-cMyc AspCas12a

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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1N/2CxNLS-cMyc-NLP-cMyc LbaCas12a

Plasmid

#182124

Purpose

pET21a protein expression vector for 1N/2CxNLS-cMyc-NLP-cMyc LbaCas12a in bacteria

Depositor

Scot Wolfe

Article

Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

Insert

1N/2CxNLS-cMyc-NLP-cMyc LbaCas12a

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2326

Plasmid

#169628

Purpose

Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' insulator sequence derived from the pGL3 plasmid (PhCMV-SEAP-p2A-iRFP670-pA::pGL3-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven expression of SEAP and iRFP and pGL3-modulated PPGK-driven expression of secreted Nluc and mTagBFP2

Expression

Mammalian

Promoter

PhCMV / PPGK

Available Since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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