Addgene

We narrowed to 154 results for: BTK

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pDONR223-BTK

Plasmid

#23918

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

BTK (BTK Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pBTK503

Plasmid

#110616

Purpose

BTK assembled broad-host-range plasmid encoding GFP driven by CP25 promoter

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

GFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pBTK107

Plasmid

#110575

Purpose

BTK Type 2 promoter for golden gate assembly

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

CP25 Promoter with RBS

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK301

Plasmid

#110593

Purpose

BTK Type 6-7 terminator (replaces Type 6 and Type 7 parts)

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

Bba_B0015 Terminator

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK1050

Plasmid

#209540

Purpose

BTK Type 1 homology arm for Golden Gate assembly

Depositor

Jeffrey Barrick

Article

Lariviere et al bioRxiv 2023.09.19.558440

Insert

Upstream homology arm for recombination into the S. alvi wkB2 genome w/in the SALWKB2_RS11215/SALWKB2_RS11220 intergenic region

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK1051

Plasmid

#209541

Purpose

BTK Type 5 homology arm for Golden Gate assembly

Depositor

Jeffrey Barrick

Article

Lariviere et al bioRxiv 2023.09.19.558440

Insert

Recombination into the S. alvi wkB2 genome within the SALWKB2_RS11215/SALWKB2_RS11220 intergenic region

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 2, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pHH0103_BTK-1/1

Plasmid

#91350

Purpose

Protein expression and purification of human SH3 domain construct BTK-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

BTK-1/1 (BTK Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pBTK622

Plasmid

#180592

Purpose

tdk/kanR dual selection cassette for engineering the genome of Acinetobacter baylyi ADP1

Depositor

Jeffrey Barrick

Article

Suarez et al Nucleic Acids Res. 2020 May 7;48(8):4585

Inserts

tdk
kanR

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 19, 2022

Availability

Academic Institutions and Nonprofits only

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pBTK519

Plasmid

#110603

Purpose

BTK assembled plasmid- broad-host-range GFP expression from PA1 promoter (kanamycin resistance)

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

GFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pBTK552

Plasmid

#110618

Purpose

BTK assembled broad-host-range plasmid encoding inducible GFP driven CP25 promoter with lac operator. Also encodes LacI.

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

GFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223_BTK_WT

Plasmid

#82197

Purpose

Gateway Donor vector containing BTK , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

BTK (BTK Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pBTK402

Plasmid

#110598

Purpose

BTK Type 8 Bacterial origin (broad-host-range) for golden gate assembly

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

mRFP dropout (RSF1010 origin)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pBTK569

Plasmid

#110614

Purpose

BTK assembled broad-host-range plasmid encoding E2-Crimson protein driven by CP25 promoter

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

E2-Crimson

Use

Tags

Expression

Bacterial

Mutation

V32M (see comments below)

Promoter

Available since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pBTK800

Plasmid

#179209

Purpose

GFP dropout for assembling a double-stranded RNA expression plasmid

Depositor

Jeffrey Barrick

Article

Lariviere et al Nat Protoc. 2023 Mar;18(3):902-928. doi:

Insert

GFP dropout

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 19, 2022

Availability

Academic Institutions and Nonprofits only

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pBTK229

Plasmid

#110591

Purpose

BTK Type 3 coding sequence for golden gate assembly and homologous recombination

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

Kanamycin resistance

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK501

Plasmid

#110602

Purpose

BTK assembled plasmid- broad-host-range GFP expression from PA1 promoter (ampicillin resistance)

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

GFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBTK527

Plasmid

#110605

Purpose

BTK Assembled plasmid- ready for Stage 2 assembly (ConLS' and ConRE' connectors with kanamycin resistance)

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK570

Plasmid

#110615

Purpose

BTK assembled broad-host-range plasmid encoding E2-Crimson protein driven by PA3 promoter

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

E2-Crimson

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pBTK510

Plasmid

#110611

Purpose

BTK assembled broad-host-range plasmid encoding GFP driven by PA3 promoter

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

GFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pBTK564

Plasmid

#110613

Purpose

BTK assembled broad-host-range plasmid encoding NanoLuc luciferase protein driven by PA1 promoter

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

Nanoluc

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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