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Addgene
Showing: 61 - 80 of 122 results
  1. pSCrhaB2plus

    Plasmid
    #164226
    Purpose
    Broad-range bacterial expression vector with modified rhamnose-inducible promoter. Harbours enhanced rhaS binding site and encodes T7 gene10 stem loop in mRNA for enhanced expression.
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Rhamnose-inducible
    Availability
    Academic Institutions and Nonprofits only
  2. pGPΩTp

    Plasmid
    #130660
    Purpose
    Insertional mutagenesis of GP transposon into chromosome of B. cenocepacia. Trimethoprim selection for plasmid.
    Depositor
    Insert
    Ω trimethoprim cassette
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Addition of Ω sites next to trimethoprim cassette…
    Promoter
    dhfr promoter
    Availability
    Academic Institutions and Nonprofits only
  3. pAH-CTX1-rhadCas9-native

    Plasmid
    #129392
    Purpose
    Derived from pAH-CTX1-rha. The native Streptococcus pyogenes dCas9 gene cloned downstream of PrhaBAD. Non-codon-optimized dCas9 version of pAH-CTX1-rhadCas9.
    Depositor
    Insert
    S. pyogenes dCas9
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Aspartate 10 to Alanine, Histidine 840 to Alanine
    Promoter
    PrhaBAD
    Availability
    Academic Institutions and Nonprofits only
  4. pAH18

    Plasmid
    #154397
    Purpose
    Derived from pGPI-SceI; Trimethoprim resistant; carries I-SceI gene; contains 475 bp from both upstream and downstream flanking regions between the FRT sites on pAHCTX1-rhadCas9
    Depositor
    Insert
    Fused upstream and downstream homologous regions around FRT sites when pAH-CTX1-rhadCas9 integrated into K56-2 genome
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    NA
    Availability
    Academic Institutions and Nonprofits only
  5. pBBR1-Rha-ETh_tji49-kan

    Plasmid
    #166669
    Purpose
    Genome engineering of Burkholderia
    Depositor
    Insert
    ETh_tji4
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  6. pBBR1-Rha-ETh1h2e_yi23-kan

    Plasmid
    #166670
    Purpose
    Genome engineering of Burkholderia
    Depositor
    Insert
    ETh1h2e_yi23
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
Showing: 61 - 80 of 122 results