We narrowed to 15 results for: arlene sharpe

1. pLX_305_Ovalbumin

   Plasmid

   #184924

   Purpose

   Lentiviral vector for expressing Ovalbumin. Also expresses the puromycin resistance gene as a selectable marker.

   Depositor

   Arlene Sharpe

   Article

   Milling et al J Exp Med. 2024 Apr 1;221(4):e20230699.

   Insert

   Ovalbumin (OVAL Chicken)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Our vector has an A188T mutation that does not im…

   Promoter

   PGK

   Available since

   Feb. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pXPR_071

   Plasmid

   #164558

   Purpose

   Higher titer version of pXPR_053. Lentiviral vector that enables constitutive sgRNA expression. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.

   Depositor

   Arlene Sharpe

   Article

   LaFleur et al Nat Immunol. 2023 Nov 27. doi: 10.1038/s

   Insert

   sgRNA cassette
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6

   Available since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRDA_526

   Plasmid

   #184859

   Purpose

   Lentiviral vector that enables constitutive sgRNA expression. Also encodes Thy1.1 as a marker of transduction.

   Depositor

   Arlene Sharpe

   Article

   Milling et al J Exp Med. 2024 Apr 1;221(4):e20230699.

   Insert

   sgRNA cassette
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6

   Available since

   Feb. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pXPR_219

   Plasmid

   #164559

   Purpose

   Lentiviral vector that encodes two sgRNA expression cassettes. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.

   Depositor

   Arlene Sharpe

   Article

   LaFleur et al Nat Immunol. 2023 Nov 27. doi: 10.1038/s

   Inserts

   First sgRNA cassette
   Second sgRNA cassette
   

   Use

   CRISPR and Lentiviral; Dual sgrna

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6 and Mouse U6

   Available since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pXPR_070

   Plasmid

   #164265

   Purpose

   Lentiviral vector that enables Cre-mediated sgRNA expression. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.

   Depositor

   Arlene Sharpe

   Article

   LaFleur et al Nat Immunol. 2023 Nov 27. doi: 10.1038/s

   Insert

   sgRNA cassette
   

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6

   Available since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pXPR_053

   Plasmid

   #113591

   Purpose

   Constitutive expression of a single sgRNA and the fluorophore violet-excited GFP in immune cells

   Depositor

   Arlene Sharpe

   Article

   LaFleur et al Nat Commun. 2019 Apr 10;10(1):1668. doi:

   Insert

   sgRNA cloning site
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRRL-SFFV_V5-CHIC2_IFRPDdel_Tomato

   Plasmid

   #238126

   Purpose

   Expresses V5-tagged CHIC2 (with IFRPD deletion) with Tomato selectable marker.

   Depositor

   Arlene Sharpe

   Insert

   V5-tagged CHIC2 with IFRPD deletion
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Deleted amino acids 160-165 (IFRPD).

   Promoter

   SFFV

   Available since

   July 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRRL-SFFV_V5-CHIC2-K117R_Tomato

   Plasmid

   #238127

   Purpose

   Expresses V5-tagged CHIC2 (with K117R mutation) with Tomato selectable marker.

   Depositor

   Arlene Sharpe

   Insert

   V5-tagged CHIC2 with K117R mutation
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Mutated Lysine 117 to Arginine.

   Promoter

   SFFV

   Available since

   July 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRRL-SFFV_V5-CHIC2-C6S_Tomato

   Plasmid

   #238128

   Purpose

   Expresses V5-tagged CHIC2 (with C6S mutation) with Tomato selectable marker.

   Depositor

   Arlene Sharpe

   Insert

   V5-tagged CHIC2 with C6S mutation
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Changed cysteines from amino acids 102-109 to ser…

   Promoter

   SFFV

   Available since

   July 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRRL-SFFV_STUB1-UBOXdel-V5_Tomato

    Plasmid

    #238129

    Purpose

    Expresses V5-tagged STUB1 (with UBOX deletion) with Tomato selectable marker.

    Depositor

    Arlene Sharpe

    Insert

    V5-tagged-STUB1
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Deleted UBOX domain (amino acids 224-294)

    Promoter

    SFFV

    Available since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLX_305_OVA_Blasticidin

    Plasmid

    #238124

    Purpose

    Enables expression of Ovalbumin with Blasticidin selectable marker.

    Depositor

    Arlene Sharpe

    Insert

    Ovalbumin (OVAL Chicken)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human PGK

    Available since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRRL-SFFV_V5-CHIC2_Tomato

    Plasmid

    #238125

    Purpose

    Expresses V5-tagged CHIC2 with Tomato selectable marker.

    Depositor

    Arlene Sharpe

    Insert

    V5-tagged CHIC2
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRDA_852

    Plasmid

    #185527

    Purpose

    Lentiviral vector that enables Cre-mediated expression of two sgRNAs. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.

    Depositor

    Arlene Sharpe

    Inserts

    First sgRNA cassette
    Second sgRNA cassette
    

    Use

    CRISPR, Cre/Lox, and Lentiviral ; Flp/frt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6 and Mouse U6

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pXPR_068

    Plasmid

    #164555

    Purpose

    Lentiviral vector that enables Flp-mediated sgRNA expression. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.

    Depositor

    Arlene Sharpe

    Article

    LaFleur et al Nat Immunol. 2023 Nov 27. doi: 10.1038/s

    Insert

    sgRNA cassette
    

    Use

    CRISPR and Lentiviral; Flp/frt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6

    Available since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pXPR_220

    Plasmid

    #164560

    Purpose

    Lentiviral vector that encodes two sgRNA expression cassettes (one constitutive, one inducible via Flp). Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.

    Depositor

    Arlene Sharpe

    Article

    LaFleur et al Nat Immunol. 2023 Nov 27. doi: 10.1038/s

    Inserts

    First sgRNA cassette
    Second sgRNA cassette
    

    Use

    CRISPR and Lentiviral; Flp/frt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6 and Mouse U6

    Available since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge