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We narrowed to 16 results for: half life

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Showing: 1 - 16 of 16 results

  1. Lentiviral-TOP-dGFP-reporter

    Plasmid
    #14715
    Purpose
    Beta-catenin reporter - 3rd generation self-inactivating lentiviral plasmid LEF-1/TCF-responsive promoter driven d2-eGFP (destabilized, half-life of 2h).
    Depositor
    Tannishtha Reya
    Publication
    Reya et al Nature. 2003 May 22. 423(6938):409-14.
    Insert
    LEF-1/TCF-responsive promoter (Ctnnb1 )
    Use
    Lentiviral
    Tags
    d2 eGFP
    Expression
    Mammalian
    Available since
    Aug. 28, 2009
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    14715-plasmid-map-sequence-id-339924

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  2. CMV-LUC2CP/intron/ARE

    Plasmid
    #62858
    Purpose
    splicing reporter (with intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.
    Depositor
    Gideon Dreyfuss
    Publication
    Younis et al Mol Cell Biol. 2010 Apr;30(7):1718-28. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Luciferase
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    insertion of chimeric intron at nueotide poclsiti…
    Promoter
    CMV
    Available since
    April 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    62858-plasmid-map-sequence-id-243538

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  3. CMV-LUC2CP/ARE

    Plasmid
    #62857
    Purpose
    Splicing reporter control (no intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.
    Depositor
    Gideon Dreyfuss
    Publication
    Younis et al Mol Cell Biol. 2010 Apr;30(7):1718-28. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Luciferase
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    destabilizing sequences (DS) added to the C termi…
    Promoter
    CMV
    Available since
    April 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    62857-plasmid-map-sequence-id-212900

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  9. pOpen-GBDpol (exo)

    Plasmid
    #165572
    Purpose
    Robust and extremely thermostable polymerase with a half-life of 23 hours at 95 degrees C; offers 5x higher fidelity than Taq and robust performance. Ideal for GC-rich or looped sequences. Lacks exonuclease activity. Comparable to Deep Vent (exo-) DNA Polymerase at NEB
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Pyrococcus Sp. Heat-Stable (exo-) DNA Polymerase
    Use
    Synthetic Biology
    Available since
    Aug. 24, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165572-plasmid-map-sequence-id-340932

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  11. FcD265A-FLAG

    Plasmid
    #65420
    Purpose
    Mammalian expression plasmid for murine Fc-FLAG, used for monomeric Fc-IL2 production
    Depositor
    Dane Wittrup
    Publication
    Zhu et al Cancer Cell. 2015 Apr 13;27(4):489-501.
    Insert
    FcD265A-FLAG
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    D265A
    Promoter
    Modified CMV Promoter
    Available since
    June 9, 2015
    Availability
    Academic Institutions and Nonprofits only

  14. pmGFP

    Plasmid
    #188451
    Purpose
    Expression of a modified GFP (mGFP) with short half-life
    Depositor
    Ajit Chande
    Publication
    Mishra et al Mol Ther Nucleic Acids. 2022 Mar 8;28:20
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cyclin destruction box
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    188451-plasmid-map-sequence-id-372346

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  15. KBB106

    Plasmid
    #185065
    Purpose
    Bacterial expression of C-terminus of NUP1 nucleoporin as GST-Nup1-C fusion including C-terminal half of Nup1 C-terminal FG domain
    Depositor
    Ken Belanger
    Publication
    Belanger et al CBE Life Sci Educ. 2009 Fall;8(3):214-25
    Insert
    NUP1
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Nup1 C-terminal region, truncated so C-terminal h…
    Available since
    July 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    185065-plasmid-map-sequence-id-367180

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  16. O-FHL1-1

    Plasmid
    #166543
    Purpose
    scFv of a human scaffold targeting Four and a half LIM domains protein 1. Antigen coverage aa 1-280 aa, full-length
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    166543-plasmid-map-sequence-id-328279

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Showing: 1 - 16 of 16 results