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Addgene
Showing: 41 - 60 of 87 results

  1. 4R3H

    Plasmid
    #64652
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    YTHDC1:JMC092-F09:C231531 (YTHDC1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only

  4. pTXBI-mclover-YTH-IDR2

    Plasmid
    #177122
    Purpose
    Bacterial expression of YTH-IDR2 fragment of YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purification
    Depositor
    Wenbo Li
    Article
    Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e
    Insert
    YTHDC1 (YTH and IDR2 domains) (YTHDC1 Human)
    Use
    Tags
    Mxe GyrA intein-Chitin binding domain for protein…
    Expression
    Bacterial
    Mutation
    YTH and IDR2 domains only (IDR1 deletion)
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    177122-plasmid-map-sequence-id-348676

    Enlarge

  5. pTXBI-mClover-YTH domain

    Plasmid
    #177123
    Purpose
    Bacterial expression of YTH domain of YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purification
    Depositor
    Wenbo Li
    Article
    Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e
    Insert
    YTHDC1 (YTH domain) (YTHDC1 Human)
    Use
    Tags
    Mxe GyrA intein-Chitin binding domain for protein…
    Expression
    Bacterial
    Mutation
    YTH domain only (IDR1 and IDR2 deletion)
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    177123-plasmid-map-sequence-id-348690

    Enlarge

  7. pD649-HAsp-CLEC4A-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156564
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    CLEC4A (CLEC4A Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Availability
    Academic Institutions and Nonprofits only
    156564-plasmid-map-sequence-id-368735

    Enlarge

  8. rnALDH

    Plasmid
    #67920
    Purpose
    Expresses aldehyde dehydrogenase from Rattus norvegicus, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Insert
    Rattus norvegicus aldehyde dehydrogenase (Aldh3a1 Rat)
    Use
    Tags
    6x-His
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only

  9. PARP12A

    Plasmid
    #42383
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PARP12A (PARP12 Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  16. EcMscL

    Plasmid
    #92418
    Purpose
    E. coli Mechanosensitive Channel of Large Conductance
    Depositor
    Douglas Rees
    Insert
    MscL (mscL E. coli)
    Use
    Tags
    6x Histidine Tag and Thrombin Cleavage Site betwe…
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Availability
    Academic Institutions and Nonprofits only
    92418-plasmid-map-sequence-id-183401

    Enlarge

  17. p416-GPD-DIPP2-HA

    Plasmid
    #183947
    Purpose
    Expresses human HA-tagged DIPP2 from yeast GPD promoter
    Depositor
    Michael Downey
    Insert
    DIPP2 (NUDT4 Human)
    Use
    Tags
    HA
    Expression
    Yeast
    Mutation
    WT
    Promoter
    GPD
    Availability
    Academic Institutions and Nonprofits only
    183947-plasmid-map-sequence-id-363373

    Enlarge

  18. pLKO.1 LARP7-FLAG 7SK

    Plasmid
    #200733
    Purpose
    Lentiviral expression of Flag-Tagged human LARP7 and the snRNA 7SK
    Depositor
    Claudio Alarcón
    Insert
    LARP7 and 7SK (LARP7 Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Availability
    Academic Institutions and Nonprofits only
    200733-plasmid-map-sequence-id-401803

    Enlarge

  19. rnALDH

    Plasmid
    #67920
    Purpose
    Expresses aldehyde dehydrogenase from Rattus norvegicus, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Insert
    Rattus norvegicus aldehyde dehydrogenase (Aldh3a1 Rat)
    Use
    Tags
    6x-His
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
Showing: 41 - 60 of 87 results