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Flag-TurboID

Plasmid

#124646

Purpose

Expresses Flag-TurboID fusion for proximity labeling

Depositor

FENG-QIAN Li, Ken-Ichi Takemaru

Type

Empty backbone

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2019

Availability

Academic Institutions and Nonprofits only

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TurboID-His6_pET21a

Plasmid

#107177

Purpose

expresses His6-tagged TurboID in bacteria

Depositor

Alice Ting

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

TurboID (BirA mutant)

Expression

Bacterial

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Available Since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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TurboID-V5_pRS415

Plasmid

#107167

Purpose

expresses V5-tagged TurboID in the yeast cytosol

Depositor

Alice Ting

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

TurboID (BirA mutant)

Expression

Yeast

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Available Since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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p3E-TurboID

Plasmid

#166570

Purpose

Multisite Gateway 3' entry clone for fusions onto the N-terminus of TurboID. Parton lab clone JSS

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

TurboID

Use

Multisite gateway entry clone

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pet21_TurboID_18A

Plasmid

#170922

Purpose

Express TurboID_18A in Bl21

Depositor

Huan Bao

Article

Bao et al Commun Biol. 2021 Apr 30;4(1):514. doi:

Insert

TurboID-18a

Expression

Bacterial

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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V5-TurboID-NES_pCDNA3

Plasmid

#107169

Purpose

expresses V5-tagged TurboID in the mammalian cytosol

Depositor

Alice Ting

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

TurboID (BirA mutant)

Expression

Mammalian

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Available Since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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Sec61b-V5-TurboID

Plasmid

#166971

Purpose

Expresses ER targeted TurboID for secretome biotinylation

Depositor

Hyun-Woo Rhee

Article

Kim et al Nat Commun. 2021 Sep 1;12(1):5204. doi:

Insert

Sec61b-V5-TurboID

Tags

V5

Expression

Mammalian

Promoter

CMV promoter

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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C1(1-29)-TurboID-V5_pCDNA3

Plasmid

#107173

Purpose

expresses V5-tagged TurboID on the mammalian ER membrane

Depositor

Alice Ting

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

TurboID (BirA mutant)

Expression

Mammalian

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Available Since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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C1(1-29)-TurboID-V5_pLX304

Plasmid

#107175

Purpose

expresses V5-tagged TurboID on the mammalian ER membrane, lentiviral vector

Depositor

Alice Ting

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

TurboID (BirA mutant)

Use

Lentiviral

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Available Since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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3xHA-TurboID_pAS31

Plasmid

#118220

Purpose

expresses 3xHA-tagged TurboID in C. elegans intestine

Depositor

Jessica Feldman

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

TurboID (BirA mutant)

Tags

3x HA tag

Expression

Worm

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Promoter

ges-1p

Available Since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pME-TurboID-NS

Plasmid

#166566

Purpose

Multisite Gateway middle entry clone encoding TurboID. Parton lab clone JRP

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

TurboID

Use

Multisite gateway entry clone

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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3xHA-TurboID-NLS_pCDNA3

Plasmid

#107171

Purpose

expresses 3xHA-tagged TurboID in the mammalian nucleus

Depositor

Alice Ting

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

TurboID (BirA mutant)

Expression

Mammalian

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Available Since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEx-ER-TurboID

Plasmid

#160857

Purpose

Cre recombinase-dependent expression of ER TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection

Depositor

Jonathan Long

Article

Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

Has Service

AAV8

Insert

ER TurboID

Use

AAV

Tags

HA and V5

Promoter

CAG

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-TBG-ER-TurboID

Plasmid

#149415

Purpose

Hepatocyte-specific expression of ER-localized TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection

Depositor

Jonathan Long

Article

Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

Has Service

AAV8

Insert

ER-TurboID

Use

AAV

Tags

HA and V5

Promoter

TBG

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-tMCK-ER-TurboID

Plasmid

#149412

Purpose

Muscle-specific expression of ER-localized TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection

Depositor

Jonathan Long

Article

Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

Insert

ER-TurboID

Use

AAV

Tags

HA and V5

Promoter

tMCK

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mem-TurboID

Plasmid

#149409

Purpose

Mammalian expression of secretory pathway membrane-localized TurboID, N-term HA tag, C-term V5 tag, in mammalian expression plasmid. Amp/Neo selection

Depositor

Jonathan Long

Article

Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

Insert

mem-TurboID

Tags

HA and V5

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pUAS-V5-miniTurboID-NES

Plasmid

#116905

Purpose

Expresses miniTurboID under Gal4/UAS control. miniTurboID is tagged at the N-terminus with V5 and a nuclear export signal (NES) at the C-terminus.

Depositor

Norbert Perrimon

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

miniTurboID

Use

Drosophila expression

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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3xHA-TurboID pRetrox

Plasmid

#155203

Purpose

For doxycycline-inducible expression of 3xHA-TurboID on N-terminus of bait protein

Depositor

Kyle Roux

Article

May et al Cells. 2020 Apr 25;9(5). pii: cells90510

Insert

3xHA-TurboID

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Promoter

tight TRE promotor

Available Since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pUAS-V5-TurboID-NES

Plasmid

#116904

Purpose

Expresses TurboID under Gal4/UAS control. TurboID is tagged at the N-terminus with V5 and a nuclear export signal (NES) at the C-terminus.

Depositor

Norbert Perrimon

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

TurboID

Use

Drosophila expression

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA5_FRT_TO_TurboID-mCherry-Cry2

Plasmid

#166504

Purpose

Expression of your protein of interest fused with TurboID and mcherry-CRY2 for optogenetic activation, Flp-In T-Rex system. Search for client proteins within light-induced biomolecular condensates.

Depositor

Angelos Constantinou

Article

Frattini et al Mol Cell. 2021 Jan 16. pii: S1097-2765(2

Insert

None

Tags

TurboID and mCherry-CRY2

Expression

Mammalian

Available Since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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