158 results
-
Plasmid Kit#1000000107PurposeA series of pCS2+ based Gateway vectors with N- or C-terminal tags (eg Myc, HA, Flag, GST and eGFP), which allow the generation of tagged proteins in a variety of vertebrate organisms.DepositorAvailable sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only
-
MultiSite Gateway plasmid toolKit
Plasmid Kit#1000000050PurposeCloning kit for constructing fully-customizable three-part fusion proteins, based on MultiSite Gateway from Life Technologies. 12 promoter-less Gateway entry clones, N- and C-term flanking modulesDepositorAvailable sinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
Target Accelerator Genetic Pathway Reference Set
Plasmid Kit#1000000105PurposeCollection of genes and mutations for reference pathway signatures and phenotypes to compare with those derived from cancer alleles; represent over 25 cellular signaling pathways and processesDepositorAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
Hamdoun lab FP fusion plasmids
Plasmid Kit#1000000033PurposeScreen fluorescent protein fusion expression in organisms or cells in which an exogenous mRNA can be injected and expressed, e.g. zebrafish, sea urchin, frog (Xenopus), or worm (C. elegans)DepositorAvailable sinceJuly 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
Start-Stop Assembly Toolkit
Plasmid Kit#1000000152PurposeStart-Stop Assembly is a multi-part, modular DNA assembly method which is functionally scarless, suitable for combinatorial assembly, and ideal for metabolic engineering in diverse organisms.DepositorAvailable sinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
BIOFAB
Plasmid Kit#1000000037PurposeSets of specific constitutive bacterial promoters that vary in strength, followed by specific Bi-Cistronic Design elements varying in effectiveness to drive E. coli expression of a gene of interestDepositorAvailable sinceDec. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
Zinc Finger Consortium OPEN Accessory Reagent Set
Plasmid Kit#1000000013PurposeContains plasmids and strains used to engineer Zinc Finger Nucleases (ZFNs) via the Oligomerized Pool ENgineering (OPEN) method.DepositorAvailable sinceJuly 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
FNL Combinatorial Cloning Platform
Plasmid Kit#1000000211PurposeA collection of Multisite Gateway Entry clone plasmids which can be used in a combinatorial fashion to generate large numbers of tagged expression clones for protein expression in a variety of systemsDepositorAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
cpFRET
Plasmid Kit#1000000021Purposecircular permutation-based fluorescence resonance energy transfer (cpFRET) biosensor toolkit: generate and optimize genetically encoded, unimolecular FRET sensors for ratiometric measurementsDepositorAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
Ralstonia-GG-Kit
Plasmid Kit#1000000142PurposeBackbone vectors and Level I modules that can be used for assembly of Ralstonia solanacearum species complex gene knockout and complementation constructs based on Golden Gate cloning techniquesDepositorAvailable sinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
AspFlex Kit
Plasmid Kit#1000000217PurposeThis collection is a set of Modular Cloning (MoClo) plasmids for Acinetobacter spp. and Acinetobacter baumannii for protein expression analyses and CRISPRi experiments.DepositorAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
STAR TALE Assembly Kit
Plasmid Kit#1000000117PurposeThese plasmids use a TALE assembly reaction (STAR) method to construct multiple TALEs in a fast and simple manner.DepositorAvailable sinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
Plasmid-ID Kit
Plasmid Kit#1000000168PurposeThe Plasmid-ID Kit allows for the high-throughput assessment of competitive and effective rhizobial strains.DepositorAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
CRISPRecise Kit
Plasmid Kit#1000000235PurposeCRISPRecise is a set of high-fidelity SpCas9 variants that can be used to efficiently edit any SpCas9 target with practically no detectable off-target effects.DepositorAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
OVC Core Collection
Plasmid Kit#1000000164PurposeThree plasmids with the empty cloning cassette in configurations ABC, BAC or ACB, and 21 plasmids containing one of nine light-sensing domains (LSDs) or LSD binding partners.DepositorAvailable sinceOct. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
Musunuru-Cowan TALEN Kit
Plasmid Kit#1000000034PurposeUsed to efficiently assemble TALEN constructs with custom repeat arrays, each containing 15 repeats. Contains pre-assembled trimer and tetramer RVD domains designed for rapid TALEN assembly.DepositorAvailable sinceJuly 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
Barcoded self-reporting transposon calling card collection
Plasmid Kit#1000000213Purpose'Calling Cards' technology using self-reporting transposons (SRTs) enables the identification of DNA-protein interactions through RNA sequencing.DepositorAvailable sinceFeb. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
dRMCE Plasmids
Plasmid Kit#1000000015PurposeTakes advantage of wild-type loxP and FRT sites present in these conditional alleles in mouse embryonic stem cells to introduce tags, reporters, and mutant coding regions into an endogenous lociDepositorAvailable sinceJuly 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
FREQ-Seq Kit
Plasmid Kit#1000000032PurposeFor construction of barcoded, locus-specific libraries compatible with Illumina next generation sequencing. Allows for direct quantitative allele detection. 48 plasmid adaptors (barcoded M13F primer)DepositorAvailable sinceMay 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
Ekker Lab TALEN kit
Plasmid Kit#1000000038PurposeContains all 256 possible combinations of pFUS_B4 clones for Golden Gate assembly of 15-RVD TALENs for high-throughput mutagenesisDepositorAvailable sinceMarch 14, 2014AvailabilityAcademic Institutions and Nonprofits only