155 results

1. Golden GATEway Cloning Kit

   Plasmid Kit

   #1000000054

   Purpose

   Combinatorial assembly method that combines Golden Gate (Type IIs endonucleases) cloning with Multisite Gateway (recombination) cloning to construct complex plasmids in an efficient and modular way

   Depositor

   Joachim Wittbrodt

   Available since

   Sept. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

2. Multiple Lentiviral Expression (MuLE) system

   Plasmid Kit

   #1000000060

   Purpose

   Multiple Lentiviral Expression (MuLE): modular system used to construct complex lentiviruses for modification of mammalian cells with a single viral infection.

   Depositor

   Ian Frew

   Available since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

3. EMMA toolkit

   Plasmid Kit

   #1000000119

   Purpose

   Used for the construction of a variety of mammalian expression vectors in a one-pot, one-step Golden Gate reaction; compatible with automated production

   Depositor

   Yizhi Cai

   Available since

   March 1, 2018

   Availability

   Academic Institutions and Nonprofits only

4. Endy/Shearwin pOSIP plasmid set

   Plasmid Kit

   #1000000035

   Purpose

   11 Plasmids for use in clonetegration: method for decreasing time and effort needed for integration of DNA into prokaryotic chromosomes. Facilitates cloning, expression of difficult genetic elements

   Depositor

   Drew Endy

   Available since

   July 26, 2013

   Availability

   Academic Institutions and Nonprofits only

5. S. cerevisiae Advanced Gateway Destination Vectors

   Plasmid Kit

   #1000000011

   Purpose

   Yeast (S. cerevisiae) Gateway Destination Vectors for tagging and gene expression. Has variety of promoters, origin of replications, auxotrophic markers, and N- or C-terminal fusion proteins

   Depositor

   Susan Lindquist

   Available since

   July 16, 2011

   Availability

   Academic Institutions and Nonprofits only

6. Yeast Prototrophy Kit

   Plasmid Kit

   #1000000085

   Purpose

   Plasmids derived from the pHLUM mini-chromosome, designed to restore prototrophy (eg HIS3, LEU2, URA3, MET17 or LYS2 genes) in common yeast (S. cerevisiae) laboratory strains.

   Depositor

   Markus Ralser

   Available since

   Oct. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

7. FusX TALEN assembly system

   Plasmid Kit

   #1000000063

   Purpose

   Golden Gate assembly of 15-16 RVD TALEN inserts in a single cloning step; designed to be used with high throughput robotics

   Depositor

   Stephen Ekker

   Available since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

8. GoldenPiCS Kit

   Plasmid Kit

   #1000000133

   Purpose

   GoldenPiCS: plasmid kit for engineering in P. pastoris (GoldenMOCS based). Overexpression of up to eight transcription units. 20 promoters, 10 terminators, 4 selection markers and 4 integration sites

   Depositor

   Brigitte Gasser

   Available since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

9. CRISPR-Cas9 Gateway System for Physcomitrella patens

   Plasmid Kit

   #1000000151

   Purpose

   CRISPR-Cas9 Gateway System is a collection of 24 plasmids providing a modular, efficient CRISPR/Cas9 editing system for accurate and customizable repair in Physcomitrella patens.

   Depositor

   Magdalena Bezanilla

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

10. pCri System

    Plasmid Kit

    #1000000058

    Purpose

    pCri System: plasmids for cytoplasmic and periplasmic/extracellular heterologous protein expression in Escherichia coli, Bacillus subtilis, and Pichia pastoris.

    Depositor

    F Xavier Gomis-Ruth

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only