27,467 results
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Plasmid#66273PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEDNRA (EDNRA Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
CCR7-Tango
Plasmid#66244PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCCR7 (CCR7 Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
ADRA2B-Tango
Plasmid#66217PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertADRA2B (ADRA2B Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
PM2-FRB-CFP
Plasmid#67518PurposeFRB conjugated plasma membrane targeting sequences 2 from Lyn11(GCIKSKGKDSA)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmTOR (MTOR Human)
TagsCFP, HA, and PM tethering sequence from human Lyn…ExpressionMammalianMutationFRB domain only (residues 2019–2114)PromoterCMVAvailable sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
G protein alpha-s-GFP
Plasmid#66083PurposeG protein alpha-s internally tagged with EGFP and EE epitopeDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertG-alpha-s-EE-EGFP (Gnas Rat)
TagsEGFP flanked by SGGGS on each side was inserted i…ExpressionMammalianMutationBamHI sites in the alpha-s cDNA were removed and …PromoterCMVAvailable sinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
LSL
Plasmid#66590PurposeLox-STOP-Lox reporterDepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLSL-Tomato
Available sinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
mTagRFP-T-Endosomes-14
Plasmid#58014PurposeLocalization: Endosomes, Excitation: 555, Emission: 584DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEndosomes (RHOB Human)
TagsmTagRFPExpressionMammalianPromoterCMVAvailable sinceJuly 31, 2015AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
TPST1-EGFP
Plasmid#66617PurposeEncodes GFP fusion of TPST1 trans Golgi marker; (Tyrosyl protein sulfotransferase 2)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTPST1 (TPST1 Human)
ExpressionMammalianAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Str-KDEL_flGalT-SBP-EGFP
Plasmid#65272Purposesynchronize trafficking of GalT from the ER (RUSH system)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertgalactosidase
TagsEGFP and Streptavidin Binding Protein (SBP)ExpressionMammalianPromoterCMVAvailable sinceJune 19, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Str-KDEL_flGalT-SBP-mCherry
Plasmid#65273Purposesynchronize trafficking of GalT from the ER (RUSH system)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertgalactosidase
TagsStreptavidin Binding Protein (SBP) and mCherryExpressionMammalianPromoterCMVAvailable sinceJune 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
Mucolipin1-pHcRed C1
Plasmid#62959Purposefull length human Mucolipin-1 cloned into pHcRed1 C1DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMucolipin-1 (MCOLN1 Human)
TagsHcRed1ExpressionMammalianMutationMucolipin-1 has 1 silent mutation compared to the…PromoterCMVAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGuide
Plasmid#64711PurposeCloning and expression of Streptococcus pyogenes guide RNADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertStreptococcus pyogenes guide RNA
UseCRISPRExpressionMammalianPromoterhU6Available sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mTagGFP2-Clathrin-15
Plasmid#56413PurposeLocalization: Clathrin Vesicles, Excitation: 483, Emission: 506DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertClathrin (CLTB Human)
TagsmTagGFP2ExpressionMammalianPromoterCMVAvailable sinceApril 28, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
PM-ABKAR
Plasmid#61506PurposeExpression of AMPK biosensor targeted to the plasma membrane via Lyn kinase targeting sequenceDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAMPK Biosensor
TagsLyn kinase sequence (see comments)ExpressionMammalianPromoterCMVAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
ER-ABKAR
Plasmid#61508PurposeExpression of AMPK biosensor targeted to the endoplasmic reticulumDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAMPK Biosensor
TagsER targeting sequence from CYP450 2C1 (see commen…ExpressionMammalianPromoterCMVAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
KIF1A(1-393)-GCN4-2xmCherry-EF(C)
Plasmid#61665PurposeTandem mCherry-labeled, forced-dimeric, truncated kinesin-3 fused to EF Hand linkerDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertKIF1A(1-393)-GCN4-2xmCherry-EF(C) (KIF1A Human)
ExpressionMammalianAvailable sinceMarch 19, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAC-EHER
Plasmid#53262PurposeContains a 7 gene carotenoid pathway gene cluster of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces zeaxanthin diglucoside in Escherichia coliDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertcrtE, idi, crtX, crtY, crtI, crtB, crtZ
UseLow copy number bacterial cloning vectorPromoterendogenous promotersAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PA-mCherry1-Integrin-Beta1-N-18
Plasmid#57183PurposeLocalization: Integrin/Focal Adhesions, Excitation: 400 / 504, Emission: 515 / 517DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertIntegrin-Beta1 (ITGB1 Human)
TagsPA-mCherry1ExpressionMammalianPromoterCMVAvailable sinceJan. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDS24
Plasmid#58952PurposeEncodes synthetic mitochondrial gene ARG8m in place of COX3 coding sequence. Flanked by mtDNA sequences allowing integration into rho+ mtDNA after transformation.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMitochondrially coded Arg8 protein
MutationCoding sequence synthetic in S. cerevisiae mitoch…Promotern/aAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
mCherry-MannII-N-10
Plasmid#55074PurposeLocalization: Golgi Complex, Excitation: 587, Emission: 610DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMannII
TagsmCherryExpressionMammalianMutationaa 1-112 of MannIIPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by