27,462 results
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Plasmid#102246PurposeExpresses fusion of CRY2PHR with mCherry and Sec61BDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCRY2PHR-mCherry-SEC61beta (CRY2 Mustard Weed, Human)
ExpressionMammalianPromoterCMVAvailable sinceNov. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBItetDMPKSGFP
Plasmid#96903Purposetet inducible bidirectional plasmid expressing GFP in one direction and in the other a human DMPK genomic segment containing exons 11-15 with 0 CTG repeats in exon 15 locatedDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEGFP and human DMPK exons 11-15 with 0 CTG repeats (DMPK Human)
UseTetracycline responsive and bidirectionalExpressionMammalianPromotertetracycline responsive and bidirectionalAvailable sinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRK5 Flag-RagC(S75N)
Plasmid#99724PurposeMammalian expression of Flag-RagC(S75N)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRagC (RRAGC )
TagsFlagMutationS75NPromoterCMVAvailable sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-22:CETN2-mTagRFP-T
Plasmid#101781PurposeHomology arms and mTagRFPT-linker sequence for N-terminus tagging of human CETN2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCETN2 Homology Arms with mTagRFPT-linker (CETN2 Human)
UseCRISPR; Donor templateTagsmTagRFPT-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …Available sinceOct. 31, 2017AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pIRIGF-IKZF1-V2
Plasmid#69045Purposeexpression of IKZF1 fused to firefly luciferase with co-expression of Renilla luciferase, both under IRES control.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertIKZF1 (IKZF1 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationsplice variant 2PromoterIRESAvailable sinceOct. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
TRAIP-SFB
Plasmid#101769PurposeMammalian expression of TRAIP fusion proteinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTRAIP / RNF206 (TRAIP Human)
TagsS peptide-flag-streptavidin binding peptide (SFB)ExpressionMammalianMutationsiRNA-resistant alleleAvailable sinceOct. 16, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCLIPf-NK1R Control Plasmid
Plasmid#101125PurposeExpression of CLIP-tagged Neurokinin-1 Receptor in Mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNeurokinin-1 Receptor, Truncated (TACR1 Human)
Tags10xHis, CLIP-tag (CLIPf), and FLAG EpitopeExpressionMammalianPromoterCMVAvailable sinceSept. 27, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
ciCas9_pcDNA5
Plasmid#100550PurposeExpresses ciCas9 in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertciCas9
UseCRISPR; Frt, flp-inTagsFLAGExpressionMammalianAvailable sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG MCP-VP64
Plasmid#92357PurposeCAG-driven ubiquitous expression of MCP-VP64. MCP is bacteriophage MS2 coat protein, VP64 is transcriptional activation domain. Part of SAM-mediated gene activation system in chicken embryos.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMCP-VP64
UseCRISPRExpressionMammalianPromoterCAGAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCI Cas9 H2B-RFP
Plasmid#92397PurposeCAG-driven ubiquitous expression of Cas9 with Histone2B-RFP reporter controlled by IRESDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCas9
UseCRISPRExpressionMammalianPromoterCAGAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAcGFP-C2-hSphK2
Plasmid#84370PurposeExpression of AcGFP-SphK2 fusion protein in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSPHK2 (SPHK2 Human)
TagsAcGFPExpressionMammalianPromoterCMVAvailable sinceAug. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
UKNP1.18.1
Plasmid#98190PurposeExpression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHCV E1E2
ExpressionMammalianAvailable sinceAug. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
NRCoC
Plasmid#74300PurposeMammalian expression of full-length treacle fused to GFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserttreacle ribosome biogenesis factor 1 (Tcof1 Mouse)
TagsGFPExpressionMammalianPromoterCMVAvailable sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
sgRNA 1 GAPDH
Plasmid#83808PurposeExpress Sg-1 sgRNA targeting GAPDHDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSgRNA
UseCRISPRAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
MCS Lck only
Plasmid#90411PurposeA control of MCS+ that associated with cell plasma membrane only by one hydrophobic interaction at the N terminus of the FRET pair.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLck10+ Venus+mcherry
ExpressionMammalianAvailable sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX335-EN475
Plasmid#86231PurposeFor transient expression of spCas9-nickase and one sgRNA targeting the mouse CTCF locus. Use together with pX335-EN477.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertspCas9-nickase and sgRNA against mouse CTCF STOP Codon
UseMouse TargetingExpressionMammalianAvailable sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pZS2oxySp-GFP-proD-oxyR
Plasmid#78224Purposesynthetic gene circuitDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsynthetic gene circuit
Available sinceMay 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
Sirius-mT-Sapphire_pcDNA3
Plasmid#89584PurposeGenetically encoded ratiometric fluorescent thermometerDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSirius-mT-Sapphire
ExpressionMammalianPromoterCMVAvailable sinceMay 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLinker-AID-3xHA-HXGPRT-LoxP
Plasmid#86553Purposeused for a TIR1-AID system with CRISPR tagging technology in Toxoplasma gondiiDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLinker-AID-3xHA-HXGPRT 3'UTR
UseExpression in toxoplasma gondiiAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTagBFP-Akt1
Plasmid#86594PurposeExpresses TagBFP-tagged human Akt1(WT)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAkt1 (AKT1 Human)
ExpressionMammalianAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only