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32,870 results

Showing: 3221 - 3240 of 32870 results

  1. pET30a-His6-LOXCATmut

    Plasmid
    #140085
    Purpose
    A control for His6-LOXCAT where LOX is catalytically dead (mutations H265A and R268A)
    Depositor
    Vamsi Mootha
    Article
    Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103
    Insert
    A fusion of lactate oxidase from A. viridans and catalase from E. coli
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Mutation in H265A and R268A in LOX (used original…
    Promoter
    T7
    Available Since
    Sept. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    140085-plasmid-map-sequence-id-311165

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  2. pET30a-His6-ABP-LOXCAT

    Plasmid
    #139971
    Purpose
    Fusion of A. viridans lactate oxidase (LOX) and E. coli catalase (CAT) that converts lactate into pyruvate without producing H2O2. Contains a albumin binding peptide (ABP).
    Depositor
    Vamsi Mootha
    Article
    Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103
    Insert
    A fusion of lactate oxidase from A. viridans and catalase from E. coli
    Tags
    His6
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Sept. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    139971-plasmid-map-sequence-id-311161

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  7. pGBW-m4046955

    Plasmid
    #145616
    Purpose
    Bacterial Expression plasmid for RNA-dependent RNA polymerase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    RNA-dependent RNA polymerase (Nsp12) (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;FLAG_2
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145616-plasmid-map-sequence-id-307396

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  8. pMO9075

    Plasmid
    #117481
    Purpose
    empty complementation vector for Desulfovibrio: contains promoter from Aph(3')-IIa but no RBS, contains pBG1 and pMB1 replicons, SpecR
    Depositor
    Judy Wall
    Article
    Keller et al Methods Enzymol. 2011;497:503-17. doi: 1
    Insert
    Aph(3')-IIa promoter
    Expression
    Bacterial
    Promoter
    Aph(3')-IIa promoter
    Available Since
    Feb. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    117481-plasmid-map-sequence-id-273023

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  10. pHCL151

    Plasmid
    #134455
    Purpose
    TetR, lacI-Plac-ssdsbA-mscarN, Integrative plasmid for the expression of fully-screted prey. N-terminal mScarlet-I fusion.
    Depositor
    Thomas Bernhardt
    Article
    Lim et al Mol Microbiol. 2019 Sep 24. doi: 10.1111
    Insert
    linker-mScarlet-I for C-terminal fusion
    Expression
    Bacterial
    Promoter
    plac
    Available Since
    Jan. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    134455-plasmid-map-sequence-id-270547

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  12. pET-EIN-AlkBH5(66-292)

    Plasmid
    #125732
    Purpose
    Plasmid with EIN as a solubility and expression enhancing tag to increase production of hard to express proteins such as AlkBH5 and TEV cleaving site for the removal of the fusion tag.
    Depositor
    Vincenzo Venditti
    Article
    Khatiwada et al Protein Expr Purif. 2019 Nov 15;167:1055
    Insert
    AlkBH5(66-292) (ALKBH5 Human)
    Tags
    6xHis and EIN
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Nov. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    125732-plasmid-map-sequence-id-244619

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  13. K560GFP

    Plasmid
    #129761
    Purpose
    Expresses Drosophila Kinesin-1 truncated after coil 1 at 560 and tagged with GFP for single-molecule motor assays
    Depositor
    William Hancock
    Insert
    KHC (Khc Fly)
    Tags
    GFP and His6
    Expression
    Bacterial
    Mutation
    Truncation at 559
    Available Since
    Nov. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    129761-plasmid-map-sequence-id-422377

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  16. pCDF-5xT7-TtCsm

    Plasmid
    #128572
    Purpose
    For expression of T. thermophilus Csm in E. coli (Csm1-5)
    Depositor
    Jennifer Doudna
    Article
    Liu et al Nat Commun. 2019 Jul 5;10(1):3001. doi:
    Inserts
    Csm1
    Csm2, Csm3, Csm4, Csm5
    Tags
    HRV 3C protease cleavage site and 10xHis tag on C…
    Expression
    Bacterial
    Mutation
    Codon optimized for expression in E. coli and Cod…
    Promoter
    T7 promoter, lac operator
    Available Since
    Aug. 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    128572-plasmid-map-sequence-id-252577

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  17. pSLC-284

    Plasmid
    #124294
    Purpose
    A low copy plasmid constitutively expressing vsfGFP-9. This construct improves brightness of sfGFP by fusing a GFP-specific single domain antibody to it that creates a monomeric fluorophore.
    Depositor
    Swaine Chen
    Article
    Eshaghi et al Pathogens. 2016 Jan 5;5(1). pii: pathoge
    Insert
    vsfGFP-9
    Expression
    Bacterial
    Available Since
    July 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    124294-plasmid-map-sequence-id-251247

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Showing: 3221 - 3240 of 32870 results