30,424 results

1. pAMS823 His6-MBP-3C-ORF2p (238-1061, ORFeus-Hs) in pET41

   Plasmid

   #213024

   Purpose

   Expresses human LINE-1 ORF2p Core (238-1061) in E. coli as an N-MBP fusion

   Depositor

   Oliver Weichenrieder, Martin S. Taylor

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   LINE-1 ORF2p (238-1061)
   

   Use

   Tags

   His6-MBP-3C

   Expression

   Bacterial

   Mutation

   Promoter

   T7 / Lac

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pScI_dCas9-CDA_J23119-sgRNA

   Plasmid

   #108550

   Purpose

   Bacterial Target-AID vector with sgRNA

   Depositor

   Akihiko Kondo

   Article

   Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

   Inserts

   dCas9-PmCDA1
   Streptococcus pyogenes sgRNA
   

   Use

   Tags

   Flag

   Expression

   Bacterial

   Mutation

   D10A and H840A for SpCas9

   Promoter

   J23119 and lambda OR

   Available Since

   Sept. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX-4T1_ATG4B_BamHI_NotI

   Plasmid

   #190862

   Purpose

   Plasmid for the expression and purification of of ATG4B. Internal reference: SMC1392

   Depositor

   Sascha Martens

   Article

   Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

   Insert

   ATG4B (ATG4B Human)
   

   Use

   Tags

   GST, Thrombin Cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-Sfp R4-4 mutant

   Plasmid

   #173491

   Purpose

   Expression plasmid for mutant R4-4 of Sfp phosphopantetheinyl transferase. R4-4 can use 3′-dephospho CoA for adding the phosphopantetheinyl group to the carrier proteins.

   Depositor

   Jun Yin

   Article

   Sunbul et al J Mol Biol. 2009 Apr 10;387(4):883-98. d

   Insert

   Sfp-R44
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. A24M04 Ig kappa light chain-pFEEKW

   Plasmid

   #234810

   Purpose

   Express Ig kappa light chain containing the VL region of the monoclonal antibody, A24M04, specific for human papilloma virus16 L1

   Depositor

   Susan Pierce

   Article

   Torgbor et al Proc Natl Acad Sci U S A. 2025 Mar 11;12

   Insert

   A24M04 Igkappa light chain (IGKC, IGK Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   EEK promoter

   Available Since

   March 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX6P1-NEDD4L_FL

   Plasmid

   #187712

   Purpose

   Express GST-tagged NEDD4L FL in bacterial cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L FL (NEDD4L Human)
   

   Use

   Tags

   GST-HRV 3C site

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMVV109

   Plasmid

   #215339

   Purpose

   Expresses sfGFP under induction with the 3OC14-HSL Marionette Cassette (CinRAM + PCin-sfGFP) with a BBR1 backbone.

   Depositor

   Brian Pfleger

   Article

   Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

   Insert

   sfGFP
   

   Use

   Synthetic Biology

   Tags

   None

   Expression

   Bacterial

   Mutation

   Promoter

   PCin

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMSP1

   Plasmid

   #20060

   Depositor

   Stephen Sligar

   Article

   Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

   Insert

   MSP1 (APOA1 synthetic gene)
   

   Use

   Tags

   6-His

   Expression

   Bacterial

   Mutation

   synthetic gene of deletion mutant (1-43) of human…

   Promoter

   Available Since

   Jan. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET32a-LIC

   Plasmid

   #62310

   Purpose

   E. coli expression vector with N-terminal Trx-tag, 6X His-tag, thrombin site, S-tag, and TEV

   Depositor

   Cheryl Arrowsmith

   Type

   Empty backbone

   Use

   Tags

   6xHis, S-tag, and Trx-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lacO

   Available Since

   March 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCRISPR-PsppA-mCherry

    Plasmid

    #225428

    Purpose

    Plasmid encodes SgRNA, SpCas9 and homologous arms for homologous recombination of mCherry

    Depositor

    Geir Mathiesen

    Article

    Wiull et al Microbiol Spectr. 2025 Feb 4;13(2):e0202

    Insert

    mCherry, Cas9, SgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PsppA upstream of mCherry and Cas9, P3 upstream o…

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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