31,623 results
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Plasmid#167849PurposeBacterial expression of the SARS-CoV-2 proteins nsp10 and nsp16 from a bicistronic mRNADepositorInsertnsp10, nsp16
Tags6xHis-TEVExpressionBacterialPromoterT7Available SinceApril 22, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET24a-VHH-APEX2
Plasmid#109420PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody fused to ascorbate peroxidase 2. VHH-APEX2 also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, APEX2, HA, BAP and His6 epitope
TagsBAP, HA, His6, and T7ExpressionBacterialPromoterT7Available SinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMAL-SH3(2)-5R
Plasmid#112089PurposeBacterial expression plasmid containing His and MBP tags for 5 repeats of the second SH3 domain from human NCK1.DepositorInsertSH3(2)-5R (NCK1 Human)
TagsHis6 and MBPExpressionBacterialMutationconstruct contains only repeats of the second SH3…PromoterLacAvailable SinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
vGlut1 peptide- GST
Plasmid#218677PurposeExpresses a GST fusion to vGlut peptide for immunoisolation of glutamatergic vesiclesDepositorAvailable SinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
5VBD (USP09X)
Plasmid#101260PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceOct. 2, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMRS-XylS-dCas9
Plasmid#220196Purposemini-Tn7 dCas9 expression vector - XylS/pM promoterDepositorInsertPseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9
UseCRISPRExpressionBacterialMutationPseudomonas aeruginosa codon optimizedPromoterXylS/pMAvailable SinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEB2-mNeptune2.5
Plasmid#104013PurposePlasmid encoding mNeptune2.5DepositorInsertmNeptune2.5
UseLow copyExpressionBacterialMutationMutations relative to eqFP578 (MSKGEE LIKENM… M11…PromoterproCAvailable SinceDec. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTHSSe_49
Plasmid#109247PurposepSC101 var7 BbsI cloning vectorDepositorInsertsfGFP
ExpressionBacterialPromoterPJ23105Available SinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pGTVL2-CRBN-TBD
Plasmid#196369Purposeprotein expression of the GST-tagged Cereblon Thalidomide binding domainDepositorInsertCRBN (CRBN Human)
TagsHis6-GST-TEVExpressionBacterialMutationcontains only T319-T425PromoterT7Available SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pet42c(+)-GST-USP5
Plasmid#61750Purposeexpress N-terminally GST tagged human USP5DepositorAvailable SinceFeb. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1(5CNW)RS
Plasmid#222872PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 5-Cyano-L-Tryptophan (5-CN-Trp) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
ExpressionBacterialPromoterT7Available SinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
NylCA-TS_pET28a
Plasmid#215419PurposeExpression construct for NylCA-TS gene, codon optimized for expression in E. coliDepositorInsertNylCA-TS
ExpressionBacterialMutationS111G/A137L/E263QAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDDGFP-A
Plasmid#40286DepositorInsertddGFP-A
TagsHis-tagExpressionBacterialAvailable SinceSept. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET28a_T7-ARG2
Plasmid#106474PurposeExpresses acoustic reporter genes or gas vesicles (ARG2) in Escherichia coliDepositorInsertARG2
UseSynthetic BiologyExpressionBacterialPromoterT7Available SinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAJM.677
Plasmid#108530PurposeAraCAM + AraE + PBAD-YFP Reporter. Ara sensor for use in non-Marionette strain.DepositorInsertAraCAM + AraE + PBAD-YFP
ExpressionBacterialMutationAraC: L133F E165L E169G C280LAvailable SinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXT131_TwinStrep-SUMO-ShTniQ
Plasmid#135527PurposeProtein expression construct for ShTniQDepositorInsertShTniQ
TagsTwinStrep-SUMOExpressionBacterialAvailable SinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Nsp5_Mpro
Plasmid#156475PurposeBacterial expression of Sars-CoV2 Nsp5_Mpro protein with His-tag and GST-tagDepositorInsertNsp5_Mpro (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
TagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBB530
Plasmid#27391DepositorInsertgrpE
ExpressionBacterialAvailable SinceApril 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-T7-OmpA-MNaseA-6xHis
Plasmid#214808PurposeExpression of 6xhis-tagged micrococcal nuclease A (MNaseA)DepositorInsertOmpA-Micrococcal nuclease A
Tags6xhisExpressionBacterialPromoterT7 promoterAvailable SinceNov. 27, 2024AvailabilityAcademic Institutions and Nonprofits only