29,754 results

1. pHelper_TetR/pTet-Tnpase (carbR)

   Plasmid

   #213911

   Purpose

   ATc-inducible himar1C9 transposase expression - carb/amp resistance

   Depositor

   James Collins

   Article

   English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

   Insert

   TetR/ptet ATc-inducible himar1C9 transposase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDonor_Tn[sfGFP]

   Plasmid

   #213902

   Purpose

   Mariner transposon expressing sfGFP with kan selection casette

   Depositor

   James Collins

   Article

   English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

   Insert

   mariner transposon with kan resistance and superfolder GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET24c(+)-EFTu

   Plasmid

   #31172

   Depositor

   Linda Spremulli

   Article

   Zhang et al FEBS Lett. 1996 Aug 12. 391(3):330-2.

   Insert

   E. coli elongation factor Tu (tufB E. coli)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 30, 2011

   Availability

   Academic Institutions and Nonprofits only

4. pRK603

   Plasmid

   #8831

   Depositor

   David Waugh

   Article

   Kapust et al Protein Expr Purif. 2000 Jul . 19(2):312

   Insert

   TEV protease, S219D mutant
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   S219D mutant

   Promoter

   Available since

   June 22, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAT207

   Plasmid

   #134884

   Purpose

   Bacterial expression

   Depositor

   Thomas Howard

   Article

   Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

   Insert

   mRFP1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pENO1-dTom-NATr

   Plasmid

   #52208

   Purpose

   Constitutive expression of Candida codon-optimized dTomato with NAT selectable marker

   Depositor

   Robert Wheeler

   Article

   Gratacap et al Dis Model Mech. 2013 Sep;6(5):1260-70. d

   Insert

   dTomato
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   codon optimized for Candida species

   Promoter

   ENO1

   Available since

   March 24, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pET28a-PHGDH

   Plasmid

   #74113

   Purpose

   Expression of N-terminal His-tagged

   Depositor

   Lewis Cantley

   Article

   Mullarky et al Proc Natl Acad Sci U S A. 2016 Feb 16;11

   Insert

   PHGDH (PHGDH Human)
   

   Use

   Tags

   His-tagged

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUG-natNT2

   Plasmid

   #110922

   Purpose

   expression of nat gene conferring resistance to nourseothricin for gene deletion in yeast

   Depositor

   Jean-Marc Daran

   Article

   Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

   Insert

   nat
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   AgTEF1

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBAD/His-miRFP703

   Plasmid

   #79985

   Purpose

   Monomeric near-infrared fluorescent protein miRFP703 in bacterial plasmid

   Depositor

   Vladislav Verkhusha

   Article

   Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

   Insert

   miRFP703
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   araB

   Available since

   July 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Beta-Lactamase

    Plasmid

    #62729

    Purpose

    Expresses Beta-Lactamase protein in bacterial cells

    Depositor

    Niels Geijsen

    Article

    D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

    Insert

    Beta-Lactamase
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Deletion of periplasmic signal domain

    Promoter

    T7

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pPGC-K

    Plasmid

    #174581

    Purpose

    pOPIN-GG expression vector compatible with C-terminal tagging, Kanamycin resistant

    Depositor

    Mark Banfield

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. 6xHis-MBP-huPb2Cas12a

    Plasmid

    #174686

    Purpose

    Expresses 6xHis-MBP tagged Pb2Cas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huPb2Cas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET29_LuxSit_i

    Plasmid

    #194299

    Purpose

    Bacterial expression of the de novo luciferase, LuxSit-i

    Depositor

    David Baker, Andy Yeh

    Article

    Yeh et al Nature. 2023 Feb;614(7949):774-780. doi:

    Insert

    LuxSit-i
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGEX6p1-GST-Rnf31

    Plasmid

    #63134

    Purpose

    Expression of human RNF31/HOIP codon optimized for E. coli and insect cells

    Depositor

    Titia Sixma

    Article

    Smit et al EMBO J. 2012 Oct 3;31(19):3833-44. doi:

    Insert

    Rnf31 (RNF31 Human, Synthetic)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Synthetic based on human protein sequence

    Promoter

    tac

    Available since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

15. 4xNLS-pMJ915v2-sfGFP

    Plasmid

    #88921

    Purpose

    For expression of modified SpyCas9 in e.coli.

    Depositor

    Jennifer Doudna

    Article

    Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Inserts

    Cas9
    T7
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28a-AncMEcoGII250

    Plasmid

    #205025

    Purpose

    Expressing engineered M.EcoGII with higher thermostability

    Depositor

    Paola Laurino

    Article

    Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

    Insert

    Ancestral M.EcoGII 250
    

    Use

    Tags

    His6x tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET-24a-ELP[V-150]

    Plasmid

    #67015

    Purpose

    Expresses ELP[V-150] in E. coli

    Depositor

    Ashutosh Chilkoti

    Article

    Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

    Insert

    ELP[V-150]
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28a_MBP_RBFOX2

    Plasmid

    #224292

    Purpose

    MBP-RBFOX2 protein purification from bacteria

    Depositor

    Chuan He

    Article

    Dou et al Nat Cell Biol. 2023 Sep;25(9):1359-1368.

    Insert

    RNA binding fox-1 homolog 2 (RBFOX2 Human)
    

    Use

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Aug. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a-TRBP

    Plasmid

    #50351

    Purpose

    bacterial expression of TRBP

    Depositor

    Kumiko Ui-Tei

    Article

    Takahashi et al PLoS One. 2013 May 2;8(5):e63434. doi: 1

    Insert

    TRBP (TARBP2 Human)
    

    Use

    Tags

    His and Myc

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GST-p35

    Plasmid

    #24895

    Depositor

    Harish Pant

    Article

    Amin et al J Neurosci Res. 2002 Feb 1. 67(3):354-62

    Insert

    p35 (CDK5R1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Full-length p35

    Promoter

    Available since

    Feb. 16, 2011

    Availability

    Academic Institutions and Nonprofits only