32,401 results
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Plasmid#132610Purposeexpresses human RNF146 full length in E. coliDepositorInsertRNF146 (RNF146 Human)
ExpressionBacterialAvailable SinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
gZiPro
Plasmid#198441PurposeActivity/inhibition assays, 19F-NMRDepositorInsertglycine linked Zika virus NS2B-NS3
TagsHis6-TEVExpressionBacterialMutationR95*A,PromoterT7 promoterAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE80L TriCatcher-ELP
Plasmid#112630PurposeTwo SpyCatchers linked by an elastin-like polypeptide with a C-terminal SnoopCatcherDepositorInsertTriCatcher-ELP
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT5Available SinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSB_init-Sb44
Plasmid#159421PurposeExpression of the anti-TGP (thermostable GFP) synthetic nanobody Sb44DepositorInsertSb44
Tagsc-Myc and poly-HisExpressionBacterialAvailable SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
MT2a
Plasmid#105693PurposeBacterial expression plasmids for production of recombinant metallothionein 2aDepositorAvailable SinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
DJ-1 TEV site pET15b
Plasmid#60687PurposeRecombinant protein expression of Park7DepositorInsertDJ-1 (PARK7 Human)
Tags6xHisExpressionBacterialMutationTEV site was inserted (see comments below).PromoterT7Available SinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
BARD1 26-327 pET28a
Plasmid#12646DepositorInsertBRCA1 binding partner for a Ring domain E3 Ub-ligase (BARD1 Human)
ExpressionBacterialAvailable SinceJuly 31, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGMCK-T38S38-750-luxAB-DAS+4
Plasmid#49518PurposeKanamycin resistant; expresses luxAB-DAS+4 under the control of a promoter containing PtetO-4C5G; constitutively expresses reverse TetR (tetR38); integrates into the mycobacteriophage att-L5 siteDepositorInsertT38S8-P750-luxAB-DAS+4
TagsDAS+4 (AANDENYSENYADAS)ExpressionBacterialPromoterP750Available SinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJMP1055
Plasmid#119242Purposeconstruction intermediateDepositorInsertsgRNA NT1/RR1 (rfp)
ExpressionBacterialAvailable SinceDec. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
T7-AglC-His6
Plasmid#89726PurposeExpresses AglC from M. voltae with an N-terminal T7 tag and a C-terminal His6 tag. Plasmid has codons optimized for E. coli expression.DepositorInsertAglC
TagsHis6 and T7ExpressionBacterialAvailable SinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
p006-GFP-strong
Plasmid#108313PurposeGFP from jellyfish Aquorea sp. under strong synthetic constitutive promoter pFAB4026; kanamycin resistant.DepositorInsertGreen fluorescent protein
ExpressionBacterialPromoterpFAB4026Available SinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT7-7 asyn S129A
Plasmid#36048DepositorAvailable SinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBLADE(FP6**)-mCherry
Plasmid#168049PurposeExpresses the synthetic gene BLADE FP6 under the constitutive J23101** promoter. mCherry expression is controlled by the PBAD promoter.DepositorInsertsBlue light-inducible AraC dimers in Escherichia coli
mCherry
ExpressionBacterialPromoterJ23101** and PBADAvailable SinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
HA-HA-AR-DBD-LBD
Plasmid#171237PurposeMammalian expression of 2xHA-tagged human androgen receptor trancate: AR DNA-bindind domain and Ligand-binding domain (DBD-LBD)DepositorInsert2xHA-tagged human androgen receptor trancate:DNA-binding domain and Ligand-binding domain (AR Human)
TagsHAExpressionBacterial and MammalianMutationAR truncatePromoterCMVAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTDpelB-C_sfYFPTwinStrep
Plasmid#45944Purposeexpresses P.putida codon optimized PelB-sfYFP-Twin-Strep fusion protein for periplasmic translocation via PelB signal sequenceDepositorInsertsynthetic sfYFP (codon usage adapted to P.putida KT2440)
TagsPelB export sequence and Twin-Strep-tagExpressionBacterialPromoterlacIq/PtrcAvailable SinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET His6 MBP prescission LIC cloning vector (HMPKS)
Plasmid#29721DepositorTypeEmpty backboneTags6xHis, MBP, and Prescission cleavage siteExpressionBacterialPromoterT7-lacO (lactose/IPTG inducible)Available SinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEM-Cas9HF1-recA56
Plasmid#89962PurposeModified from pEM-Cas9HF1 (Addgene ID: 89961) to include constitutive recA56 to block recA-mediated double-strand break repair.DepositorInsertsCas9HF1
recA56
ExpressionBacterialMutationN497A/R661A/Q695A/Q926APromoterpTet and proDAvailable SinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKD184
Plasmid#90953PurposeExpresses ThsR under the PltetO-1 promoter, sfGFP under PphsA, and mCherry constitutivelyDepositorInsertsThiosulfate response regulator
Superfolder GFP
mCherry
ExpressionBacterialPromoterBba_J23114, PltetO-1, and PphsAAvailable SinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
R702-X31-566: His6-MBP-tev-G-Hs.PDE6D(2-150)
Plasmid#159689PurposeE. coli protein expression of His6-MBP-tev-G-Hs.PDE6D(2-150)DepositorAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1(6/7FTrp)RS
Plasmid#207621PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 6-Fluoro-L-Tryptophan (6FTrp) and 7-Fluoro-L-Tryptophan (7FTrp) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
ExpressionBacterialPromoterT7Available SinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only