30,513 results

1. pFCcGi

   Plasmid

   #59324

   Purpose

   Inducible expression of GFP and constitutive expression of mCherry

   Depositor

   Sophie Helaine, David Holden

   Article

   Figueira et al MBio. 2013 Apr 16;4(2):e00065. doi: 10.1

   Inserts

   mCherry
   EGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pbad and rpsM

   Available Since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. SPLICSFAST-NU-MT-P2A

   Plasmid

   #214898

   Purpose

   Expresses equimolar Nucleus- and Mitochondria- targeted split-FAST fragments for Short-range NU-MT Contact sites

   Depositor

   Tito Calì

   Insert

   SPLICSFAST-NU-MT-P2A
   

   Use

   Tags

   none

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. SpySwitch

   Plasmid

   #184225

   Purpose

   Expresses SpySwitch in the bacterial cytoplasm. SpySwitch binds SpyTag-, SpyTag002- or SpyTag003-fusions non-covalently for affinity purification, allowing gentle pH or temperature release.

   Depositor

   Mark Howarth

   Article

   Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:

   Insert

   SpySwitch
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   E77A mutation prevents isopeptide bond formation,…

   Promoter

   T7

   Available Since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBroad-T7RNAP-chlorR

   Plasmid

   #199992

   Purpose

   T7 RNAP circuit variant T15 on ultra broad host range vector (RSF1010 and pAMB1 origins) chlorR

   Depositor

   Farren Isaacs

   Article

   Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

   Insert

   T7RNAP Circuit
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   copFnull

   Promoter

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET24a-VHH-APEX2

   Plasmid

   #109420

   Purpose

   Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to ascorbate peroxidase 2. VHH-APEX2 also contains a T7, HA, BAP and His6 epitope

   Depositor

   Martin Spiess

   Article

   Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

   Insert

   anti-GFP nanobody fused to a T7, APEX2, HA, BAP and His6 epitope
   

   Use

   Tags

   BAP, HA, His6, and T7

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-30a-L6KD-PT linker-PEP-Mtu ΔI-CM-xylanase

   Plasmid

   #231128

   Purpose

   Expresses xylanase via a cSAT2.0 scheme

   Depositor

   Xiaofeng Yang

   Article

   Huang et al Trends Biotechnol. 2024 Nov 12:S0167-779

   Insert

   xylanase
   

   Use

   Tags

   cSAT2.0

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Jan. 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGT-Ah1

   Plasmid

   #122568

   Purpose

   MAGIC donor plasmid (Himar transposon with beta-lactamase/sfGFP payload)

   Depositor

   Harris Wang

   Article

   Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

   Insert

   beta-lactamase/sfGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. HA-HA-AR-NTD-DBD

   Plasmid

   #171236

   Purpose

   Mammalian expression of 2xHA-tagged human androgen receptor trancate: AR N-terminal-domain and DNA-bindind domain (NTD-DBD)

   Depositor

   Steven Balk

   Article

   Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

   Insert

   2xHA-tagged human androgen receptor trancate:N-terminal-domain and DNA-binding domain (AR Human)
   

   Use

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Mutation

   AR truncate

   Promoter

   CMV

   Available Since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. His6-EGFP-PH-PLCD1

   Plasmid

   #183675

   Purpose

   PtdIns(4,5)P2 biosensor for purification from bacteria

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   PLCD1 (PLCD1 Human)
   

   Use

   Tags

   His6-EGFP

   Expression

   Bacterial

   Mutation

   Amino acids 11-140

   Promoter

   T7

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEM-Sox2

    Plasmid

    #16353

    Depositor

    James Thomson

    Article

    Yu et al Science. 2007 Dec 21. 318(5858):1917-20.

    Insert

    Homo sapiens SRY (sex determining region Y)-box 2 (SOX2) (SOX2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 4, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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