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pBbS2c-RFP

Plasmid

#35329

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

Tet

Available Since

Feb. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pUC-fFuCas9-HTBNLS-hph

Plasmid

#121092

Purpose

Multigene editing in the Fusarium fujikuroi genome using the CRISPR-Cas9 system

Depositor

Xiao-Jun Ji

Article

Shi et al ACS Synth Biol. 2019 Feb 15;8(2):445-454

Insert

Cas9 and hygromycin resist gene

Use

CRISPR

Expression

Bacterial

Promoter

gpdA;trpC

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pBAD-FimH-9xHis

Plasmid

#97305

Purpose

E.coli UTI89 FimH signal peptide and lectin domain

Depositor

Per Svenningsen

Insert

Type 1 fimbiral adhesin FimH

Tags

9xHis

Expression

Bacterial

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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ACOT4A

Plasmid

#42449

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

ACOT4A (ACOT4 Human)

Tags

His6-TEV

Expression

Bacterial

Mutation

See Comments

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pYTK003

Plasmid

#65110

Purpose

Encodes ConL1 as a Type 1 part to be used in the Dueber YTK system

Depositor

John Dueber

Article

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

ConL1

Expression

Bacterial

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pQIq_MRGS_HIS8_(27F3)_FLAG

Plasmid

#199615

Purpose

Encodes DARPin-FLAG 27F3 for bacterial expression and column purification

Depositor

Shiva Tyagarajan

Article

Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

Insert

DARPin-FLAG 27F3

Tags

8xHis and FLAG

Expression

Bacterial

Promoter

T5

Available Since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

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pDule- Mj Acd A9

Plasmid

#197651

Purpose

E. coli machinery vector for the genetic encoding of acridone at TAG codons using the M. jannaschii Acd-A9 synthetase/tRNA pair

Depositor

Ryan Mehl

Article

Sungwienwong et al Org Biomol Chem. 2017 May 3;15(17):3603-

Inserts

Mj Acd A9 synthetase
Methanocaldococcus jannaschii tRNA

Tags

None

Expression

Bacterial

Mutation

Y32A, L65D, F108L, Q109E, D158S, L162T

Promoter

GlnS and lpp

Available Since

April 6, 2023

Availability

Academic Institutions and Nonprofits only

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pHis APEX2-eIF4A1

Plasmid

#129645

Purpose

Express APEX2 fusion to DEAD-box translation factor eIF4A1 in bacteria

Depositor

Nicholas Ingolia

Article

Padron et al Mol Cell. 2019 Aug 22;75(4):875-887.e5.

Insert

eukaryotic translation initiation factor 4A1 (EIF4A1 Human)

Tags

6xHis and APEX2

Expression

Bacterial

Available Since

Sept. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pL0M-S-mTurquoise2-EC18152

Plasmid

#137074

Purpose

Golden Gate Level 0 S module for N-terminal fluorescent protein tagging

Depositor

Julian Hibberd

Article

Luginbuehl et al Plant Direct. 2020 Feb 11;4(2):e00188. d

Insert

mTurquoise2 DNA synthesis product with 5' and 3' extensions for Golden Gate cloning

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2020

Availability

Academic Institutions and Nonprofits only

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pET-3c-rhVTN-NC

Plasmid

#30226

Depositor

James Thomson

Article

Chen et al Nat Methods. 2011 May;8(5):424-9. doi: 1

Insert

rhVitronectin with N and C terminus cleavages (VTN Human)

Expression

Bacterial

Mutation

deleted amino acids: 399-478, 20-62

Available Since

Sept. 26, 2011

Availability

Academic Institutions and Nonprofits only

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pBL090J

Plasmid

#195045

Purpose

cCadC-CCB3.5 under the control of PBAD

Depositor

Tina Wang

Article

Lee et al ACS Synth Biol. 2022 Oct 21;11(10):3198-

Insert

cCadC-CCB3.5

Expression

Bacterial

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaM-6

Plasmid

#172771

Purpose

Bacterial expression of anti-mCherry nanobody LaM-6, with pelB leader and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaM-6

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pLAM12

Plasmid

#26908

Depositor

Graham Hatfull

Article

van Kessel et al Nat Methods. 2007 Feb . 4(2):147-52.

Insert

Acetamidase Promoter

Expression

Bacterial

Available Since

Feb. 3, 2011

Availability

Academic Institutions and Nonprofits only
pGEX6P1-mClover3-anti-rabbit-IgG(Fc)-nanobody

Plasmid

#138129

Purpose

Expresses GST-tagged mClover3-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

Insert

mClover3-anti-rabbit-IgG(Fc)-nanobody

Tags

GST

Expression

Bacterial

Available Since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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GB1-A11(2-196, G38R)-Strep

Plasmid

#196209

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, G38R)-Strep.

Depositor

Lalit Deshmukh

Article

Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

Insert

Annexin A11 (ANXA11 Human)

Tags

GB1-6xHis-TEV and Strep-II tag

Expression

Bacterial

Mutation

G38R

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-(SUMO)3

Plasmid

#127093

Purpose

Bacterial expression plasmid for eGFP-(SUMO)3 client to assess SUMO-SIM scaffolds.

Depositor

Michael Rosen

Article

Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

Insert

EGFP - 3 repeats of human SUMO3 each separated by (GGS)4 (SUMO3 Human)

Tags

EGFP

Expression

Bacterial

Promoter

unknown

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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Ecoli cpGFP

Plasmid

#215713

Purpose

Expresses cpGFP in Ecoli

Depositor

Katharine Diehl

Article

Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

Insert

cpGFP

Tags

6xHis

Expression

Bacterial

Mutation

circularly permuted GFP

Promoter

T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pET_RGG-GFP-RGG

Plasmid

#124948

Purpose

Expression of RGG-GFP-RGG

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Tags

EGFP and His

Expression

Bacterial

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0279

Plasmid

#149688

Purpose

Bacterial expression of SENP_Eu protease test substrate H14-SUMO_Eu1-MBP-3xFlagTag

Depositor

Dirk Görlich

Article

Vera Rodriguez et al J Cell Biol. 2019 Jun 3;218(6):2006-2020

Insert

SUMO_Eu1-MBP

Tags

3xFLAG-tag and His14-tag

Expression

Bacterial

Available Since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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pCold TDP43 Q331K

Plasmid

#27454

Depositor

Aaron Gitler

Article

Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

Insert

TDP-43 (TARDBP Human)

Tags

Factor Xa and His6

Expression

Bacterial

Mutation

Glutamine 331 mutated to Lysine (Q331K)

Available Since

Feb. 10, 2011

Availability

Academic Institutions and Nonprofits only