30,756 results

1. pTD-NStrepHis

   Plasmid

   #45936

   Purpose

   use to create N-terminal Strep-tagII fusion proteins or dual tagged fusion with N-terminal Strep-tagII and C-terminal 6x His-tag

   Depositor

   Thorben Dammeyer

   Article

   Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

   Type

   Empty backbone

   Tags

   6xHis and Strep II

   Expression

   Bacterial

   Promoter

   Trc (lactose/IPTG inducible)

   Available Since

   Sept. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET26b-Granzyme A

   Plasmid

   #8823

   Depositor

   Judy Lieberman

   Article

   Beresford et al Proc Natl Acad Sci U S A 1997 Aug 19;94(

   Insert

   Human Granzyme A (GZMA Human)
   

   Tags

   (His)6 and Enterokinase site

   Expression

   Bacterial

   Mutation

   it mature enzyme.

   Available Since

   Sept. 26, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-2(KW)2-eGFP

   Plasmid

   #112736

   Purpose

   Express 2(KW)2-eGFP in E. coli

   Depositor

   Kyubong Jo

   Article

   Lee et al Nucleic Acids Res. 2016 Jan 8;44(1):e6.

   Insert

   2(KW)2-eGFP
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7 Promoter

   Available Since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUM

   Plasmid

   #64176

   Purpose

   harbours marker genes URA3 and MET17

   Depositor

   Markus Ralser

   Article

   Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

   Type

   Empty backbone

   Expression

   Bacterial and Yeast

   Available Since

   Aug. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. Parp14-c010

   Plasmid

   #173100

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   PARP14 (PARP14 Human)
   

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   F1208-G1388

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRSETA-PAGFP

   Plasmid

   #11911

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Patterson et al Science. 2002 Sep 13. 297(5588):1873-7.

   Insert

   pRSETA-PAGFP
   

   Expression

   Bacterial

   Mutation

   The PAGFP has four mutations (L64F/T65S/V163A/T20…

   Available Since

   July 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRSET-QNA

   Plasmid

   #129305

   Purpose

   Negative control for ATP measurement

   Depositor

   Hiroyuki Noji

   Article

   Yaginuma et al Sci Rep. 2014 Oct 6;4:6522. doi: 10.1038

   Insert

   QUEEN-NA
   

   Expression

   Bacterial

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMT316

   Plasmid

   #8608

   Depositor

   Robert Hartley

   Article

   Hartley et al J Mol Biol 1988 Aug 20;202(4):913-5.

   Insert

   barstar
   

   Expression

   Bacterial

   Available Since

   Jan. 17, 2007

   Availability

   Academic Institutions and Nonprofits only

9. pKEW302-AcrVA4

   Plasmid

   #115656

   Purpose

   Expresses AcrVA4 in bacteria, under TetR control.

   Depositor

   Jennifer Doudna

   Article

   Watters et al Science. 2018 Oct 12;362(6411):236-239.

   Insert

   AcrVA4
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   Ptet

   Available Since

   Feb. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pWZ699

    Plasmid

    #208117

    Purpose

    Payload yeast assembly vector with TK-mActb on backbone

    Depositor

    Jef Boeke

    Article

    Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-

    Type

    Empty backbone

    Use

    Synthetic Biology; Copy number inducible

    Expression

    Bacterial, Mammalian, and Y…

    Promoter

    Human PGK1 for thymidine kinase

    Available Since

    Nov. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. O-TREM2-2

    Plasmid

    #166545

    Purpose

    scFv of a human scaffold targeting Triggering receptor expressed on myeloid cells 2. Antigen coverage aa 19-131 of 230

    Depositor

    Susanne Gräslund

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, His6 and OmpA leader sequence

    Expression

    Bacterial

    Promoter

    LacZ

    Available Since

    April 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTKDP-dhfr

    Plasmid

    #71323

    Purpose

    Donor plasmid - trimethoprim resistant donor cassette

    Depositor

    Thomas Kuhlman

    Article

    Tas et al PLoS One. 2015 Sep 2;10(9):e0136963. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. [pSK122] pETDuet GST-p18(delta5)

    Plasmid

    #136144

    Purpose

    bacterial expression of GST-p18

    Depositor

    David Sabatini, Kuang Shen

    Article

    Shen et al Proc Natl Acad Sci U S A. 2018 Sep 18;11

    Insert

    GST-p18
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a-6xHis-MBP-TEV site-TadA8e

    Plasmid

    #208319

    Purpose

    Overxpression MBP-TadA8e

    Depositor

    Weixin Tang

    Article

    Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    TadA8e
    

    Expression

    Bacterial

    Mutation

    mutations in TadA are described in the paper

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAB061i3

    Plasmid

    #79230

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    Ppro1 rpoZ; PlacIq lacI
    

    Expression

    Bacterial

    Promoter

    Ppro1; PlacIq

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRAM18dSGK[MCS]

    Plasmid

    #84684

    Purpose

    Can be used for the transformation of rickettsia

    Depositor

    Ulrike Munderloh

    Article

    Burkhardt et al PLoS One. 2011;6(12):e29511. doi: 10.137

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAN4036

    Plasmid

    #74698

    Purpose

    Output plasmid: PPhlF-PAmtR-YFP

    Depositor

    Christopher Voigt

    Article

    Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

    Insert

    PPhlF-PBetI-YFP
    

    Expression

    Bacterial

    Available Since

    May 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX SH3(2)-4R

    Plasmid

    #112090

    Purpose

    Bacterial expression plasmid containing a GST tag with 4 repeats of the second SH3 domain from human NCK1.

    Depositor

    Michael Rosen

    Article

    Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

    Insert

    SH3(2)-4R (NCK1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    Lac

    Available Since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKDsgRNA-rssA

    Plasmid

    #89957

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting rssA.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    rssA gRNA
    

    Expression

    Bacterial

    Promoter

    pTet

    Available Since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pETM6-H10-mCherry

    Plasmid

    #66531

    Purpose

    Expresses mCherry under the control of Mutant T7 Promoter H10

    Depositor

    Mattheos Koffas

    Article

    Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

    Insert

    mCherry
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Codon Optimized for E. coli

    Promoter

    Mutant T7 Promoter - H10

    Available Since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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