31,059 results
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Plasmid#208742PurposeCharacterization plasmid for PPgk (sfgfp expressed from PPgk on the S. aureus pC194 replicon)DepositorInsertPPgk
UseSynthetic BiologyExpressionBacterialPromoterPPgkAvailable SinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM33_CRK_SH3
Plasmid#178472PurposeBacterial expression of human domain with His-tag and GST tagDepositorAvailable SinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
GST-PS-Gads 1-155 GB1 Gads 261-330-Cys (XSB365)
Plasmid#78507PurposeConstruct for expressing Gads SH3, SH2, and SH3 domain in E. Coli, with a C-terminal Cys for labelingDepositorInsertGads SH3 SH2 SH3 domain (GRAP2 Human)
TagsGST followed by a prescission protease claevage s…ExpressionBacterialAvailable SinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCold TDP43 Q331K
Plasmid#27454DepositorInsertTDP-43 (TARDBP Human)
TagsFactor Xa and His6ExpressionBacterialMutationGlutamine 331 mutated to Lysine (Q331K)Available SinceFeb. 10, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHL 1405
Plasmid#53010PurposepLtetO-1:RhyB, pCon:DsrA, pLtetO-1:hfq, pLlacO-1:sodB::gfpDepositorInsertsRhyB sRNA
DsrA sRNA
hfq
sodB
UseSynthetic BiologyTagsgfpExpressionBacterialMutationcontains -56 to +141 relative to start codonPromoterpCon, pLlacO-1, and pLtetO-1Available SinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSUM-Kan-MCS2-gfp
Plasmid#109290PurposeExpresses high levels of gfp in mycobacteriaDepositorInsertGFP
ExpressionBacterialMutationWTPromoterKanamycinAvailable SinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDA303
Plasmid#195199PurposeOverexpressing 6His-TetR in E. coli.DepositorInsertTetR
Tags6xHisExpressionBacterialPromoterT5 promoterAvailable SinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAV0279
Plasmid#149688PurposeBacterial expression of SENP_Eu protease test substrate H14-SUMO_Eu1-MBP-3xFlagTagDepositorInsertSUMO_Eu1-MBP
Tags3xFLAG-tag and His14-tagExpressionBacterialAvailable SinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T moesin 1-577
Plasmid#11637DepositorAvailable SinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T1-Opt WWP1
Plasmid#179327PurposeE. coli optimized expression of GST-fusion of human WWP1DepositorInsertWWP1 (WWP1 Human)
TagsGSTExpressionBacterialMutationCodon optimized for expression in E. coliAvailable SinceApril 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-DAXX-ΔD/E-His
Plasmid#179618PurposeExpress GST-DAXX-ΔDE-His in bacteriaDepositorInsertdeath domain associated protein (DAXX Human)
TagsHisExpressionBacterialMutationdeleted amino acids 424-495Available SinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNCST-AvicFP2
Plasmid#129505PurposeExpresses AvicFP2 constitutively in E. coli (most strains)DepositorInsertAvicFP2
ExpressionBacterialPromotersynthetic constitutive (stationary phase) promote…Available SinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-mClover3-anti-rabbit-IgG(Fc)-nanobody
Plasmid#138129PurposeExpresses GST-tagged mClover3-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.DepositorInsertmClover3-anti-rabbit-IgG(Fc)-nanobody
TagsGSTExpressionBacterialAvailable SinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMRE135
Plasmid#118489Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertmScarlet-I
ExpressionBacterialAvailable SinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SnoopTagJr-MBP
Plasmid#105628PurposePlasmid for recombinant expression in bacteria of MBP fused to SnoopTagJr, permitting covalent linkage to DogTag by SnoopLigaseDepositorInsertSnoopTagJr-MBP
ExpressionBacterialPromoterT7Available SinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
GCN5L2
Plasmid#25482PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 23, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
AsCpf1-2NLS
Plasmid#102565Purposebacterial expression of AsCpf1-2NLSDepositorInsertAsCpf1
Tags6xHis-MBP-TEV and HA-2xSV40NLSExpressionBacterialPromoterT7Available SinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET32a-HD16Q
Plasmid#11487DepositorInserthuntingtin, exon 1, 16 glutamines (HTT Human)
TagsHis, His (out of frame), and TrxExpressionBacterialMutationhuntingtin exon 1 with 16 glutamines, vector m…Available SinceApril 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
pQFT
Plasmid#203674PurposepQF derivative with promoter PQ5 exchanged for promoter PQJDepositorTypeEmpty backboneTags3xFLAGExpressionBacterialPromoterPQJAvailable SinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAB082a
Plasmid#79233PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPpro1 rpoZ-Cry1Ac-d123
ExpressionBacterialPromoterPpro1Available SinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only