30,260 results

1. pET26b_Nb.AD101

   Plasmid

   #131402

   Purpose

   E. coli periplasmic expression of conformational nanobody Nb.AD101 recognizing purified active-state A2a adenosine receptor

   Depositor

   Andrew Kruse

   Article

   McMahon et al Nat Struct Mol Biol. 2018 Mar;25(3):289-

   Insert

   Nanobody AD101
   

   Use

   Tags

   6xHis-tag and pelB leader

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTriEx-mCherry-LOV2 I427T

   Plasmid

   #81035

   Purpose

   Mammalian expression of LOV I427T, a mutant with super short relaxation time

   Depositor

   Klaus Hahn

   Article

   Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

   Insert

   LOV I427T
   

   Use

   Tags

   mCherry

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   I427T

   Promoter

   Available since

   Aug. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET16_Caspase-4_p10

   Plasmid

   #214308

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   CASP4 (CASP4 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28_Caspase-4_p20_C258A

   Plasmid

   #214307

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   CASP4 (CASP4 Human)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   C258A

   Promoter

   T7

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBS human c-Myc

   Plasmid

   #14971

   Depositor

   Joan Massague

   Article

   Siegel et al J Biol Chem. 2003 Sep 12. 278(37):35444-

   Insert

   c-Myc (MYC Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 17, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBXCA3H

   Plasmid

   #47072

   Purpose

   FX cloning E.coli expression vector with araBAD promoter and C-terminal Avi-tag, 3C protease site and 10x His tag

   Depositor

   Raimund Dutzler, Eric Geertsma

   Article

   Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

   Type

   Empty backbone

   Use

   Tags

   3C protease site (PreScission site), Avi-tag, and…

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   Jan. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBS cPitx2 (CT#73)

   Plasmid

   #13927

   Depositor

   Cliff Tabin

   Article

   Logan et al Cell. 1998 Aug 7. 94(3):307-17.

   Insert

   pitx2 (PITX2 Chicken)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 9, 2007

   Availability

   Academic Institutions and Nonprofits only

8. pET28a-mH6-Cas12g1

   Plasmid

   #120879

   Purpose

   Expresses E. coli codon optimized Cas12g1 effector in the pET28a backbone.

   Depositor

   Arbor Biotechnologies

   Article

   Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

   Insert

   Cas12g1
   

   Use

   CRISPR

   Tags

   mH6

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET51b(+)_ALFA-tag-SNAP

   Plasmid

   #136628

   Purpose

   Plasmid encoding the bacterial expression of ALFA-tag asa SNAP-tag fusion

   Depositor

   Kai Johnsson

   Article

   Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

   Insert

   EGFP-NbALFA
   

   Use

   Tags

   Hisx10 and Strep

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. ZP46 Prickle-2

    Plasmid

    #16896

    Depositor

    Randall Moon

    Article

    Veeman et al Curr Biol. 2003 Apr 15. 13(8):680-5.

    Insert

    Prickle-2 (prickle2b Zebrafish)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

11. pCN38-yfp

    Plasmid

    #227637

    Purpose

    Negative control plasmid encoding yfp downstream of an attenuated ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and chloramphenicol resistance to S. aureus.

    Depositor

    Angelika Grundling

    Article

    Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

    Insert

    yfp
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. MP-P

    Plasmid

    #69661

    Purpose

    Untargetted Bacterial Mutagenesis

    Depositor

    David Liu

    Article

    Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

    Insert

    dnaQ926 dam seqA emrR mutH(E56A)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    E. coli PBAD

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX Src-SH2

    Plasmid

    #46510

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Src (SRC Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 142-252

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. FLI1_pet28a

    Plasmid

    #131641

    Purpose

    Bacterial expression of ETS gene FLI1

    Depositor

    Peter Hollenhorst

    Article

    Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

    Insert

    FLI1 (FLI1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAC-Kan-alphaGal4

    Plasmid

    #13421

    Depositor

    Keith Joung

    Article

    Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

    Insert

    RNAP alpha-Gal4
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. SPS-7942

    Plasmid

    #80459

    Purpose

    IPTG inducible expressoin of sucrose phosphate synthase derived from Synechococcus elongatus PCC 7942

    Depositor

    Daniel Ducat

    Article

    Abramson et al Plant Cell Physiol. 2016 Oct 13. pii: pc

    Insert

    Sucrose phosphate synthase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMB1-A-TelR

    Plasmid

    #204979

    Purpose

    Level 1 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains

    Depositor

    Veronica Godoy-Carter

    Article

    Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

    Insert

    mRFP1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Added replications elements for Acinetobacter spe…

    Promoter

    Available since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCS2TAL3-DD

    Plasmid

    #37275

    Purpose

    TALEN backbone vector for use with the Golden Gate TALEN Kit in place of pTAL1-4. Shorter N- and C-terminal tal protein segments increases mutation induction; Fok I DD (improved version=pCS2TAL3-DDD)

    Depositor

    David Grunwald

    Article

    Dahlem et al PLoS Genet. 2012 Aug;8(8):e1002861. Epub

    Type

    Empty backbone

    Use

    Tags

    Flag, Fok I DD, Tal-C, and Tal-N

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. GST-beta-arrestin2

    Plasmid

    #36919

    Depositor

    Robert Lefkowitz

    Article

    Xiao et al J Biol Chem. 2004 Dec 31;279(53):55744-5

    Insert

    beta-arrestin2 (Arrb2 Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 27, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pNM58

    Plasmid

    #128409

    Purpose

    System with AIP and xylose inducible agrCA sensor

    Depositor

    Christopher Voigt

    Article

    Gonzalez et al Nat Chem Biol. 2019 Dec 2. pii: 10.1038/

    Inserts

    argCA
    gfpmut2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P3 and PxylR

    Available since

    Sept. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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