30,804 results

1. pALS3-Ma-sfGFP-TAG150

   Plasmid

   #212121

   Purpose

   Fluorescence plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-TAG150 and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.

   Depositor

   Ryan Mehl

   Inserts

   sfGFP-150TAG
   M. alvus Pyl-tRNA(6)
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   N150TAG

   Promoter

   araC and lpp

   Available Since

   Sept. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBK-Ma pylRS nitroY/haloY-F5

   Plasmid

   #212125

   Purpose

   Expresses Methanomethylophilus alvus pyrrolysine tRNA synthetase engineered for 3-nitroY and 3-haloY under GlnS promoter. Used as a control for Ma Pyl tRNA-synthetase selections.

   Depositor

   Ryan Mehl

   Insert

   M. alvus PylRS 3-NY/HaloY F5
   

   Expression

   Bacterial

   Mutation

   CTG125CTT, N166A V168S, A223C, W239R

   Promoter

   GlnS (constitutive)

   Available Since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. DCX-RFP

   Plasmid

   #32851

   Depositor

   Joseph Gleeson

   Article

   Tanaka et al J Cell Biol. 2004 Jun 7. 165(5):709-21.

   Insert

   DCX (DCX Human)
   

   Tags

   DsRed2

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   Nov. 15, 2011

   Availability

   Academic Institutions and Nonprofits only

4. pHH0103_TP53BP2-1/1

   Plasmid

   #91503

   Purpose

   Protein expression and purification of human SH3 domain construct TP53BP2-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   TP53BP2-1/1 (TP53BP2 Human)
   

   Expression

   Bacterial

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFTK094-LM-AMA002.0

   Plasmid

   #171366

   Purpose

   Entry Vector compatible, fungal replicating, shuttle backbone vector from the Fungal Modular Cloning ToolKit.

   Depositor

   Arnold Driessen

   Article

   Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Mutation

   n/a

   Available Since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRSET-WHaloCaMP1a

   Plasmid

   #205304

   Purpose

   Expression of WHaloCaMP1a in E. coli

   Depositor

   Eric Schreiter

   Article

   Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

   Insert

   WHaloCaMP1a
   

   Expression

   Bacterial

   Available Since

   Aug. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDEST17-hOPTN

   Plasmid

   #23053

   Depositor

   Jon Ashwell

   Article

   Zhu et al Curr Biol. 2007 Aug 21. 17(16):1438-43.

   Insert

   optineurin (OPTN Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   N/A

   Available Since

   May 3, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPTDH

   Plasmid

   #66183

   Purpose

   Expresses phosphite dehydrogenase in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Watanabe et al ChemCatChem 2013 Dec;5(12):3835-3840

   Insert

   Phosphite Dehydrogenase
   

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   E175A, A176R

   Promoter

   T7 promoter

   Available Since

   June 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRARE-MBP-DEST

   Plasmid

   #84650

   Purpose

   Gateway cloning vector expresses MBP-tagged proteins under the control of the IPTG– inducible ptac promoter and also expresses several tRNAs that are rare in E. coli.

   Depositor

   Neil Olszewski

   Article

   Steiner et al Plant Cell. 2012 Jan;24(1):96-108. doi:

   Type

   Empty backbone

   Tags

   MBP

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Sept. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSF-CjPglB-LpxE

    Plasmid

    #128389

    Purpose

    Expression of C. jejuni PglB and F. tularensis LpxE in E. coli

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Inserts

    PglB oligosaccharylktransferase
    LpxE phosphatase
    

    Tags

    Flag tag

    Expression

    Bacterial

    Promoter

    Ptac and pBAD

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX4T-1 human RNF146 WWE (99-183)

    Plasmid

    #132613

    Purpose

    expresses human RNF146 WWE (99-183) in E. coli

    Depositor

    Wenqing Xu

    Article

    Wang et al Genes Dev. 2012 Feb 1;26(3):235-40. doi:

    Insert

    RNF146 WWE (99-183)
    

    Expression

    Bacterial

    Available Since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOGG012

    Plasmid

    #113986

    Purpose

    Vector construction module. Partition genes (parABCDE) for plasmid stability in absence of antibiotic selection for construction of Golden Gate cloning vectors

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    Partition genes (parABCDE)
    

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Available Since

    Jan. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBFC1207

    Plasmid

    #186694

    Purpose

    VcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, GmR+sfGFP cargo, with ET-Seq (AsiSI+SbfI) restriction sites flanking Tn7 Right End

    Depositor

    Jennifer Doudna

    Article

    Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:

    Inserts

    tnsA
    tnsB
    tnsC
    tnsD
    tniQ
    cas8-cas5 fusion
    cas7
    cas6
    Vc_2xBsaI_NT (Guide Stuffer) crRNA
    Tn7 transposon
    GmR+sfGFP VcDART Cargo
    SbfI+AsiSI dual restriction site for donor plasmid removal during ET-Seq
    

    Expression

    Bacterial

    Promoter

    PLac

    Available Since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTorPE-R-GECO1

    Plasmid

    #32465

    Depositor

    Robert Campbell

    Article

    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

    Insert

    R-GECO1.0
    

    Use

    Lab constructed

    Tags

    6x His and Modified TorA MNNNDLFQASRRRFLAQLG[G to…

    Expression

    Bacterial

    Mutation

    Substitutions relative to the mApple-derived anal…

    Available Since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a-Rx3(A7)

    Plasmid

    #35525

    Depositor

    Robert Cohen

    Article

    Sims et al Nat Methods. 2012 Feb 5. doi: 10.1038/nm

    Insert

    His6-Rx3(A7)
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Changed G2 to P

    Promoter

    T7

    Available Since

    April 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. hDHFR

    Plasmid

    #184707

    Purpose

    bacterial expression of human DHFR

    Depositor

    Peter Wright

    Article

    Bhabha et al FEBS Lett. 2011 Nov 16;585(22):3528-32.

    Insert

    DHFR (DHFR Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAX1_HS_gpN:SnoopTag

    Plasmid

    #225195

    Purpose

    Used for adding C-terminal SnoopTag to the E. coli HS P2 phage major capsid protein

    Depositor

    Travis Wiles

    Article

    Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

    Insert

    gpN homology arms and SnoopTag
    

    Expression

    Bacterial

    Available Since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. Enterovirus A71 (EV-A71) isolate TW-2553-1998 3C protease

    Plasmid

    #228636

    Purpose

    Bacterial expression of the A71 3C protease (isoform B) used in crystallisation

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    Enterovirus A71 3C protease isoform B
    

    Tags

    His-SUMO

    Expression

    Bacterial

    Available Since

    Feb. 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pUC19-HDRT-CD3z-truncCARgsg(anti-CD19)

    Plasmid

    #215759

    Purpose

    HDR template to insert a truncated CD3-zeta-deficient CD19-CAR into CD247 exon 2 (enhanced expression by GSG-2A linker)

    Depositor

    Dimitrios Wagner

    Article

    Kath et al Blood. 2024 Mar 17:blood.2023020973. doi

    Insert

    CD247-truncCD19CAR-GSG (CD19, CD247 Human)
    

    Tags

    IgG1 CH3

    Expression

    Bacterial

    Available Since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. EV-A71 2A protease; aliases: Enterovirus EV71 2A protease, 6xHis-SUMO-TEV tag, self cleaving

    Plasmid

    #204810

    Purpose

    Bacterial expresison of Enterovirus A71 2A protease

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    A71 2A protease
    

    Expression

    Bacterial

    Available Since

    Dec. 18, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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