34,231 results

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pNC02

Plasmid

#233644

Purpose

For bacterial expression of sfGFP c-terminally tagged with VitelloTagB.

Depositor

Zak Swartz

Publication

Clarke et al Development. 2024 Oct 15;151(20):dev2028

Insert

sfGFP with c-terminal VitelloTagB

Tags

6-His

Expression

Bacterial

Available since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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pBFC0993

Plasmid

#231993

Purpose

CRISPRi-ART plasmid encoding aTc-inducible dRfxCas13d and constitutive crRNA with negative control (RFP-targeting) spacer

Depositor

Brady Cress

Publication

Adler et al Nat Microbiol. 2025 Mar;10(3):694-709. d

Inserts

dRfxCas13d
crRNA with negative control (RFP-targeting) spacer

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

J23119 and pTet

Available since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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PS6K-mcherry-iATPsnFR1.1

Plasmid

#227238

Purpose

Inducible ATP sensor for E. coli

Depositor

Fuzhong Zhang

Publication

Mu et al Nat Commun. 2024 Jun 21;15(1):5299. doi:

Insert

iATPsnFR1.1

Tags

mCherry

Expression

Bacterial

Available since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pET29b_1_anti-GFP-nanobody

Plasmid

#210394

Purpose

Express anti-GFP nanobody in E. coli

Depositor

Magnus Rueping

Publication

Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

Insert

1_anti-GFP-nanobody

Tags

6xHis

Expression

Bacterial

Available since

Jan. 13, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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SUMO-PNGaseF

Plasmid

#228498

Purpose

10xHis-SUMO (Smt3) tagged PNGaseF (E. coli based protein expression)

Depositor

Richard Baker

Insert

ngl

Tags

10xHis-SUMO(Smt3)

Expression

Bacterial

Promoter

T7 Promoter

Available since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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Xla.Tubb2:hGCGR-TEVcs-QF;he1.1:CFP

Plasmid

#218857

Purpose

Xla.Tubb2 promoter driving expression of human glucagon receptor, TEV protease recognition site, QF fusion protein for use in trans-Tango.

Depositor

Marnie Halpern

Publication

Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

Insert

hGCGR (GCGR Human)

Tags

QF fusion protein

Expression

Bacterial

Available since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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Xla.Tubb2:hArr-TEV;he1.1:eYFP

Plasmid

#218859

Purpose

Xla.Tubb2 promoter driving expression of human Arrestin-TEV protease fusion protein for use in trans-Tango.

Depositor

Marnie Halpern

Publication

Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

Insert

hArr

Tags

human Arrestin-Tobbaco Etch Virus protease fusion…

Expression

Bacterial

Available since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pQFmcs-2H12.D11

Plasmid

#221183

Purpose

cdGreen2 expressed from a cumate-inducible promoter (Internal Lab ID: UJ11216)

Depositor

Urs Jenal

Publication

Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

Insert

cdGreen2

Expression

Bacterial

Promoter

PQ5 (cumate-inducible)

Available since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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TadCBE-Cas9NG

Plasmid

#225093

Purpose

Plasmid for bacterial purification of codon-optimized TadCBE-Cas9NG

Depositor

Vijay Sankaran

Publication

Martin-Rufino et al Science. 2025 Apr 4;388(6742):52-59. doi

Insert

TadCBE-Cas9NG

Use

CRISPR

Tags

8xHis

Expression

Bacterial

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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PT7lacO-EF-Tu-His8

Plasmid

#224370

Purpose

Express EF-Tu protein for his-tagged purification

Depositor

Richard Murray

Publication

Zhang et al ACS Synth Biol. 2025 May 16;14(5):1496-1

Insert

EF-Tu (tufB E. coli)

Tags

His8

Expression

Bacterial

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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PT7lacO-RF1-His6

Plasmid

#224372

Purpose

Express RF1 protein for his-tagged purification

Depositor

Richard Murray

Publication

Zhang et al ACS Synth Biol. 2025 May 16;14(5):1496-1

Insert

RF1 (prfA E. coli)

Tags

His6

Expression

Bacterial

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pYAL_HLA-A2_MART1

Plasmid

#171177

Purpose

Displays MART-1 peptide in HLA-A*02 as a single-chain trimer

Depositor

Michael Birnbaum

Publication

Huisman et al Methods Mol Biol. 2022;2491:263-291. doi

Insert

MART1_HLA-A2*01 single-chain trimer fusion

Expression

Bacterial and Yeast

Promoter

GAL1

Available since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMP11

Plasmid

#215559

Purpose

pKD46 with constitutively expressed Cas9 and an aTc gRNA targeting the ColE1 origin

Depositor

Brian Pfleger

Publication

Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

Inserts

Cas9
gRNA targeting pBR322 origin of replication

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

J23105 and tet

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGP-RSET-iGABASnFR2-6xHis

Plasmid

#218885

Purpose

Bacterial expression of improved GABA sensor (positive change in fluorescence)

Depositor

GENIE Project

Publication

Kolb et al eLife. 2026 Mar 18;14:RP108319. doi: 10.

Insert

iGABASnFR2

Tags

6xHis

Expression

Bacterial

Mutation

S99A F102Y F104Y L178S

Promoter

T7

Available since

May 14, 2024

Availability

Academic Institutions and Nonprofits only

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pAMS823 His6-MBP-3C-ORF2p (238-1061, ORFeus-Hs) in pET41

Plasmid

#213024

Purpose

Expresses human LINE-1 ORF2p Core (238-1061) in E. coli as an N-MBP fusion

Depositor

Martin S. Taylor, Oliver Weichenrieder

Publication

Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

Insert

LINE-1 ORF2p (238-1061)

Tags

His6-MBP-3C

Expression

Bacterial

Promoter

T7 / Lac

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pMApra-Ptac

Plasmid

#218357

Purpose

Empty vector with lacIq-Ptac expression system

Depositor

Michael Gebhardt

Publication

Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

Type

Empty backbone

Expression

Bacterial

Available since

May 2, 2024

Availability

Academic Institutions and Nonprofits only

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mTB3-BC2 nanobody

Plasmid

#210208

Purpose

Type 3 phage-display vector that contains kanamycin resistance and displays the BC2 nanobody at the N-terminus of mature protein III.

Depositor

Brian Kay

Publication

Grahn et al Methods Mol Biol. 2023;2702:191-203. doi

Insert

BC2 Nanobody

Expression

Bacterial

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pETM43 p62 FL (1-440)

Plasmid

#210425

Purpose

Bacterial expression of MBP-p62 FL (1-440) with 3C cleavage site

Depositor

Carsten Sachse

Publication

Tarafder et al Methods Mol Biol. 2019;1880:3-15. doi: 1

Insert

p62 FL (NUP62 Human)

Tags

6xHis and MBP

Expression

Bacterial

Available since

Feb. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCR8-attL1-pegRNA-attR5

Plasmid

#213053

Purpose

Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attR5

Depositor

Bing Yang

Publication

Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

Insert

sgRNA

Expression

Bacterial

Promoter

35S-CmYCLV-AtU6

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pNBU2_repA3

Plasmid

#211912

Purpose

Replicative vector for protein expression in Bacteroidales with an aTc inducible promoter, terminator, and a barcoding site.

Depositor

Adam Arkin

Publication

Huang et al Nat Commun. 2024 Aug 5;15(1):6618. doi:

Type

Empty backbone

Expression

Bacterial

Available since

Feb. 2, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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