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PB6K-mcherry-iATPsnFR1.1

Plasmid

#227239

Purpose

Inducible ATP sensor for Pseudomonas putida

Depositor

Fuzhong Zhang

Article

Mu et al Nat Commun. 2024 Jun 21;15(1):5299. doi:

Insert

iATPsnFR1.1

Expression

Bacterial

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pZa pBAD csgA CBDcex pLac Ara csgGEF

Plasmid

#226378

Purpose

Plasmid carries csgA and csgG,E,F genes of curli operon

Depositor

Urartu Seker

Article

Koksaldi et al Biosens Bioelectron. 2024 Aug 10;264:116

Insert

csgA and csgG,E,F genes of curli operon

Expression

Bacterial

Promoter

pBAD

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pBbS8c-ddcpf1-Δ

Plasmid

#153039

Purpose

For CRISPRi. Arabinose-inducible ddcpf1 gene with CRISPR array lacking a gene-specific spacer.

Depositor

Nigel Scrutton

Article

Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

Insert

Ascpf1

Expression

Bacterial

Mutation

E993A

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pTW084b

Plasmid

#116894

Purpose

Activity-independent SE-PACE AP

Depositor

David Liu

Article

Wang et al Nat Chem Biol. 2018 Aug 20. pii: 10.1038

Inserts

g3N
434-cI/scFv m3

Expression

Bacterial

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pCK351

Plasmid

#129696

Purpose

For insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + terminator ECK120034435

Depositor

John Heap

Article

Kelly et al Microorganisms. 2019 Aug 16;7(8). pii: m

Inserts

PrhaBAD
yfp
rhaS
Terminator ECK120034435

Expression

Bacterial

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pIB165

Plasmid

#90188

Purpose

E. coli - Streptococci shuttle plasmid for gene expression in streptococci with P23 promoter

Depositor

Indranil Biswas

Article

Biswas et al Microbiology. 2008 Aug;154(Pt 8):2275-82

Type

Empty backbone

Tags

His-tag and Strep-tag

Expression

Bacterial

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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ELAC1

Plasmid

#39097

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

ELAC1 (ELAC1 Human)

Tags

Tev His Flag

Expression

Bacterial

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pNIC28-BSA4-G-L11

Plasmid

#127830

Purpose

Production of recombinant human ribosomal protein L11

Depositor

Ivanhoe Leung

Article

Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

Insert

RPL11 (RPL11 Human)

Expression

Bacterial

Promoter

T7

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pTSA-13

Plasmid

#17327

Depositor

Takeshi Sano

Article

Sano et al J Biol Chem. 1995 Nov 24. 270(47):28204-

Insert

streptavidin

Expression

Bacterial

Mutation

Truncated to include only amino acids 16-133 of m…

Available Since

Feb. 25, 2008

Availability

Academic Institutions and Nonprofits only

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WasCFP /pQE-30

Plasmid

#79858

Purpose

Cyan-green fluorescent protein with tryptophan-based chromophore in equilibrium between anionic and neutral states.

Depositor

Konstantin Lukyanov

Article

Sarkisyan et al Sci Rep. 2012;2:608. doi: 10.1038/srep00

Insert

WasCFP

Tags

HisTag

Expression

Bacterial

Mutation

Cyan fluorescent protein mCerulean with substitut…

Promoter

T5 promoter / lac operator

Available Since

Sept. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pScI_dCas9-CDA

Plasmid

#108549

Purpose

Bacterial Target-AID vector

Depositor

Akihiko Kondo

Article

Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

Insert

dCas9-PmCDA1

Tags

Flag

Expression

Bacterial

Mutation

D10A and H840A for SpCas9

Promoter

lambda OR

Available Since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX6p-1-BCCP-PupE

Plasmid

#113403

Purpose

Expresses BCCP-PupE in bacteria

Depositor

Min Zhuang

Article

Liu et al Nat Methods. 2018 Aug 13. pii: 10.1038/s

Insert

BCCP-PupE

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-PFO

Plasmid

#92109

Purpose

Recombinant expression in E.Coli

Depositor

Feng Shao

Article

Ding et al Nature. 2016 Jul 7;535(7610):111-6. Epub

Insert

PFO

Tags

6xHis

Expression

Bacterial

Mutation

Delta27aa

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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C1-82 pComb3xss anti-cFos nanobody

Plasmid

#145829

Purpose

Bacterial expression of anti-cFos (Human) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-cFos (Human) recombinant llama nanobody C1-82 (FOS L. glama (llama))

Tags

6xHis-HA

Expression

Bacterial

Promoter

lac

Available Since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

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pYTRW26K_1Ti1

Plasmid

#177292

Purpose

genomic integration of genes (inserted in I-SceI-site) via rrn-interposon/SacB with TcR and eYFP

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

PsacB-sacB

Use

Synthetic Biology; Yeast expression, rrn genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pDWJ26

Plasmid

#207603

Purpose

GFP-AIA expression

Depositor

Colin Kleanthous

Article

Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

GFP-AIA

Expression

Bacterial

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-HsSENP3

Plasmid

#71467

Purpose

Encodes human full length SENP3. Suitable for in vitro transcription/translation (T7).

Depositor

Jorge Eduardo Azevedo

Article

Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

Insert

SUMO1/sentrin specific peptidase 3 (SENP3 Human)

Tags

Hexahistidine

Expression

Bacterial

Mutation

Gene optimised for E. coli expression (Genscript)

Promoter

T7

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pcI-ts,ind+-(NSP8)

Plasmid

#160656

Purpose

Expresses codon optimized SARS-CoV-2 NSP8 in E. coli

Depositor

Kenneth Johnson

Article

Dangerfield et al iScience. 2020 Nov 28:101849. doi: 10.10

Insert

SARS-CoV-2 NSP8 (ORF1ab Synthetic)

Expression

Bacterial

Available Since

Dec. 10, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pRARE-MBP-DEST

Plasmid

#84650

Purpose

Gateway cloning vector expresses MBP-tagged proteins under the control of the IPTG– inducible ptac promoter and also expresses several tRNAs that are rare in E. coli.

Depositor

Neil Olszewski

Article

Steiner et al Plant Cell. 2012 Jan;24(1):96-108. doi:

Type

Empty backbone

Tags

MBP

Expression

Bacterial

Promoter

tac

Available Since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pDDYFP-A

Plasmid

#40288

Depositor

Robert Campbell

Article

Alford et al ACS Synth Biol. 2012 Dec 21;1(12):569-75

Insert

ddYFP-A

Tags

His

Expression

Bacterial

Available Since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only