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pCORE-Hp53

Plasmid

#72242

Purpose

Contains the CORE cassette with hyg and human p53 gene markers

Depositor

Michael Resnick, Francesca Storici

Article

Storici et al Genet Eng (N Y). 2003;25:189-207.

Inserts

p53 (TP53 Human)
hyg

Expression

Bacterial

Mutation

V122A mutation

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pET23a-HsRPS27a

Plasmid

#69561

Purpose

Bacterial expression; in vitro transcription/translation (T7)

Depositor

Jorge Eduardo Azevedo

Article

Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

Insert

RPS27A (RPS27A Human)

Expression

Bacterial

Promoter

T7

Available Since

Dec. 11, 2015

Availability

Academic Institutions and Nonprofits only
pET28a-HsSENP3

Plasmid

#71467

Purpose

Encodes human full length SENP3. Suitable for in vitro transcription/translation (T7).

Depositor

Jorge Eduardo Azevedo

Article

Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

Insert

SUMO1/sentrin specific peptidase 3 (SENP3 Human)

Tags

Hexahistidine

Expression

Bacterial

Mutation

Gene optimised for E. coli expression (Genscript)

Promoter

T7

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pET23a-Mm/HsUBA52

Plasmid

#69560

Purpose

Bacterial expression; in vitro transcription/translation (T7)

Depositor

Jorge Eduardo Azevedo

Article

Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

Insert

UBA52 (UBA52 Human)

Expression

Bacterial

Promoter

T7

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only
AP575-1

Plasmid

#71277

Purpose

Expresses 3Xflag::tagRFP::myc in bacteria

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2015 Sep;201(1):47-54. doi: 10

Insert

tagRFP

Tags

3xFlag and myc

Expression

Bacterial

Available Since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pJDC182

Plasmid

#60854

Purpose

Expresses firefly luciferase

Depositor

Jeffrey Cirillo

Article

Chang et al PLoS One. 2014 Sep 29;9(9):e108341. doi:

Insert

Firefly luciferase (optimized for Mycobacteria)

Expression

Bacterial

Mutation

codon optimized for Mycobacteria

Promoter

hsp60

Available Since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-Zeo-ecfp

Plasmid

#65038

Purpose

ZeoR mini-Tn7 vector, mobilizable, for CFP tagging bacteria

Depositor

Herbert Schweizer

Article

Choi et al Nat Protoc. 2006;1(1):153-61.

Insert

ecfp

Expression

Bacterial

Available Since

Nov. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pCR8/GW/TOPO BMI

Plasmid

#69796

Purpose

Gateway entry vector for BMI

Depositor

Gerard Cagney

Insert

BMI (BMI1 Human)

Expression

Bacterial

Available Since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only
AP682-1

Plasmid

#70050

Purpose

Expresses TEV::eGFP::myc::3Xflag in bacteria

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2015 Sep;201(1):47-54. doi: 10

Insert

eGFP

Tags

TEV and myc::3Xflag

Expression

Bacterial

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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CYP2C19

Plasmid

#69606

Purpose

For protein expression of CYP2C19

Depositor

Joyce Goldstein

Insert

CYP2C19 (CYP2C19 Human)

Expression

Bacterial

Mutation

deletion of AA1-8 and mutation I331V

Available Since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pCLC-mEos2

Plasmid

#38010

Depositor

Xiaowei Zhuang

Article

Jones et al Nat Methods. 2011 Jun;8(6):499-508. Epub

Insert

Clathrin, light polypeptide (LCa) (Clta Mouse)

Tags

mEos2

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Sept. 29, 2015

Availability

Academic Institutions and Nonprofits only

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MP3

Plasmid

#69650

Purpose

Untargetted Bacterial Mutagenesis

Depositor

David Liu

Article

Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

Insert

dnaQ926 dam

Expression

Bacterial

Promoter

E. coli PBAD

Available Since

Sept. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pBSMrnaStrp

Plasmid

#67508

Purpose

Express the tRNAMet scaffold with the streptavidin aptamer tag

Depositor

Luc Ponchon

Article

Ponchon et al Nat Protoc. 2009;4(6):947-59. doi: 10.10

Insert

modified tRNAMet with streptavidin aptamer

Expression

Bacterial

Promoter

lpp

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
rnALDH

Plasmid

#67920

Purpose

Expresses aldehyde dehydrogenase from Rattus norvegicus, codon optimized for expression in E. coli

Depositor

Justin Siegel

Insert

Rattus norvegicus aldehyde dehydrogenase (Aldh3a1 Rat)

Tags

6x-His

Expression

Bacterial

Promoter

T7

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only
pHis-zfEpoa

Plasmid

#65611

Purpose

Express soluble His-tagged zebrafish erythropoietin in E. coli

Depositor

Petr Bartunek

Article

Stachura et al Blood. 2009 Jul 9;114(2):279-89. doi: 10

Insert

erythropoietin (epoa Zebrafish)

Tags

(His)6

Expression

Bacterial

Promoter

T7

Available Since

Sept. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pSMART-Mev1

Plasmid

#65815

Purpose

NADPH Dependent Mevalonic Acid Production Construct, Low Phosphate Dependent E. coli Expression

Depositor

Michael Lynch

Inserts

mvaE: bifunctional acetoacetyl-CoA thiolase, and NADPH dependent HMG-CoA reductase
mvaS: hydroxymethylglutaryl-CoA synthase

Expression

Bacterial

Promoter

insulated mipAp from E. coli and insulated waaHp…

Available Since

Sept. 4, 2015

Availability

Academic Institutions and Nonprofits only

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PT7-deGFP-MGapt

Plasmid

#67741

Purpose

T7 promoter expressing deGFP-MG aptamer fusion

Depositor

Richard Murray

Article

Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

Insert

UTR1-deGFP-MGapt-T7term

Use

Synthetic Biology

Expression

Bacterial

Promoter

PT7

Available Since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pRF-QacR

Plasmid

#49369

Depositor

Christopher Voigt

Article

Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

Insert

QacR repressor

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pBW115lac-hrpR

Plasmid

#61434

Purpose

pAPT110 encoding rbsH-hrpR-ter

Depositor

Martin Buck, Baojun Wang

Article

Wang et al Nat Commun. 2011 Oct 18;2:508. doi: 10.1

Insert

rbsH-hrpR-ter

Expression

Bacterial

Promoter

pLac

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pC239

Plasmid

#65953

Purpose

N2 inverter plasmid for two-strain system

Depositor

Matthew Bennett

Article

Chen et al Science. 2015 Aug 28;349(6251):986-9. do

Inserts

AiiA
LacI

Tags

LAA tagged

Expression

Bacterial

Promoter

pCin and pCin*

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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