30,262 results

1. Ubiquitin K48C

   Plasmid

   #86588

   Purpose

   Bacterial expression of Ubiquitin K48C for cross linking studies

   Depositor

   Sharon Rozovsky

   Article

   Liu et al J Am Chem Soc. 2017 Feb 10. doi: 10.1021

   Insert

   Ubiquitin (UBC Human)
   

   Use

   Tags

   His6-MBP

   Expression

   Bacterial

   Mutation

   TEV site; K48C

   Promoter

   Tac

   Available since

   Feb. 24, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pGBMCS-SortA

   Plasmid

   #21931

   Depositor

   Fuyuhiko Inagaki

   Article

   Kobashigawa et al J Biomol NMR. 2009 Mar . 43(3):145-50.

   Insert

   sortase (srtA Staphylococcus aureus (strain N315))
   

   Use

   Tags

   GB1 and His-tag

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-59

   Promoter

   Available since

   Oct. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

3. pET29_LuxSit_i

   Plasmid

   #194299

   Purpose

   Bacterial expression of the de novo luciferase, LuxSit-i

   Depositor

   David Baker, Andy Yeh

   Article

   Yeh et al Nature. 2023 Feb;614(7949):774-780. doi:

   Insert

   LuxSit-i
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX6p1-Rbck1

   Plasmid

   #63135

   Purpose

   Expression of human RBCK1/HOIL-1L

   Depositor

   Titia Sixma

   Article

   Smit et al EMBO J. 2012 Oct 3;31(19):3833-44. doi:

   Insert

   HOIL-1L (RBCK1 Synthetic, Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Synthetic based on human protein sequence

   Promoter

   tac

   Available since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

5. bdSUMO-E5TAT-GFP-HiBit

   Plasmid

   #170993

   Purpose

   For bacterial expression of bdSUMO tagged muGFP with E5TAT peptide and NanoLuc complementation peptide variant #86

   Depositor

   Angus Johnston

   Article

   Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

   Insert

   14x-His-bdSUMO-E5TAT-muGFP-HiBiT
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. 6xHis(TEV)-Drp1-StrepII

   Plasmid

   #174422

   Purpose

   Expresses human Drp1 Isoform 3 in bacteria

   Depositor

   Thomas Pucadyil

   Article

   Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

   Insert

   Drp1
   

   Use

   Tags

   6xHis(TEV) and StrepII

   Expression

   Bacterial

   Mutation

   None

   Promoter

   T7

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pQE-60NA-proinsulin-mEGFP

   Plasmid

   #160464

   Purpose

   Encodes a fusion of human proinsulin and the monomeric enhanced EGFP. This construct can be used as a negative control for superfolder activity in bacteria

   Depositor

   Benjamin Glick

   Article

   Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

   Insert

   proinsulin-mEGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. NCD-GFP

   Plasmid

   #24507

   Depositor

   Ron Vale

   Article

   Case et al Cell. 1997 Sep 5. 90(5):959-66.

   Insert

   NCD (ncd Fly)
   

   Use

   Tags

   GFP and His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 30, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pET mCerulean LIC cloning vector (H6-mCerulean)

   Plasmid

   #29726

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   6xHis, AviTag (biotin), TEV cleavage site, and mC…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 15, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGI3EM22C

    Plasmid

    #135488

    Purpose

    Agrobacterium T-DNA with divergent H2A/Bpr driving LifeAct-tdTomato and hph

    Depositor

    Nicolas Buchler

    Article

    Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

    Insert

    hph SpunH2A/Bpr LifeAct-tdTomato
    

    Use

    Tags

    LifeAct

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    SpunH2A/Bpr (divergent)

    Available since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCYC2

    Plasmid

    #43970

    Depositor

    Hal Alper

    Article

    Crook et al Nucleic Acids Res. 2011 Aug;39(14):e92.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    CYC1

    Available since

    April 9, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pGEX-2T_PV1_VP1

    Plasmid

    #202071

    Purpose

    Bacterial expression of VP1 protein from poliovirus 1 (PV1). Contains N-terminal GST-tag and C-terminal His6-tag.

    Depositor

    Vesa Hytönen

    Article

    Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e053

    Insert

    PV1 VP1 protein with Histag
    

    Use

    Tags

    6xHis-tag and GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRSET-pr-mEos3.2 (mEosFP3.2-A69T)

    Plasmid

    #99224

    Purpose

    Bacterial expression of the primed conversion capable protein pr-mEos3.2 (mEos3.2-A69T).

    Depositor

    Periklis Pantazis

    Article

    Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

    Insert

    pr-mEos3.2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A69T for primed conversion applications

    Promoter

    T7

    Available since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. Parp14-c011

    Plasmid

    #173098

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    PARP14 (PARP14 Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    K791-F978

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. EV-A71 3C protease; aliases: Enterovirus A71 3C protease, non cleavable C-term 6xHis tag

    Plasmid

    #204816

    Purpose

    Bacterial expresison of Enterovirus A71 3C protease

    Depositor

    Frank von Delft, ASAP Discovery

    Insert

    EV A71 3C protease
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 17, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pEYN12-mflon

    Plasmid

    #165581

    Purpose

    Lon protease from Mesoplasma florum

    Depositor

    Daniel Ducat

    Article

    Sakkos et al ACS Synth Biol. 2021 Jul 16;10(7):1667-1

    Insert

    lon protease
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptrc

    Available since

    March 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. NylB'_pET21b

    Plasmid

    #215416

    Purpose

    Expression construct for NylB' gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    NylB'
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCHAP3_Sap

    Plasmid

    #206849

    Purpose

    A plasmid containing a 17,271 bp fragment of the P. tricornutum chloroplast genome. The yeast marker URA3 was domesticated for SapI and integrated into the chloroplast fragment.

    Depositor

    Bogumil Karas

    Article

    Walker et al Plant Physiol. 2023 Dec 19:kiad670. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Jan. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCHAP2

    Plasmid

    #206847

    Purpose

    A plasmid containing a 15,249 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.

    Depositor

    Bogumil Karas

    Article

    Walker et al Plant Physiol. 2023 Dec 19:kiad670. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Jan. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCHAP1

    Plasmid

    #206846

    Purpose

    A plasmid containing a 14,519 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.

    Depositor

    Bogumil Karas

    Article

    Walker et al Plant Physiol. 2023 Dec 19:kiad670. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Jan. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge