30,807 results

1. pEvol-pAcFRS.1.t1

   Plasmid

   #73545

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAG

   Depositor

   Farren Isaacs

   Article

   Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

   Inserts

   pAcFRS.1.t1
   pAcFRS.1.t1
   

   Expression

   Bacterial

   Available Since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. N-BLa 1.2

   Plasmid

   #88999

   Purpose

   BLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.2 fusionprotein containing the N-terminal fragment of the split β-lactamase.

   Depositor

   Dieter Langosch

   Article

   Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103

   Insert

   N-BLa 1.2 fusion protein
   

   Tags

   Flag

   Expression

   Bacterial

   Promoter

   araBAD

   Available Since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. C-BLa 1.2

   Plasmid

   #89000

   Purpose

   BLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.2 fusionprotein containing the C-terminal fragment of the split β-lactamase.

   Depositor

   Dieter Langosch

   Article

   Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103

   Insert

   C-BLa 1.2 fusion protein
   

   Tags

   Flag

   Expression

   Bacterial

   Promoter

   araBAD

   Available Since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAD-LyseR (in lacZ deficient strain)

   Plasmid

   #99245

   Purpose

   For autolysate production in lacZ deficient (lac operon deletion) E. coli BL21-Gold-dLac (DE3) strain: constitutively expresses phage lambda endolysin (gene R)

   Depositor

   Jeff Hasty

   Article

   Didovyk et al ACS Synth Biol. 2017 Aug 10. doi: 10.102

   Insert

   phage lambda gene R (R phage lambda)
   

   Expression

   Bacterial

   Promoter

   beta-lactamase promoter

   Available Since

   Oct. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. BARD1 26-327 pET28a

   Plasmid

   #12646

   Depositor

   Rachel Klevit

   Article

   Brzovic et al Mol Cell. 2006 Mar 17. 21(6):873-80.

   Insert

   BRCA1 binding partner for a Ring domain E3 Ub-ligase (BARD1 Human)
   

   Expression

   Bacterial

   Available Since

   July 31, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEM-Cas9HF1-indRecA56

   Plasmid

   #102294

   Purpose

   Modified from pEM-Cas9HF1 (Addgene ID: 89961) to co-express inducible recA56 to block recA-mediated double-strand break repair.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Inserts

   Cas9HF1
   recA56
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   N497A/R661A/Q695A/Q926A

   Promoter

   pTet

   Available Since

   April 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAN1717

   Plasmid

   #74696

   Purpose

   RPU standard plasmid

   Depositor

   Christopher Voigt

   Article

   Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

   Insert

   Insulated J23101-YFP
   

   Expression

   Bacterial

   Available Since

   May 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX-hXRCC1

   Plasmid

   #52274

   Purpose

   Bacterial expression of human XRCC1 with N-terminal GST-tag

   Depositor

   Primo Schaer

   Article

   Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

   Insert

   XRCC1 (XRCC1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   July 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTarget12f1

   Plasmid

   #171183

   Purpose

   Constitutive expression of sgRNA without donor DNA

   Depositor

   Kohsuke Honda

   Article

   Okano et al J Biosci Bioeng. 2021 Aug;132(2):120-124

   Insert

   sgRNA scaffold in the CRISPR/Cas12f1 system
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   J23119(SpeI) promoter

   Available Since

   June 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTriEx-RhoA FLARE.sc Biosensor T19N

    Plasmid

    #12152

    Depositor

    Klaus Hahn

    Article

    Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

    Insert

    RhoA Biosensor T19N (RHOA Human)
    

    Tags

    6xHis

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    RhoA T19N (dominant-negative mutation)*

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX-parkin

    Plasmid

    #227367

    Purpose

    For bacterial expression of parkin

    Depositor

    Kalle Gehring

    Article

    Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

    Insert

    Parkin (PRKN Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Codon usage optimized for E.coli expression.

    Available Since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pZa-tetO-csgB-CmR

    Plasmid

    #89580

    Purpose

    Express csgB curli protein

    Depositor

    Urartu Seker

    Article

    Abdelwahab et al Langmuir. 2017 Apr 7. doi: 10.1021/acs.l

    Insert

    csgB
    

    Expression

    Bacterial

    Available Since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX6P1-3xMBT_D355N

    Plasmid

    #46988

    Purpose

    Inducible expression of 3xMBT (D355N) from L3MBTL1 as a GST fusion protein in E.coli

    Depositor

    Or Gozani

    Article

    Moore et al Mol Cell. 2013 May 9;50(3):444-56. doi:

    Insert

    Triple MBT domain from human L3MBTL1 (L3MBTL1 Human)
    

    Tags

    GST and PreScission cleavage site

    Expression

    Bacterial

    Mutation

    Mutation aspartic acid 355 to asparagine (D355N);…

    Promoter

    tac

    Available Since

    Sept. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. AF1521-c002

    Plasmid

    #173109

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    AF_1521 (AF_RS07675 Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126))
    

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    KH-M1-L192

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJBL711

    Plasmid

    #140381

    Purpose

    Expresses OtrR protein under the control of T7-lacO promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-lacO-OtrR-T
    

    Tags

    TEV-6XHis

    Expression

    Bacterial

    Available Since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC18T-mini-Tn7T-Zeo-dsRedExpress

    Plasmid

    #65040

    Purpose

    ZeoR mini-Tn7 vector, mobilizable, for RFP tagging bacteria

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Protoc. 2006;1(1):153-61.

    Insert

    dsRedExpress
    

    Expression

    Bacterial

    Available Since

    Nov. 2, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. AF1521-c003

    Plasmid

    #173111

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    AF_1521 (AF_RS07675 Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126))
    

    Tags

    Nanoluc

    Expression

    Bacterial

    Mutation

    KH-M1-L192

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. His6_MBP_FXR2P_P474S_P493N

    Plasmid

    #198695

    Purpose

    Expression of human FXR2 protein in E. coli

    Depositor

    Simpson Joseph

    Article

    Edwards et al Sci Rep. 2020 Sep 28;10(1):15858. doi: 1

    Insert

    FXR2P (FXR2 Human)
    

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Changed proline 474 to serine and proline 493 asp…

    Promoter

    T7

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pZE-PalkB-GFP

    Plasmid

    #44381

    Depositor

    Hal Alper

    Article

    Reed et al J Biotechnol. 2012 Apr 15;158(3):75-9. d

    Inserts

    GFP
    PalkB
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PalkB

    Available Since

    April 17, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. MT1G

    Plasmid

    #105704

    Purpose

    Bacterial expression plasmids for production of recombinant metallothionein 1g

    Depositor

    Artur Krężel

    Insert

    Metallothionein 1g (MT1G Human)
    

    Tags

    Chiting Binding Domanin-Intein

    Expression

    Bacterial

    Available Since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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