32,926 results
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Plasmid#109782PurposeProtein expression and purification of GNA15_G-alphaDepositorInsertGNA15_G-alpha (GNA15 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S_Psyn-sgRNA500
Plasmid#149640Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloningDepositorTypeEmpty backboneUseCRISPRExpressionBacterialPromoterPflaB, PpQE30, PsynAvailable SinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC18/promSau/Lysostaphin
Plasmid#125766PurposeProduction of staphylolytic protein lysostaphin from a constitutive promoterDepositorInsertlysostaphin
ExpressionBacterialMutationsequence corresponding to the mature form proteinPromoterpemIK constitutive promoterAvailable SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
R726-M01-892: His6-Halotag-tev-Hs.RAF1(52-131)
Plasmid#159696PurposeE. coli protein expression of His6-Halotag-tev-Hs.RAF1(52-131)DepositorAvailable SinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
R989-X02-566: His6-MBP-tev-G-Hs.KRAS4b(1-169)
Plasmid#159539PurposeE. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169)DepositorAvailable SinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
R989-X08-566: His6-MBP-tev-G-Hs.KRAS4b(1-169) G12C
Plasmid#159540PurposeE. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169) G12CDepositorInsertHis6-MBP-tev-G-Hs.KRAS4b(1-169) G12C (KRAS Human)
TagsHis6-MBP-tevExpressionBacterialMutationG12CAvailable SinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pACDura3
Plasmid#138711PurposeExpresses group II intron Ll.LtrB and IEP (LtrA) from Lactoccus lactis under control of T7 promoter. Ll.LtrB is retargeted to insert into 635s locus of LacZ gene and ura3 gene is cloned inside.DepositorInsertura3 gene (URA3 Budding Yeast)
ExpressionBacterialMutationoptimized ura3 gene for expression in Escherichia…PromoterpEM7 promoterAvailable SinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSEVA421-Cas9tr
Plasmid#138709PurposePlasmid constitutively expressing the Cas9 nuclease and the non-coding tracrRNA from S. pyogenesDepositorInserttracrRNA-Cas9
UseCRISPR and Synthetic BiologyExpressionBacterialAvailable SinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSB3C5-phzSM
Plasmid#141105PurposeContains a phzS-phzM operon BioBrickDepositorInsertphzSM
ExpressionBacterialAvailable SinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
TwinStrep-SUMO-NLS-BhCas12b_v4
Plasmid#158615PurposeFor protein expression of BhCas12b_v4DepositorInsertNLS-BhCas12b_v4
Tags6xHIS-TwinStrep-SUMOExpressionBacterialAvailable SinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28b-GBP-MTn
Plasmid#154363PurposeBacterial expression of MTnDepositorInsertMTn
TagsHisExpressionBacterialAvailable SinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28-6His-SUMO-Nsp14
Plasmid#154956PurposeExpresses Nsp14 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coliDepositorInsert6His-SUMO-Nsp14 (ORF1ab SARS-CoV-2)
Tags6His-Thrombin-SUMOExpressionBacterialPromoterT7 PromoterAvailable SinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pETM33_Nsp10
Plasmid#156479PurposeBacterial expression of Sars-CoV2 Nsp10 protein with His-tag and GST-tagDepositorInsertNsp10 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
TagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQCascade-array1
Plasmid#140623PurposeExpresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-array1. The crRNA-array1 targets 8 different loci in Escherchia coli.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, crRNA-array1
UseCRISPR; TransposonExpressionBacterialAvailable SinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQCascade-IS1
Plasmid#140624PurposeExpresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-IS1. The crRNA-IS1 targets IS1 loci in Escherchia coli.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, crRNA-IS1
UseCRISPR; TransposonExpressionBacterialAvailable SinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJBL723
Plasmid#140393PurposeExpresses HucR protein under the control of T7-lacO promoterDepositorInsertT7-lacO-HucR-T
Tags6XHisExpressionBacterialAvailable SinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
WARS_pNIC-CTB10H
Plasmid#153058PurposeExpression construct for producing Avi tagged Tryptophanyl-tRNA synthetaseDepositorAvailable SinceJune 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
RARS_pNIC-Bio3
Plasmid#153055PurposeExpression construct for producing Avi tagged Arginyl-tRNA synthetaseDepositorAvailable SinceJune 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
AARS_C1_pNIC-Bio3
Plasmid#153046PurposeExpression construct for producing Avi tagged Alanyl-tRNA synthetaseDepositorAvailable SinceJune 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
TS_pLuxLac
Plasmid#140426PurposeToggle switch circuit responsive to AHL gradient.DepositorInsertsGFP
LacI
TetR
mCherry
UseSynthetic BiologyExpressionBacterialAvailable SinceJune 15, 2020AvailabilityAcademic Institutions and Nonprofits only