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34,232 results

Showing: 5621 - 5640 of 34232 results

  3. pdCRISPomyces-2BD

    Plasmid
    #234429
    Purpose
    Plasmid for Streptomyces containing gene of modified dCas9-BD protein and sequence of sgRNA cloning template
    Depositor
    Min-Kyu Oh
    Publication
    Kim et al Nat Commun. 2025 Jan 21;16(1):874. doi:
    Insert
    Modified dCas9(D10A, H840A) with polyaspartate linking
    Expression
    Bacterial
    Mutation
    Linked polyaspartate using glycine-serine linker
    Available since
    April 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    234429-plasmid-map-sequence-id-468849

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  4. p27-mVenus reporter (PB)

    Plasmid
    #190731
    Purpose
    The p27 reporter vector, which expresses mVenus-fused, mutant (K–) p27 protein with a defective CDK binding.
    Depositor
    Toshiro Sato
    Publication
    Ohta et al Nature. 2022 Jul 7. pii: 10.1038/s41586-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    mVenus
    p27
    Expression
    Bacterial and Mammalian
    Mutation
    p27-F62A-F64A
    Promoter
    EF1α
    Available since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    190731-plasmid-map-sequence-id-482793

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  5. pVP143

    Plasmid
    #222080
    Purpose
    Single component CRISPR-mediated base-editor for Agrobacterium genetic engineering visually marked with sfGFP for guide insertion and AeBlue for plasmid eviction.
    Depositor
    Wayne Parrott
    Publication
    Pennetti et al Biodes Res. 2025 Feb 27;7(1):100001. doi
    Insert
    dCas9-PmCDA1, sfGFP, sacB and AeBlue
    Use
    CRISPR
    Expression
    Bacterial
    Available since
    Jan. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    222080-plasmid-map-sequence-id-438352

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  6. pET28b-ULP1-405-621

    Plasmid
    #229224
    Purpose
    For expression of ULP1 protein
    Depositor
    Andy LiWang
    Insert
    ULP1 (ULP1 Budding Yeast)
    Expression
    Bacterial
    Mutation
    405-621 (truncated)
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    229224-plasmid-map-sequence-id-457073

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  16. pYHY3

    Plasmid
    #216109
    Purpose
    aTC-inducible expression of secretory BT3630 SP-NanoLuc via P1TDP-A21 promoter-RBS pair in Bacteroides species.
    Depositor
    Shannon Sirk
    Publication
    Yeh et al Nat Commun. 2024 Nov 11;15(1):9741. doi:
    Insert
    NanoLuc
    Use
    Synthetic Biology; E. coli-bacteroides shuttle ve…
    Tags
    3xFLAG tag and BT3630 signal peptide
    Expression
    Bacterial
    Promoter
    P1TDP-A21
    Available since
    May 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    216109-plasmid-map-sequence-id-434372

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  18. pRSF-G1(6/7FTrp)RS

    Plasmid
    #207621
    Purpose
    tRNA synthetase/tRNA pair for the in vivo incorporation of 6-Fluoro-L-Tryptophan (6FTrp) and 7-Fluoro-L-Tryptophan (7FTrp) into proteins in E. coli in response to the amber (TAG) codon
    Depositor
    Thomas Huber
    Publication
    Qianzhu et al J Am Chem Soc. 2024 Apr 30. doi: 10.1021
    Insert
    tRNA synthetase
    Expression
    Bacterial
    Promoter
    T7
    Available since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    207621-plasmid-map-sequence-id-410952

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Showing: 5621 - 5640 of 34232 results