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pUdO1a

Plasmid

#210226

Purpose

high-copy plasmid for bacterial expression

Depositor

François-Xavier Campbell-Valois

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pETM33_EIF4E_eiF4E

Plasmid

#178474

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

EIF4E_eiF4E (EIF4E Human, Synthetic)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pMRE152

Plasmid

#118502

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

sGFP2

Expression

Bacterial

Available Since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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human EB1 in PET28a for protein expression

Plasmid

#39297

Depositor

Tim Mitchison, Jennifer Tirnauer

Article

Tirnauer et al Mol Biol Cell. 2002 Oct . 13(10):3614-26

Insert

EB1 (MAPRE1 Human)

Tags

6HIS

Expression

Bacterial

Promoter

T7

Available Since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
pGP-FB-orig BA

Plasmid

#13420

Depositor

Keith Joung

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

orig BA

Tags

Gal11P

Expression

Bacterial

Available Since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

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pMPMA3dPlac-PpipB-GFPLVA

Plasmid

#23344

Depositor

Olivia Steele-Mortimer

Article

Ibarra et al Microbiology (Reading). 2010 Apr;156(Pt

Insert

GFP(LVA)

Tags

GFP(LVA)

Expression

Bacterial

Available Since

April 27, 2010

Availability

Academic Institutions and Nonprofits only

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AF1521-c003

Plasmid

#173111

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

AF_1521 (AF_RS07675 Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126))

Tags

Nanoluc

Expression

Bacterial

Mutation

KH-M1-L192

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pRAM18dSFA[MCS]

Plasmid

#84685

Purpose

Can be used for the transformation of rickettsia

Depositor

Ulrike Munderloh

Article

Burkhardt et al PLoS One. 2011;6(12):e29511. doi: 10.137

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pQmod2C-GG

Plasmid

#191347

Purpose

Clostridium expression vector (pBP1 origin, cmR) with drop-out Plac-RFP cassette flanked with BsaI sites

Depositor

Andrew Tolonen

Article

Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

Insert

Plac-RFP (IPTG-inducible red fluorescent protein)

Expression

Bacterial

Promoter

Plac

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pDSP31

Plasmid

#199373

Purpose

Encodes dual cistronic nuclear localized GCaMP6f and mScarlet-I optimized for C. elegans expression

Depositor

Hang Lu

Insert

NLS::GCaMP6f::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS

Expression

Bacterial

Mutation

no mutations, except added subcellular localizati…

Available Since

April 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pLG017 (AVAST type 1)

Plasmid

#157895

Purpose

Expresses the AVAST type 1 defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

AVAST type 1 (STAND ATPase with N-terminal serine protease)

Expression

Bacterial

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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HECTD1

Plasmid

#25183

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

hectd1.1273.1343 (HECTD1 Human)

Tags

His

Expression

Bacterial

Available Since

Aug. 9, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pET28a-PTBP1CCtoSS

Plasmid

#89157

Purpose

Recombinant expression of PTBP1 C250S,C251S in E.coli

Depositor

Frédéric Allain

Article

Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n

Insert

Polypyrimidine Tract Binding Protein 1 mutant Cys250Ser, Cys251Ser (PTBP1 Human)

Tags

hexa His

Expression

Bacterial

Mutation

mutations: Cys 250 to Ser, Cys 251 to Ser

Promoter

T7

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn5-155

Plasmid

#118537

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mScarlet-I

Use

Minitn5 delivery vector

Expression

Bacterial

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn5-135

Plasmid

#118521

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mScarlet-I

Use

Minitn5 delivery vector

Expression

Bacterial

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pSt-KO

Plasmid

#44566

Purpose

for generation of knockout of mycobacterial genes

Depositor

Vinay Nandicoori

Article

Parikh et al Appl Environ Microbiol. 2013 Mar;79(5):1

Type

Empty backbone

Use

E.coli-mycobacteria shuttle vector

Expression

Bacterial

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pST50Trc1-HISNDHFR

Plasmid

#63940

Purpose

Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

Depositor

Song Tan

Article

Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

Type

Empty backbone

Tags

6xHis; TEV cleavable

Expression

Bacterial

Promoter

T7

Available Since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pKAR2-Br512g3.2

Plasmid

#171201

Purpose

Expresses Br512g3.2 polymerase in E.coli

Depositor

Andrew Ellington

Article

Bhadra et al medRxiv 2022.08.09.22278605

Insert

Engineered Bst DNA Polymerase I Large Fragment

Tags

8x His tag; Villin Headpiece 47 amino acid (vHP47…

Expression

Bacterial

Promoter

T7

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pRECMYC-Ebu1

Plasmid

#240713

Purpose

pLAM12 vector backbone containing Ebu1 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155

Depositor

Seth Shipman

Article

González-Delgado et al bioRxiv 2025.06.16.660010

Insert

Ebu1 RT, Eco1 ncRNA, MspRecT

Expression

Bacterial

Mutation

WT

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pJAK184.tetR.PT5-3.dCas9

Plasmid

#232482

Purpose

optimized ahTet inducible dCas9 for insertion into C. difficile genome

Depositor

James Collins

Article

Chua et al Microbiol Resour Announc. 2025 Apr 28:e0

Insert

Catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system

Use

CRISPR

Expression

Bacterial

Promoter

TetR-PT5-3

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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