30,296 results

1. pDEST17-UbE2G2

   Plasmid

   #15791

   Depositor

   Wade Harper

   Article

   Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

   Insert

   Ubiquitin conjugating enzyme UbE2G2 (UBE2G2 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHis-hMTMR1

   Plasmid

   #100723

   Purpose

   Expression of human MTMR1 PH-PTP domain

   Depositor

   Byung Il Lee

   Article

   Bong et al PLoS One. 2016 Mar 28;11(3):e0152611. do

   Insert

   MTMR1 (MTMR1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Contains amino acids 95-607

   Promoter

   T7

   Available since

   Nov. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pOPINNUSA

   Plasmid

   #41119

   Depositor

   Ray Owens

   Article

   Bird et al Methods. 2011 Sep;55(1):29-37. Epub 2011

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Available since

   March 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDule-3-nitroTyrosine (A7)

   Plasmid

   #174078

   Purpose

   Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the third generation Mj 3NY (A7) synthetase and cognate amber suppressing tRNA in Ecoli.

   Depositor

   Ryan Mehl

   Article

   Beyer et al J Mol Biol. 2020 Jul 24;432(16):4690-470

   Insert

   3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Y32H H70T D158H I159A L162R

   Promoter

   GlnRS (constitutive)

   Available since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. p006-GFP-Logic-AND

   Plasmid

   #112237

   Purpose

   GFP from jellyfish Aquorea sp. under D61 AND gate-like ara+lac promoter with AraC repressor; lacI repressor; kanamycin resistant.

   Depositor

   Jaroslaw Bryk

   Article

   Siddall et al bioRxiv 2021.06.20.449138

   Inserts

   GFP under control of doubly-repressible promoter
   GFP
   araC
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   AND-gate and pLacI

   Available since

   Aug. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJDC181

   Plasmid

   #60853

   Purpose

   Expresses click beetle red luciferase

   Depositor

   Jeffrey Cirillo

   Article

   Chang et al PLoS One. 2014 Sep 29;9(9):e108341. doi:

   Insert

   Click beetle red luciferase (optimized for Mycobacteria)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   codon optimized for Mycobacteria

   Promoter

   hsp60

   Available since

   Nov. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. His-MBP-NRBF2

   Plasmid

   #99330

   Purpose

   Mammalian expression of NRBF2, which associates with the PI3KC3-C1 complex

   Depositor

   James Hurley

   Article

   Young et al Proc Natl Acad Sci U S A. 2016 Jul 19;11

   Insert

   Nuclear receptor-binding factor 2 (NRBF2 Human)
   

   Use

   Tags

   His-MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHH0103_PTPN22_Y-phosphatase

   Plasmid

   #110048

   Purpose

   Protein expression and purification of PTPN22_Y-phosphatase

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   PTPN22_Y-phosphatase (PTPN22 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCD185

   Plasmid

   #113317

   Purpose

   dCas9, MCP-SoxS_R93A

   Depositor

   Jesse Zalatan

   Article

   Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

   Insert

   dCas9 and MCP-SoxS_R93A
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   R93A

   Promoter

   Available since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. 6xHis-MBP-huPb2Cas12a

    Plasmid

    #174686

    Purpose

    Expresses 6xHis-MBP tagged Pb2Cas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huPb2Cas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFP.R377

    Plasmid

    #138247

    Purpose

    Expresses the split mCherry reporter interrupted by the Mja-KlbA intein (in cis)

    Depositor

    Baojun Wang

    Article

    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

    Inserts

    araC (araC )
    mCherryN(HDG).Mja-KlbA*.(CSG)mCherryC.H6
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    P(araBAD) and iPC

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. AcrIIA4

    Plasmid

    #101043

    Purpose

    Anti-CRISPRprotein which inhibit SpyCas9 and LmoCas9 activity.

    Depositor

    Zhiwei Huang

    Article

    Dong et al Nature. 2017 Jun 15;546(7658):436-439. d

    Insert

    AcrIIA4
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pYZ205

    Plasmid

    #104586

    Purpose

    Express SNAP-6His-CBD

    Depositor

    Yinhua Zhang, New England Biolabs

    Insert

    SNAP-6His-CBD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 4, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pET28a-SARS-CoV-2 NSP13

    Plasmid

    #176492

    Purpose

    Expresses the N-terminal His6-tagged SARS-CoV2 helicase (NSP13) in E coli.

    Depositor

    Xiaodong Cheng

    Article

    White et al J Phys Chem Lett. 2020 Nov 5;11(21):9144

    Insert

    SARS-CoV-2 NSP13 (ORF1ab SARS-CoV)
    

    Use

    Tags

    His6 and T7

    Expression

    Bacterial

    Mutation

    I570 from SARS-CoV NSP13 was mutated to V to conv…

    Promoter

    T7

    Available since

    Jan. 10, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. Tol2-lyzC-mcherry-2a-CDK2

    Plasmid

    #121131

    Purpose

    neutrophil specific expression

    Depositor

    Qing Deng

    Article

    Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

    Insert

    dre-WT CDK2 (cdk2 Zebrafish)
    

    Use

    Tags

    mcherry-2a

    Expression

    Bacterial

    Mutation

    Promoter

    neutrophil specific lyzC

    Available since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pOPINK-OTUD6A (full-length, aa 1-288)

    Plasmid

    #61416

    Purpose

    Expresses human OTUD6A (full-length) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD6A (OTUD6A Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GST-AR-624-919

    Plasmid

    #104200

    Purpose

    Expresses human AR-624-929 ligand binding domain in pGEX-5X-1 as GST fusion protein

    Depositor

    Elizabeth Wilson

    Article

    He et al Mol Cell Biol. 2003 Mar;23(6):2135-50.

    Insert

    human androgen receptor aa 624-919 (AR Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBLind

    Plasmid

    #113109

    Purpose

    Vector encoding the RFP gene under blue light-inducible promoter BindF1.

    Depositor

    Chueh Loo Poh

    Article

    Jayaraman et al ACS Synth Biol. 2018 Apr 20;7(4):986-994

    Insert

    RFP
    

    Use

    Cell-free

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BindF1

    Available since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 6375 H10Ydj1

    Plasmid

    #1234

    Depositor

    Susan Lindquist

    Article

    Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

    Insert

    Ydj1 (YDJ1 Budding Yeast)
    

    Use

    Tags

    his tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

20. pUC18T-mini-Tn7T-Tp-eyfp

    Plasmid

    #65035

    Purpose

    TpR mini-Tn7 vector, mobilizable, for YFP tagging bacteria

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Protoc. 2006;1(1):153-61.

    Insert

    eyfp
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge