31,065 results
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Plasmid#164588PurposeBacterial expression plasmid with sacB gene. Suitable for LIC cloning of a gene insert replacing the sacB.DepositorInsertTEV-SacB
TagsTrx-His6 followed by TEV siteExpressionBacterialPromoterT7Available SinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEXP5-NT/pT7 LuxI
Plasmid#193626PurposeConstitutive T7 RNAP-mediated expression of LuxI 3OC6-HSL synthase for Lux quorum sensing system. LuxI sequence is codon optimized for E. coli.DepositorInsertLuxI
Tags6xHisExpressionBacterialPromoterT7 RNAP promoterAvailable SinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAraGFPCDF
Plasmid#47516PurposeContains PBAD::GFP reporter, used for heterodimer competition assayDepositorInsertGFP
ExpressionBacterialAvailable SinceSept. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD deltahexa
Plasmid#169714PurposeBacterial Expression of human hnRNPA1-A Low complexity domain including amino acids 186-320, hexapeptide including aa 259-264 deletedDepositorInserthnRNPA1 (HNRNPA1 Human)
Tags6xHisExpressionBacterialMutationhnRNPA1-A Low complexity domain including amino a…PromoterT7Available SinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNIC- AVITAG-CTH10STII- -GG
Plasmid#215805PurposeCloning of target gene with C terminal non cleavable AVITAG and TEV cleavage of C terminal His10 and Twin Strep tagDepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET-6xHis-mCherry(-6)
Plasmid#205045PurposeFluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coliDepositorInsertmCherry(-6)
TagsMGHHHHHGGExpressionBacterialAvailable SinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDJSVT86
Plasmid#108965PurposeExpress periplasmic, outer membrane, or secreted proteins in E. coli - OmpA signal and 6His tagDepositorTypeEmpty backboneTagsOmpA 1-27, 6HisExpressionBacterialPromoterT7Available SinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJH645
Plasmid#172169PurposeReporter plasmid for pylBCD variant in pBAD backboneDepositorInsertPem7 luxCDA(B.1TAG)E; Plpp pylS; PproK pylT
ExpressionBacterialMutationInserted amber codon immediately after initiator …Available SinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNS20-SpCas9-SNAP
Plasmid#113717PurposeBacterial vector for expression of Snap-tagged Streptococcus pyogenes Cas9DepositorInsertCas9
UseCRISPRTagsHA-2xNLS-SNAP-NLS and His6-MBP-TEVExpressionBacterialAvailable SinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pM9-BetaGal-8C
Plasmid#172489PurposePlasmid encoding E. coli BetaGalactosidase with an N-terminal M9 nuclear localization sequenceDepositorInsertM9-BetaGal-8C
Tags6xHis and M9 nuclear localization sequence (from …ExpressionBacterialMutationBetaGal has two cysteine mutations (C77A, C1022S)…PromoterT7Available SinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNR276
Plasmid#79577PurposeTP901 attB-attP. GFP will express if sites recombine.DepositorInsertGFPmut3b
ExpressionBacterialAvailable SinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNR239
Plasmid#79576PurposeA118 attB-attP. GFP will express if sites recombine.DepositorInsertGFPmut3b
ExpressionBacterialAvailable SinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
AcrIIA4
Plasmid#101043PurposeAnti-CRISPRprotein which inhibit SpyCas9 and LmoCas9 activity.DepositorInsertAcrIIA4
TagsGSTExpressionBacterialAvailable SinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
HSPA9A
Plasmid#42469PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMH610
Plasmid#131862PurposeProduces holo-PhyB* in E. coliDepositorInsertsPhyB(1-651)-RGD-His6-Cys
HO1
PcyA
ExpressionBacterialPromoterT7 promoter-1 and T7 promoter-2Available SinceOct. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28-Dnmt3a3L-sc27
Plasmid#71827PurposeFor bacterial expression of Dnmt3a-Dnmt3L single-chain fusion protein protein. Contains the catalytic domain of mouse Dnmt3a and the C-terminal part of mouse Dnmt3L connected by 27 aa linker.DepositorInsertDnmt3a catalytic domain (M. musculus) Dnmt3L (M. musculus) C-terminal domain gene fusion (Dnmt3a Mouse)
Tags6xHis tagExpressionBacterialAvailable SinceJan. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMAL-p4X
Plasmid#75289PurposeExpresses proteins in the periplasm as fusions to a high-binding mutant (A313V) maltose-binding proteinDepositorTypeEmpty backboneTagsmaltose-binding proteinExpressionBacterialPromotertacAvailable SinceJuly 19, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEX-T-rnVAPA-1-125
Plasmid#13396DepositorAvailable SinceFeb. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSL0828 (pQCascade_crRNA-4)
Plasmid#130637PurposeExpresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array encodes a guide RNA that targets lacZ.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(target-4)
UseCRISPR; TransposonExpressionBacterialPromoterT7Available SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCasX1
Plasmid#87685Purposep15A plasmid expressing CasX locus from DeltaproteobacteriaDepositorInsertCasX1
ExpressionBacterialPromoterNoneAvailable SinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only