32,934 results

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pJMP3654

Plasmid

#222349

Purpose

Expression vector with GFP expressed under PabstBR promoter, kanR marker, and replicates in A. baumannii and E. coli.

Depositor

Jason Peters

Article

Bacon et al Microbiol Spectr. 2024 Nov 5;12(11):e013

Insert

sfGFP

Expression

Bacterial

Promoter

PabstBR

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pQFmcs-2H12.D11-scarREF

Plasmid

#221184

Purpose

cdGreen2 expressed from a cumate-inducible promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11240)

Depositor

Urs Jenal

Article

Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

Inserts

cdGreen2
mScarlet-I

Expression

Bacterial

Mutation

N-terminus changed: starts with MSKKYGEAVIKE ...

Promoter

None (same as cdGreen2) and PQ5 (cumate-inducible)

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pET28-2H12.D11

Plasmid

#221145

Purpose

His6-tagged cdGreen2 expressed from T7 promoter (Internal Lab ID: UJ11357)

Depositor

Urs Jenal

Article

Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

Insert

cdGreen2

Expression

Bacterial

Promoter

T7

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCDB179-HotPETase

Plasmid

#218701

Purpose

Encodes codon optimized HotPETase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

Insert

HotPETase

Tags

10xHis

Expression

Bacterial

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST17-His10-ccdB

Plasmid

#166145

Purpose

Gateway destination vector for generation of N-terminal His10-tagged proteins in E. coli.

Depositor

Daniel Jarosz

Article

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Type

Empty backbone

Tags

His10

Expression

Bacterial

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pTn7-SCOUT12.G

Plasmid

#201000

Purpose

pLv1-pUC18R6KT-miniTn7-Km-sfGFP, KmR, ApR

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Insert

superfolder GFP

Expression

Bacterial

Promoter

J23104

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pWH1266-Apra-sgRNA

Plasmid

#218372

Purpose

sgRNA expression plasmid for CRISPR-intereference

Depositor

Michael Gebhardt

Article

Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

Insert

sgRNA expression cassette

Use

CRISPR

Expression

Bacterial

Promoter

J23119

Available Since

May 2, 2024

Availability

Academic Institutions and Nonprofits only

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pNIC-HIS6-MBP-TEV-GG

Plasmid

#215800

Purpose

Cloning of target gene with N terminal His6 MBP tag and TEV Cleavage

Depositor

ASAP Discovery, Frank von Delft

Article

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Expression

Bacterial

Available Since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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Ecoli cpGFP

Plasmid

#215713

Purpose

Expresses cpGFP in Ecoli

Depositor

Katharine Diehl

Article

Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

Insert

cpGFP

Tags

6xHis

Expression

Bacterial

Mutation

circularly permuted GFP

Promoter

T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-tdMCP-24xGCN4

Plasmid

#205157

Purpose

express tdMCP-24xGCN4

Depositor

Weirui Ma

Article

Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

Insert

tdMCP-24xGCN4

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pET50_MDA5∆2CARD

Plasmid

#216804

Purpose

Expresses melanoma differentiation-associated protein 5 lacking its 2 CARD domains

Depositor

Sun Hur

Insert

melanoma differentiation-associated protein 5 delta 2 CARD domains (IFIH1 Human)

Expression

Bacterial

Mutation

∆1-287

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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OPEN HLA-A*02:01-BirA

Plasmid

#215569

Purpose

E. coli expression of BirA tagged HLA-A*02:01 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin.

Depositor

Nikolaos Sgourakis

Article

Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

Insert

OPEN HLA-A*02:01 (HLA-A Human)

Tags

BirA

Expression

Bacterial

Mutation

Glycine 120 to Cysteine

Promoter

T7

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pTcI39-switch

Plasmid

#195557

Purpose

Temperature state switch expressing low-temperature mWasabi and high-temperature mRFP1

Depositor

Mikhail Shapiro

Article

Xiong et al ACS Synth Biol. 2022 Jul 15;11(7):2518-2

Inserts

mRFP1
mWasabi
TcI39

Tags

6x His and AANDENYADAS degradation tag

Expression

Bacterial

Mutation

A67T

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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mTB3-BC2 nanobody

Plasmid

#210208

Purpose

Type 3 phage-display vector that contains kanamycin resistance and displays the BC2 nanobody at the N-terminus of mature protein III.

Depositor

Brian Kay

Article

Grahn et al Methods Mol Biol. 2023;2702:191-203. doi

Insert

BC2 Nanobody

Expression

Bacterial

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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COC053

Plasmid

#209131

Purpose

Enzymatic biotinylation of proteins at a specific lysine residue in a recognition sequence. This has multiple applications, especially in the study of protein-protein interactions.

Depositor

Christopher A. O'Callaghan

Article

O'callaghan et al Anal Biochem. 1999 Jan 1;266(1):9-15. do

Insert

Enzyme BirA (birA E. coli)

Expression

Bacterial

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a_Pro-IL-33

Plasmid

#214342

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL33 (IL33 Human)

Tags

His-TEV and Myc

Expression

Bacterial

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4.R_TRE_minTK

Plasmid

#211516

Purpose

Dual-luciferase reporter plasmid backbone for insertion of your TRE of interest driving minTK-luc2P expression in addition to constitutive SV-40-driven Renilla luciferase expression

Depositor

Justin English

Article

Zahm et al Nat Commun. 2024 Nov 28;15(1):10353. doi

Type

Empty backbone

Expression

Bacterial

Promoter

SV40 and minTK

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-6xHis-MBP-TEV site-TadA8e

Plasmid

#208319

Purpose

Overxpression MBP-TadA8e

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA8e

Expression

Bacterial

Mutation

mutations in TadA are described in the paper

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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WIW_Scaffold2

Plasmid

#205574

Purpose

Nanobody that inhibits seeding of tau fibrils

Depositor

David Eisenberg

Article

Abskharon et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

WIW_Scaffold2

Tags

6X His

Expression

Bacterial

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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HSA2_His

Plasmid

#202764

Purpose

human serum albumin design HSA2, in pET29b with C-terminal 6His-tag

Depositor

Sarel Fleishman

Article

Khersonsky et al J Mol Biol. 2023 Sep 1;435(17):168191. d

Insert

HSA2

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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