30,320 results

1. pTU1-A-pdh_RiboJ_mCherry_Bba_B0015

   Plasmid

   #107581

   Purpose

   B. megaterium DSM319 pdh promoter, mCherry (Bacillus codon optimised) for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)

   Depositor

   Paul Freemont

   Article

   Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p

   Insert

   mCherry
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pdh promoter Bacillus megaterium DSM319

   Available since

   April 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBbE8k-RFP

   Plasmid

   #35270

   Depositor

   Jay Keasling

   Article

   Lee et al J Biol Eng. 2011 Sep 20;5:12.

   Insert

   RFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   BAD

   Available since

   Feb. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. 2xNLS-pMJ915v2

   Plasmid

   #88916

   Purpose

   For expression of modified SpyCas9 in e.coli.

   Depositor

   Jennifer Doudna

   Article

   Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

   Inserts

   Cas9
   T7
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pETconNK

   Plasmid

   #81169

   Purpose

   pETconNK Empty Backbone

   Depositor

   Timothy Whitehead

   Article

   Klesmith et al Proc Natl Acad Sci U S A. 2017 Feb 14. p

   Type

   Empty backbone

   Use

   Tags

   c-myc

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   GAL1

   Available since

   Feb. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. zbFGF

   Plasmid

   #12309

   Depositor

   James Thomson

   Article

   Ludwig et al Nat Methods. 2006 Aug . 3(8):637-646.

   Insert

   Fgf2 (fgf2 Zebrafish)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Ala 11 mutated to Pro, Ser 55 mutated to Asn

   Promoter

   Available since

   Sept. 25, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. p007ampGFP

   Plasmid

   #58535

   Purpose

   GFP from jellyfish Aquorea sp. under T5 promoter with lac operators; ampicillin resistant.

   Depositor

   Jaroslaw Bryk

   Article

   Siddall et al bioRxiv 2021.06.20.449138

   Insert

   Green fluorescent protein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T5 between two lac operators

   Available since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET21-pelB-LaM-2

   Plasmid

   #172768

   Purpose

   Bacterial expression of anti-mCherry nanobody LaM-2, with pelB leader and C-term free cysteine and 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaM-2
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pL0M-S-mNeonGreen-EC18153

   Plasmid

   #137075

   Purpose

   Golden Gate Level 0 S module for N-terminal fluorescent protein tagging

   Depositor

   Julian Hibberd

   Article

   Luginbuehl et al Plant Direct. 2020 Feb 11;4(2):e00188. d

   Insert

   mNeonGreen DNA synthesis product with 5' and 3' extensions for Golden Gate cloning
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEND-193_Cacnes_HS

   Plasmid

   #225622

   Purpose

   C. acnes with heat shock inducible expression of pFAST. pBRESP36A with P(PPA_RS10245)+RBS_1+pFAST // P(PPA_RS06745)+RBS_1+HspR(PPA_RS10230)

   Depositor

   Marc Güell

   Article

   Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

   Insert

   HspR
   

   Use

   Synthetic Biology

   Tags

   pFAST-6XHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEND-229_Cacnes_EforCP

    Plasmid

    #225615

    Purpose

    Replicative vector for red fluorescent reporter protein eForCP strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+eForCP

    Depositor

    Marc Güell

    Article

    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

    Insert

    EforCP
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Codon optimized for Cutibacterium acnes

    Promoter

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. BPTI-[5,55]Gly14Ile38

    Plasmid

    #195059

    Purpose

    Thermal stability of BPTI

    Depositor

    Yutaka Kuroda

    Article

    Islam et al FEBS J. 2019 Oct;286(20):4122-4134. doi:

    Insert

    bovine pancreatic trypsin inhibitor
    

    Use

    Tags

    His tag and trpΔLE leader sequences

    Expression

    Bacterial

    Mutation

    Changed Alanine residues at position of 14 and 38…

    Promoter

    T7 promotor

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMSR:MSMEG_0835:Dendra

    Plasmid

    #174923

    Purpose

    C-term fluorescent fusion

    Depositor

    Joseph Wade, Keith Derbyshire

    Article

    Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

    Insert

    MSMEG_0835
    

    Use

    Integrating mycobacterial expression vector attl5…

    Tags

    Gly-Ala Linker/Dendra2/1x Flag Tag

    Expression

    Bacterial

    Mutation

    Promoter

    pmycTetO

    Available since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a-His-MinD_MinE

    Plasmid

    #133621

    Purpose

    Expression vector for the Escherichia coli MinD with an N-terminal His-tag for purification. MinE is encoded in the ORF to avoid toxicity when overexpressing MinD, but MinE is not tagged.

    Depositor

    Petra Schwille

    Article

    Loose et al Science. 2008 May 9;320(5877):789-92. do

    Insert

    His-MinD_MinE (minD, minE E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a(+)-SpdCas9-SBiT_Strep-Tag-II

    Plasmid

    #198750

    Purpose

    Expresses S. Pyogenes dCas9 fused to SmallBiT fragment of split NanoLuc luciferase

    Depositor

    Maarten Merkx

    Article

    van der Veer et al ACS Cent Sci. 2023 Mar 15;9(4):657-667.

    Insert

    SpdCas9 - SmallBiT
    

    Use

    Tags

    Strep-tag II

    Expression

    Bacterial

    Mutation

    D10A, H840A (nuclease inactivating mutations in &…

    Promoter

    T7

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKM263-USP7

    Plasmid

    #196365

    Purpose

    protein expression of the GST-tagged catalytic domain

    Depositor

    Stefan Knapp

    Insert

    USP7 catalytic domain
    

    Use

    Tags

    His6-GST-TEV

    Expression

    Bacterial

    Mutation

    contains only S207-E560

    Promoter

    T7

    Available since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. GB1-ALIX(P801G)

    Plasmid

    #180025

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(1-868, P801G). P801G mutation facilitates expression in E. coli.

    Depositor

    Lalit Deshmukh

    Article

    Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

    Insert

    GB1-His6-TEV-ALIX(1-868, P801G) (PDCD6IP Human)
    

    Use

    Tags

    GB1-His6-TEV

    Expression

    Bacterial

    Mutation

    P801G

    Promoter

    Available since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. E. coli expression: Z-lock cofilin dark state mutant

    Plasmid

    #141135

    Purpose

    a control for optogenetic cofilin analog

    Depositor

    Klaus Hahn

    Article

    Stone et al Nat Chem Biol. 2019 Dec;15(12):1183-1190

    Insert

    Zdk2, Cofilin, LOV2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D3 deletion, I32F in Zdk2

    Promoter

    Available since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJDC60

    Plasmid

    #157780

    Purpose

    tdTomato is expressed under the mycobacterial L5 promoter for in vivo and in vitro detection.

    Depositor

    Jeffrey Cirillo

    Article

    Kong et al Methods Mol Biol. 2018;1790:75-85. doi:

    Insert

    tdTomato
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    L5

    Available since

    Sept. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKB083

    Plasmid

    #181952

    Purpose

    IPTG-inducible expression of PhoQ

    Depositor

    Jeffrey Tabor

    Article

    Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

    Insert

    PhoQ; lacI (lacI PhoQ - Salmonella enterica subsp. enterica serovar Typhimurium; lacI - E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PhoQ-Ptac; lacI-PlacIq

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pF709-pET28a-Hs-FL-ETFbeta-NHis wt

    Plasmid

    #85110

    Purpose

    expression of recombinant human full-length ETFbeta wt with N-terminal 6xHis-tag

    Depositor

    Pal Falnes

    Article

    Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

    Insert

    human full-length ETFbeta (ETFB Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge