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pH6HTC_TKT-Halo

Plasmid

#175331

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

TKT (TKT Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-p40PX(aa1-148)

Plasmid

#19008

Depositor

Michael Yaffe

Article

Kanai et al Nat Cell Biol. 2001 Jul . 3(7):675-8.

Insert

p40phox (NCF4 Human)

Tags

GST

Expression

Bacterial

Mutation

aa1-148 (PX domains)

Available Since

Aug. 25, 2008

Availability

Academic Institutions and Nonprofits only
pUNIV-EGFP

Plasmid

#24706

Depositor

Cynthia Czajkowski

Article

Venkatachalan et al Pflugers Arch. 2007 Apr . 454(1):155-63.

Insert

EGFP (eGFP Aequoria victoria)

Expression

Bacterial, Mammalian, and Y…

Available Since

Dec. 6, 2010

Availability

Academic Institutions and Nonprofits only

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pCheY Tc delta C

Plasmid

#13520

Depositor

James McKerrow

Article

Eakin et al J Biol Chem. 1993 Mar 25. 268(9):6115-8.

Insert

cruzain

Tags

CheY

Expression

Bacterial

Mutation

Truncated variant: stop codon after Gly212

Available Since

March 11, 2009

Availability

Academic Institutions and Nonprofits only
pHV739

Plasmid

#117477

Purpose

Expression of NMT2

Depositor

Richard Kahn

Article

Van Valkenburgh et al Methods Enzymol. 2002;344:186-93.

Insert

NMT2 (NMT2 Human)

Expression

Bacterial

Available Since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pSR1051

Plasmid

#208739

Purpose

Characterization plasmid for PZwf (sfgfp expressed from PZwf on the S. aureus pC194 replicon)

Depositor

Lauren Andrews

Article

Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021

Insert

PZwf

Use

Synthetic Biology

Expression

Bacterial

Promoter

PZwf

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pSR1057

Plasmid

#208742

Purpose

Characterization plasmid for PPgk (sfgfp expressed from PPgk on the S. aureus pC194 replicon)

Depositor

Lauren Andrews

Article

Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021

Insert

PPgk

Use

Synthetic Biology

Expression

Bacterial

Promoter

PPgk

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pETM33_CRK_SH3

Plasmid

#178472

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

CRK_SH3 (CRK Human, Synthetic)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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GST-PS-Gads 1-155 GB1 Gads 261-330-Cys (XSB365)

Plasmid

#78507

Purpose

Construct for expressing Gads SH3, SH2, and SH3 domain in E. Coli, with a C-terminal Cys for labeling

Depositor

Ron Vale

Article

Su et al Science. 2016 Apr 29;352(6285):595-9. do

Insert

Gads SH3 SH2 SH3 domain (GRAP2 Human)

Tags

GST followed by a prescission protease claevage s…

Expression

Bacterial

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_HSPA9_HSP70

Plasmid

#109776

Purpose

Protein expression and purification of HSPA9_HSP70

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

HSPA9_HSP70 (HSPA9 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pQTEV-LOC57019

Plasmid

#34811

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

cytokine induced apoptosis inhibitor 1 (CIAPIN1 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pSCB2-sgRNA

Plasmid

#129463

Purpose

Template plasmid for guide sequence insertion via inverse PCR. Derived from pSCrhaB2-gRNA (see paper).

Depositor

Silvia Cardona

Article

Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

Insert

pgRNA

Use

CRISPR

Expression

Bacterial

Promoter

BBa_J23119 (SpeI)

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pGEM p21

Plasmid

#8443

Depositor

Frederic Mushinski

Article

Huppi et al Oncogene 1994 Oct;9(10):3017-20.

Insert

Mouse p21 (Cdkn1a Mouse)

Expression

Bacterial

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
His6_MBP_FXR2P_P474S_P493N

Plasmid

#198695

Purpose

Expression of human FXR2 protein in E. coli

Depositor

Simpson Joseph

Article

Edwards et al Sci Rep. 2020 Sep 28;10(1):15858. doi: 1

Insert

FXR2P (FXR2 Human)

Tags

MBP

Expression

Bacterial

Mutation

Changed proline 474 to serine and proline 493 asp…

Promoter

T7

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pJUMP29-1E(sfGFP)

Plasmid

#126982

Purpose

Level 1 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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XP7 Sonic hh

Plasmid

#16983

Depositor

Randall Moon

Insert

sonic hedgehog

Expression

Bacterial

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pECTO

Plasmid

#67683

Purpose

pMAD2 derivative designed for chromosomal gene ectopic complementation in Staphylococcus aureus

Depositor

Philippe Bouloc

Type

Empty backbone

Expression

Bacterial

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX-2T-Cdc42-wt

Plasmid

#12969

Depositor

Gary Bokoch

Insert

Cdc42 (CDC42 Human)

Tags

GST

Expression

Bacterial

Available Since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only
pkk223_Null

Plasmid

#213111

Purpose

pkk223 backbone plasmid

Depositor

Martin Horvath

Article

Utzman et al PLoS One. 2024 May 8;19(5):e0284642. doi

Type

Empty backbone

Expression

Bacterial

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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18 pCOLI_A_Trx

Plasmid

#160255

Purpose

N-term His-Thioredoxin 3C cleavable Amp resistant bacterial expression vector

Depositor

John Weir

Article

Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

Type

Empty backbone

Tags

6xHis and Thioredoxin

Expression

Bacterial

Promoter

T7

Available Since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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