31,059 results
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Plasmid#185724PurposeDirected evolution of Cas9 adenine base editors with altered PAM compatibilityDepositorInsertecTadA(8e)-gp41-8N
UseCRISPRExpressionBacterialMutationecTadA(8e) R26GAvailable SinceSept. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBpaRS3tRNA
Plasmid#197102PurposeTo express the p-benzoylphenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into p-benzoylphenylalanine.DepositorInsertTyrRS variant, amber suppressor tRNA
ExpressionBacterialMutationGly32, Ser107, Thr158, Ser159, Arg286 (TyrRS)Available SinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_NXF1_TAP-C
Plasmid#110038PurposeProtein expression and purification of NXF1_TAP-CDepositorInsertNXF1_TAP-C (NXF1 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a T7pCONS sfGFP
Plasmid#154462PurposepET28a with a design flaw in the T7 promoter fixed. Contains the coding sequence for sfGFPDepositorInsertsuper folder GFP
TagsHis-thrombinExpressionBacterialPromoterT7Available SinceAug. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)::NL
Plasmid#141289PurposeNLUC in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)DepositorInsertNLUC
UseLuciferaseExpressionBacterialMutationEngineered for high stability (t1/2 = 11.5 days a…Promoterpromoter for bacteriophage T7 RNA polymeraseAvailable SinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
YL-CashGem-BAT1-URA3-LINEAR
Plasmid#174837PurposepRS414 backbone containing LINEAR fragment targeting BAT1 in Y. lipolyticaDepositorInsertCodon optimized Cas9:hGem
UseCRISPR and Synthetic BiologyTagshGeminin tagExpressionBacterial and YeastPromoterGPDpAvailable SinceJan. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBbA5c-RFP
Plasmid#35281DepositorInsertRFP
UseSynthetic BiologyExpressionBacterialPromoterlacUV5Available SinceFeb. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTCF5 HLA-A*01:01
Plasmid#180448PurposeE. coli expression of soluble MHC proteinDepositorInsertHLA-A*01:01
TagsBSP41ExpressionBacterialMutationReplacement of transmembrane domain with enzymati…Available SinceFeb. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDawn-Ag43
Plasmid#107742PurposeExpresses Ag43 under the control of a blue-light transcriptional regulatorDepositorInsertAg43 (flu )
ExpressionBacterialMutationremoved all pstI cut sites with silent mutations,…PromoterpLambdaAvailable SinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
1a. pETM11-SUMO-SNCA-GFP
Plasmid#107292PurposePlasmid for the expression of the construct alpha-Synuclein-GFP, which contains N-terminal tags, which can be cleaved later: His-tag for purification and SUMO-tag for better solubilityDepositorAvailable SinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only