30,327 results

1. pHH0103_FYN-1/1

   Plasmid

   #91413

   Purpose

   Protein expression and purification of human SH3 domain construct FYN-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   FYN-1/1 (FYN Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. EID1-pGex-2TK

   Plasmid

   #22689

   Depositor

   William Kaelin

   Article

   Miyake et al Mol Cell Biol. 2000 Dec . 20(23):8889-90

   Insert

   E1A-like inhibitor of differentiation 1 (EID1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   wt cDNA

   Promoter

   Available since

   Dec. 3, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pB3.P11

   Plasmid

   #69577

   Purpose

   OT-I TCR beta chain cDNA in pCRScript

   Depositor

   Francis R. Carbone

   Article

   Hogquist et al Cell. 1994 Jan 14;76(1):17-27.

   Insert

   TCR beta cDNA clone B3 (Tcrb Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET30b-7Y-SrtA

   Plasmid

   #105601

   Purpose

   expresses 7Y sortase A variant in E.coli

   Depositor

   Stephanie Dougan

   Article

   Jeong et al PLoS One. 2017 Dec 4;12(12):e0189068. do

   Insert

   S.aureus Sortase A, 7Y mutant
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGT-B1

   Plasmid

   #122579

   Purpose

   MAGIC donor plasmid (pBBR1 oriR with beta-lactamase/sfGFP payload)

   Depositor

   Harris Wang

   Article

   Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

   Insert

   sfGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJDC182

   Plasmid

   #60854

   Purpose

   Expresses firefly luciferase

   Depositor

   Jeffrey Cirillo

   Article

   Chang et al PLoS One. 2014 Sep 29;9(9):e108341. doi:

   Insert

   Firefly luciferase (optimized for Mycobacteria)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   codon optimized for Mycobacteria

   Promoter

   hsp60

   Available since

   Nov. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGTM_COV2_NSP5_C145A_001_SUMO

   Plasmid

   #192349

   Purpose

   Expresses SARS-CoV-2 3CLpro with an active site mutation (C154A) for the expression of the mature protease in an inactive form

   Depositor

   Gaetano Montelione

   Article

   Mazzei et al STAR Protoc. 2023 May 5;4(2):102326. doi

   Insert

   SARS-COV-2 Mpro-C145A (NEWENTRY SARS-CoV-2)
   

   Use

   Tags

   6X-His-Sumo

   Expression

   Bacterial

   Mutation

   Cysteine 145 of the active site is substituted wi…

   Promoter

   T7 lac

   Available since

   June 3, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. Lenti_EGFP-P2A-HAUS6_IRES_Blast

   Plasmid

   #182886

   Purpose

   Transfer vector for production of lentivirus. Expresses EGFP-P2A-HAUS6 (WT full length)

   Depositor

   Daniel Gerlich

   Article

   David et al J Cell Biol. 2019 Jul 1;218(7):2150-2168

   Insert

   HAUS 6 (HAUS6 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   EF1alpha core

   Available since

   July 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDD87

   Plasmid

   #119892

   Purpose

   The pLPD03031 promoter from Rhodococcus opacus PD630 expressing GFP+ on the pAL5000/pMB1 backbone with kanamycin resistance.

   Depositor

   Tae Seok Moon

   Article

   DeLorenzo et al ACS Synth Biol. 2017 Oct 20;6(10):1973-1

   Insert

   pLPD03031_GFP+
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. MP5

    Plasmid

    #69677

    Purpose

    Untargetted Bacterial Mutagenesis

    Depositor

    David Liu

    Article

    Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

    Insert

    dnaQ926 dam seqA ugi CDA1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    E. coli PBAD

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHYRSF1

    Plasmid

    #34549

    Depositor

    Hideo Iwai

    Article

    Muona et al Nat Protoc. 2010 . 5(3):574-87.

    Insert

    DnaE N-intein
    

    Use

    Tags

    H6-GB1

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. bdSUMO-ZF5.3-GFP-HiBit

    Plasmid

    #170992

    Purpose

    For bacterial expression of bdSUMO tagged muGFP with ZF5.3 peptide and NanoLuc complementation peptide variant #86

    Depositor

    Angus Johnston

    Article

    Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

    Insert

    14x-His-bdSUMO-muGFP-HiBiT
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTpal-fdc

    Plasmid

    #78288

    Purpose

    Expresses A. thaliana PAL2 and S. cerevisiae FDC1 in E. coli

    Depositor

    David Nielsen

    Article

    McKenna et al Biotechnol J. 2013 Dec;8(12):1465-75. do

    Inserts

    PAL2 (PAL2 Mustard Weed)
    FDC1 (FDC1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    *see below

    Promoter

    trc

    Available since

    June 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEN-dCas9-CDA1-UGI

    Plasmid

    #196248

    Purpose

    Plasmid containing promoter of RecA from Xanthomonas oryzae driving expression of dead Cas9, cytosine deaminase CDA1, and glycosylase inhibitor

    Depositor

    Bing Yang

    Article

    Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

    Insert

    attL4-RecApro-dCas9-CDA1-UGI-attR1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    RecA promoter

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHH0103_BCAR1-1/1

    Plasmid

    #91418

    Purpose

    Protein expression and purification of human SH3 domain construct BCAR1-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    BCAR1-1/1 (BCAR1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pETM11-6xHis-TEV-hORF1p_K3AK4A

    Plasmid

    #202585

    Purpose

    Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the K3A and K4A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scar

    Depositor

    Liam Holt

    Article

    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

    Insert

    ORF1p
    

    Use

    Tags

    6x His and TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    Changed ORF1p Lysine 3 to Alanine, changed ORF1p …

    Promoter

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pALlR1

    Plasmid

    #172566

    Purpose

    Inducible gene expression, pACYC origin, LacI repressor, PLlacO-1 promoter, mRFP, Chloramphenicol Resistance Marker.

    Depositor

    Christopher Reisch

    Insert

    LacI-mRFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLlacO-1

    Available since

    Nov. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. Gfp-rt-vasa

    Plasmid

    #167322

    Purpose

    Plasmid for synthesis of GFP chimeric RNA that contained vasa-3'UTRs of rainbow trout by in vitro transcription.

    Depositor

    Goro Yoshizaki

    Article

    Yoshizaki et al Biol Reprod. 2005 Jul;73(1):88-93. doi:

    Insert

    rt vasa 3'UTRs (ddx4 rainbow trout)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJYP1

    Plasmid

    #202406

    Purpose

    Backbone vector to clone toxic or unstable DNA using dual-control (transcriptional control via naringenin inducible pfdeA promoter - translational control via B12 riboswitch)

    Depositor

    Remy Loris

    Article

    Vandierendonck et al Toxins (Basel). 2023 Aug 18;15(8):508. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    fdeA

    Available since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET_BGL1

    Plasmid

    #101910

    Purpose

    Expresses a β-glucosidase gene from Thermomicrobium roseum in E. coli under the control of the pET system.

    Depositor

    Steve Shih

    Article

    Husser et al ACS Synth Biol. 2018 Mar 16;7(3):933-944

    Insert

    β-glucosidase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7+LacO

    Available since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge