30,808 results
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Plasmid#156482PurposeBacterial expression of Sars-CoV2 Nsp16 protein with His-tag and GST-tagDepositorInsertNsp16 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
TagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKL004
Plasmid#98920PurposeConstitutively expresses the calcium sensor GCaMP6f tethered to mRuby3 under the Sigma 70 promoter in E. coli.DepositorInsertGCaMP6f tethered to mRuby
TagsHis and mRuby3ExpressionBacterialPromoterBBa_J23118 - Strong ConstitutiveAvailable SinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEYN12-mflon
Plasmid#165581PurposeLon protease from Mesoplasma florumDepositorInsertlon protease
ExpressionBacterialPromoterPtrcAvailable SinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
NylB'_pET21b
Plasmid#215416PurposeExpression construct for NylB' gene, codon optimized for expression in E. coliDepositorInsertNylB'
ExpressionBacterialAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP3_Sap
Plasmid#206849PurposeA plasmid containing a 17,271 bp fragment of the P. tricornutum chloroplast genome. The yeast marker URA3 was domesticated for SapI and integrated into the chloroplast fragment.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP2
Plasmid#206847PurposeA plasmid containing a 15,249 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP1
Plasmid#206846PurposeA plasmid containing a 14,519 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPANI2A2E2-Crimson
Plasmid#193758PurposeExpresses E2-Crimson under the control of PANI2 synthetic promoter, ColE1 origin of replication, Ampicillin selectionDepositorInsertE2-Crimson
UseSynthetic BiologyExpressionBacterialPromoterPANI2 synthetic promoter carrying binding sites f…Available SinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDUAL_tacI
Plasmid#155170PurposeBaSP and CuCBP co-expression: tacI promoter controls BaSP expressionDepositorInsertsSucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda
UseSynthetic BiologyTagsHis Tag and Strep - Tag IIExpressionBacterialAvailable SinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET sfGFP(150TAG)
Plasmid#133455Purposeexpresses sfGFP with an unnatural amino acid at the 150th position in bacterial cellsDepositorInsertsfGFP
TagsHisExpressionBacterialPromoterT7Available SinceNov. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET26b_Nb.AT110.i1
Plasmid#135481PurposeE. coli periplasmic expression of high-affinity conformational nanobody Nb.AT110.i1 recognizing angiotensin II type 1 receptorDepositorInsertNanobody AT110.i1
Tags6xHis-tag and pelB leaderExpressionBacterialPromoterT7Available SinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pASR103
Plasmid#122637PurposeBglFusion compatible IPTG inducible integration vector for Streptococcus pneumoniae (pPEPZ Backbone)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceAug. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNR188
Plasmid#79543PurposeOutput plasmid: contains 16-state register made up of TP901, BxbI, and A118 recognition sites.DepositorTypeEmpty backboneExpressionBacterialAvailable SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSMT3-FBF2
Plasmid#166972PurposeExpresses FBF-2 in E. coli for purification and crystallization studiesDepositorInsertFBF-2 (fbf-2 Nematode)
TagsHis-SUMOExpressionBacterialMutationFBF-2 PUM RNA binding domain; amino acids 164-575PromoterT7Available SinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
Rac1
Plasmid#55692PurposeExpression of mouse Rac1DepositorAvailable SinceSept. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRSET-B CFP
Plasmid#108858PurposeEncodes for the fluorescent protein CFPDepositorInsertCFP
ExpressionBacterialAvailable SinceApril 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEB2_mScarletI_mVenusNB-MrkH
Plasmid#182291Purposeexpresses c-di-GMP biosensor in E. coliDepositorInsertmVenusNB-MrkH
TagsmVenusNB internal fusion to MrkHExpressionBacterialPromoterproCAvailable SinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
RALGDS
Plasmid#25331PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertRALGDS:N793-F914 (RALGDS Human)
TagsHisExpressionBacterialMutationContains amino acids N793-F914Available SinceSept. 23, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pENTR1A-SynTetOff-GFP
Plasmid#190230PurposeGateway entry vector, high-level expression of EGFP in neuronal cellsDepositorInsertEGFP
ExpressionBacterialAvailable SinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP-(SIM)3
Plasmid#126955PurposeBacterial expression plasmid for eGFP-(SIM)3 client to assess SUMO-SIM scaffolds.DepositorInsertEGFP-SUMO interacting motif (SIM) from PIASx (PIAS2 Human)
TagseGFPExpressionBacterialPromoterunknownAvailable SinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only