34,238 results
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Plasmid#128732PurposeGateway destination low-copy number plasmid for expression of any effector from avrPto promoter with C-terminal Cya tagDepositorTypeEmpty backboneExpressionBacterialAvailable sinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only
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pYC1876
Plasmid#158722PurposeHelper plasmid pYC1640 for C-terminal tagging with degradation tag ssrADepositorInsertssrA
UseCRISPRExpressionBacterialAvailable sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
NLS-tdTomato
Plasmid#163443PurposeBacterial expression of His-tagged, SV40 NLS-tagged tdTomato in the modified pRSETB protein expression backboneDepositorInserttdTomato
Tags6xHisExpressionBacterialPromoterT7Available sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G06_ZIKV NS5
Plasmid#145358PurposeCell-free expression vector for Zika virus NS5 protein with C-term mCherryDepositorInsertZIKV NS5
TagsmCherry and 8xHisExpressionBacterialPromoterT7Available sinceNov. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET26b_Nb.AD101
Plasmid#131402PurposeE. coli periplasmic expression of conformational nanobody Nb.AD101 recognizing purified active-state A2a adenosine receptorDepositorInsertNanobody AD101
Tags6xHis-tag and pelB leaderExpressionBacterialPromoterT7Available sinceNov. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
8 pCOLI_A_GST
Plasmid#160150PurposeN-term GST 3C cleavable Amp resistant bacterial expression vectorDepositorTypeEmpty backboneExpressionBacterialPromoterT7Available sinceNov. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GNAI1_G-alpha
Plasmid#110012PurposeProtein expression and purification of GNAI1_G-alphaDepositorInsertGNAI1_G-alpha (GNAI1 Human)
ExpressionBacterialAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ACTG2_Actin_2
Plasmid#109990PurposeProtein expression and purification of ACTG2_Actin_2DepositorInsertACTG2_Actin_2 (ACTG2 Human)
ExpressionBacterialAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_EEF1A1_GTP-EFTU
Plasmid#109861PurposeProtein expression and purification of EEF1A1_GTP-EFTUDepositorInsertEEF1A1_GTP-EFTU (EEF1A1 Human)
ExpressionBacterialAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_RANBP1_Ran-BP1
Plasmid#109872PurposeProtein expression and purification of RANBP1_Ran-BP1DepositorInsertRANBP1_Ran-BP1 (RANBP1 Human)
ExpressionBacterialAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAG308-His-redFAST
Plasmid#160350PurposeExpresses His-tagged redFAST in bacteriaDepositorInsertHis-redFAST
ExpressionBacterialAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
T7 promoter + rpsL reporter plasmid
Plasmid#156455PurposeA mutational reporter plasmid that can gain Streptomycin and/or Tetracycline resistance upon mutation. Contains T7 promoter.DepositorInsertsrpsL
TcR
ExpressionBacterialMutationATG start codon mutated to ACGPromotertac and tetAvailable sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
R717-X61-566: His6-MBP-tev-Hs.RAF1(52-192)
Plasmid#159687PurposeE. coli protein expression of His6-MBP-tev-Hs.RAF1(52-192)DepositorAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKL11-GCaMP6f bb100
Plasmid#158983PurposeConstitutive expression (sigma 70 promoter) of GCaMP6f fluorophore in E. coli.DepositorInsertGCaMP6f
ExpressionBacterialMutationcodon optimized for e. coli from Addgene#100833PromoterBba_J23100 (sigma 70)Available sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAH18
Plasmid#154397PurposeDerived from pGPI-SceI; Trimethoprim resistant; carries I-SceI gene; contains 475 bp from both upstream and downstream flanking regions between the FRT sites on pAHCTX1-rhadCas9DepositorInsertFused upstream and downstream homologous regions around FRT sites when pAH-CTX1-rhadCas9 integrated into K56-2 genome
UseSynthetic BiologyExpressionBacterialPromoterNAAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET28b-XI
Plasmid#157988PurposeExpresses XI in E.coli cellDepositorInsertxylose isomerase
ExpressionBacterialAvailable sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET-DUET-1-mRhubarb719-HO
Plasmid#141200PurposeExpresses mRhubarb719 fluorescent protein and heme oxygenaseDepositorInsertsmRhubarb719
heme oxygenase
Tags6x-HisExpressionBacterialMutationR134H, Y198S, T202Y, V203I, W309R, Q310A compared…PromoterT7Available sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHis-LMW_FGF2
Plasmid#157657PurposeExpresses FGF2 in E.coli cellDepositorInsertFibroblast growth factor 2 (FGF2 Human)
TagsHis tagExpressionBacterialMutationchanged Cystein 211,229 to serine, deleted amino …Available sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
mUAVbla:tetR 5′
Plasmid#149379PurposemUAVbla containing a tetracycline resistance (tetA) cassette for building Gene Doctoring donor plasmids from pDOC-GG, using BsaI Golden Gate assembly with GGAG and CGCT overhangsDepositorInserttetA
UseSynthetic BiologyExpressionBacterialAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pASPIre2
Plasmid#154843PurposeDerivative of pASPIre1 carrying a bxb1-sfGFP translational fusion in place of bxb1DepositorInsertBxb1-sfGFP fusion controlled by rhamnose-inducible promoter; attB/attP-flanked discriminator containing mCherry; rhaRS operon
ExpressionBacterialAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only