34,238 results
-
Plasmid#133228PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with PsNifH (pOGG043), gusA (pOGG083) and T-pharma (pOGG003)
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable sinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOPS0262
Plasmid#133227PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with PsNifH (pOGG043), celB (pOGG050) and T-pharma (pOGG003)
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R270
Plasmid#138219PurposeExpresses the split mCherry reporter interrupted by the IMPDH-1 intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R272
Plasmid#138221PurposeExpresses the split mCherry reporter interrupted by the SspGyrB artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET30b_T7Sav
Plasmid#138588PurposeT7-tagged streptavidin with native C-terminus controlled by PT7 promoter; KanR, pBR322 oriDepositorInsertT7-tagged streptavidin variant with native C-terminus
UseSynthetic BiologyTagsT7-tagExpressionBacterialPromoterPT7Available sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJM160
Plasmid#134169PurposeBacterial expression of RB3 stathmin-like domain (SLD)DepositorAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
GST-RNF126
Plasmid#138643PurposeBacterial expression of GST-RNF126 fusion proteinDepositorAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pNCST-AausFP2
Plasmid#129500PurposeExpresses AausFP2 constitutively in E. coli (most strains)DepositorInsertAausFP2
ExpressionBacterialPromotersynthetic constitutive (stationary phase) promote…Available sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM061
Plasmid#134174PurposeBacterial expression of GST-HJURPDepositorAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-3-SH3BP1
Plasmid#136647PurposeExpression of SHBP1-GST fusion protein in E. coliDepositorInsertSH3BP1 (SH3BP1 Human)
ExpressionBacterialAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MinC
Plasmid#133628PurposeExpression vector for the Escherichia coli MinC with an N-terminal fusion of a His-tag for purification.DepositorAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mCherry-2xEcMTS
Plasmid#133624PurposeExpression vector for mCherry fused to a tandem repeat of the E. coli MinD membrane targeting sequence (MTS) (C-terminal fusion) and an N-terminal His-tag for purification.DepositorInsertmCherry-2xEcMTS
TagsHisExpressionBacterialAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGGB_RT_tdTomato_li
Plasmid#136964PurposeGoldengate/Greengate N-tag module, containing tdTomato fused to a flexible linkerDepositorInserttandem Tomato
ExpressionBacterialAvailable sinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGGA_RT_pLexA
Plasmid#136961PurposeGoldengate/Greengate module for generation of beta-estradiole inducible genes.DepositorInsertpLexA
ExpressionBacterialAvailable sinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
-
pB1H2 UV5 Zif268-5ala (2-hybrid bait plasmid)
Plasmid#128165PurposeUV5 promoter drives expression of Zif268-5alanine peptide fusion. Pair with pGHUC w-ERBIN as a negative control (doesn't turn on HIS3/GFP).DepositorInsert10 amino acid glycine/serine linker + 5alanines
UseSynthetic BiologyTagsFLAG and zif268ExpressionBacterialPromoterUV5Available sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mRFP1-kanMX6
Plasmid#133551PurposeYeast fluorescent (RFP) vector with PTEF promoter.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCA24N_6xHis-TEV-rpsA
Plasmid#83585PurposeExpress E. coli ribosomal protein S1 with a TEV-cleavable, N-terminal His tagDepositorAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJYScr
Plasmid#132719PurposePlasmid for an easy-to-construct crRNA delivery vector using hybridized oligonucleotides. Constitutive transcription of crRNA, Spectinomycin resistanceDepositorTypeEmpty backboneUseCRISPRExpressionBacterialAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
KRigor980
Plasmid#129763PurposeExpresses full-length Drosophila Kinesin-1 with rigor mutation (R210A) for adhering microtubules to surfacesDepositorAvailable sinceNov. 18, 2019AvailabilityAcademic Institutions and Nonprofits only