31,826 results
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Plasmid#237239Purposeover-express E. coli hk/P-dnaN (Pol III beta sliding clamp subunit, N-terminal cleveable his-kinase tag)DepositorInsertdnaN (beta), N-terminal his-kinase cleavable tag (dnaN E. coli)
Tagshis-10+PKA kinase motif+PreScission Protease clea…ExpressionBacterialAvailable SinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBWB514
Plasmid#209326Purposep15A-tetR-tet-SapI-ME-RBS-mRFP-ME-U1-Barcode-U2-SapI-dbI terminator-oriT-Cm. Fragment expression vector with Golden Gate sites.DepositorInsertmRFP
ExpressionBacterialPromotertetAvailable SinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBEL2319
Plasmid#195697PurposeCryo-EM of 6xHis-Foldon-(Gly-Ser-Linker)-MlaCDepositorInsertFoldon-MlaC
ExpressionBacterialMutation6xHis-Foldon-(Gly-Ser-Linker)-MlaCAvailable SinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pET 15b-UbcH5a/2417
Plasmid#11472DepositorAvailable SinceApril 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pTAT2.1
Plasmid#35620DepositorTypeEmpty backboneTags6xHIS and TATExpressionBacterialPromoterT7Available SinceMarch 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDonor14-ShoHELIX-KanR (BO4)
Plasmid#181795PurposepDonor for ShoHELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence and encoding a kanamycin resistance gene as cargoDepositorInsertKanR, R6K origin of replication
ExpressionBacterialAvailable SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GRB2-1/2
Plasmid#91480PurposeProtein expression and purification of human SH3 domain construct GRB2-1/2DepositorInsertGRB2-1/2 (GRB2 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEXP5-NT/pLas eGFP
Plasmid#193630PurposeInducible expression of eGFP by the transcription factor complex LasR-3OC12 homoserine lactone (HSL).DepositorInserteGFP
Tags6xHisExpressionBacterialPromoterT7 RNAP promoterAvailable SinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGGF_RT_FAST_Venus
Plasmid#136980PurposeGoldengate/Greengate module marker for transgenic Arabidopsis seed coat fluorescenceDepositorInsertseed coat marker, replaced RFP w/ Venus. Replaced NOS terminator w/ Arabidopsis HSP18-2 terminator
ExpressionBacterialMutationBsaI site domestication in pOLE1:OLE1-RFP 688 G &…Available SinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
Nsp3 Mac1 - P43 (Oxford)
Plasmid#169210PurposeExpression of 6xHis tagged protein for crystallization, includes TEV protease site for tag removalDepositorInsertSARS-CoV-2 Nsp3 Mac1
Tags6xHisExpressionBacterialPromoterT7Available SinceJuly 28, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28b-pchG WT
Plasmid#87107Purposeexpresses PchG WT, the thiazolinyl imine reductase from Pseudomonas aeruginosaDepositorInsertpchG WT
Tagshis-tagExpressionBacterialPromoterT7Available SinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSNAP-CLC-EYFP
Plasmid#38011DepositorInsertClathrin, light polypeptide (LCa) (Clta Mouse)
TagsEYFP and SNAP26mExpressionBacterial and MammalianPromoterCMVAvailable SinceSept. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pmSod1-WT-N’His
Plasmid#117701PurposeExpression of WT mouse SOD1 with N- terminal His tagDepositorAvailable SinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQC
Plasmid#221134PurposeContains [PibpA-gfpASV] and [PcpxPmcherryASV]OPT genetic modules in the pSEVA631(Spec) backbone, separated by the secG leuU terminatorDepositorInserts[PibpA-gfpASV]
[PcpxPmcherryASV] OPT
ExpressionBacterialPromotercpxP and ibpAAvailable SinceJune 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
Human RNF144B (27-236)
Plasmid#110753PurposeBacterial Expression of RNF144B RBR domainDepositorAvailable SinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEA01
Plasmid#170766PurposeUtilisation of pSAM2 excision tool in Amycolatopsis sppDepositorInsertsxis, int
oriT
pac
ExpressionBacterialPromotertrcpAvailable SinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
p403 – TDH3-MTS-mCherry-GFP1-10-His
Plasmid#177730PurposemCherry and the 1-10th β-strands of GFP (GFP1-10) fused to a mitochondria signal sequence for mitochondrial targeting in yeast plasmid with HIS markerDepositorInsertmCherry-GFP1-10
TagsMitochondrial Targeting SequenceExpressionBacterial and YeastAvailable SinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNBU2_erm_P1T_DP-GH023
Plasmid#90317PurposeIntegration vector at an attB site in Bacteroides; contains the P1T_DP aTC-inducible promoter with the RBS GH023DepositorTypeEmpty backboneExpressionBacterialAvailable SinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTXBI-mclover-YTHDC1-FL
Plasmid#177121PurposeBacterial expression of Full-length YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purificationDepositorInsertYTHDC1 (YTHDC1 Human)
TagsMxe GyrA intein-Chitin binding domain for protein…ExpressionBacterialPromoterT7Available SinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only