33,454 results

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WT-eGFP

Plasmid

#215168

Purpose

Encodes the enhanced green fluorescent protein

Depositor

Travis Beddoe, Bhuvana Shanbhag

Insert

WT-eGFP

Tags

His-tag

Expression

Bacterial

Promoter

T7 lac

Available since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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His-GST

Plasmid

#215169

Purpose

Encodes the synthetic glutathionine S-transferase with N-terminal His tag

Depositor

Travis Beddoe, Bhuvana Shanbhag

Insert

His-GST

Tags

His-tag

Expression

Bacterial

Promoter

T7 lac

Available since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGTM_COV2_NSP5_004_SUMO

Plasmid

#190062

Purpose

Expresses SARS-CoV-2 3CLpro for the expression of the active mature protease

Depositor

Gaetano Montelione

Publication

Mazzei et al STAR Protoc. 2023 May 5;4(2):102326. doi

Insert

3CLpro (ORF1ab SARS-CoV-2)

Tags

6X-His-Sumo

Expression

Bacterial

Promoter

T7 lac

Available since

June 3, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pDmREDusk

Plasmid

#213701

Purpose

Vector for red-light-repressed bacterial gene expression

Depositor

Andreas Moeglich

Publication

Meier et al Nat Commun. 2024 Jun 10;15(1):4876. doi:

Type

Empty backbone

Expression

Bacterial

Available since

May 30, 2024

Availability

Academic Institutions and Nonprofits only

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PE-SUMO CRYAB

Plasmid

#217670

Purpose

For expression of SUMO tagged human alpha B crystallin

Depositor

Larry David

Publication

Halverson-Kolkind et al Exp Eye Res. 2024 Nov;248:110115. doi: 1

Insert

CRYAB (CRYAB Human)

Tags

6XHis and Smt3

Expression

Bacterial

Available since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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pET-29b(+)_NSP4ss_SerM_NucA-myc

Plasmid

#218191

Purpose

Bacterial expression of a periplasmically expressed myc-tagged Serratia Marcescens NucA (Benzonase)

Depositor

Florence Pojer

Publication

Rieubon et al protocols.io 2026. doi: https://dx.doi.o

Insert

nucA

Tags

2xmyc and NSP4 signal secretion peptide

Expression

Bacterial

Mutation

codon optimized for E. coli, residues 22-266

Promoter

T7

Available since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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pTn7-SCOUT11.G

Plasmid

#200999

Purpose

pLv1-pUC18R6KT-miniTn7-Gm-sfGFP, GmR, ApR

Depositor

Philip Poole

Publication

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Insert

superfolder GFP

Expression

Bacterial

Promoter

J23104

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-hph-Ptet2

Plasmid

#218368

Purpose

Empty backbone for Tn7-site insertion with tetR-PtetA expression system with two tetR operator sites

Depositor

Michael Gebhardt

Publication

Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

Type

Empty backbone

Expression

Bacterial

Available since

May 2, 2024

Availability

Academic Institutions and Nonprofits only

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Luciferase

Plasmid

#213979

Purpose

Expresses Doxy Inducible Luciferase

Depositor

Coleman Lindsley

Publication

Reilly et al Blood. 2021 Sep 9;138(10):898-911. doi:

Insert

Luciferase

Use

Lentiviral

Tags

6xHis affinity tag

Expression

Bacterial and Mammalian

Promoter

Tet-responsive promoter PTight, consisting of sev…

Available since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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NylCA-TS_pET28a

Plasmid

#215419

Purpose

Expression construct for NylCA-TS gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Publication

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

NylCA-TS

Expression

Bacterial

Mutation

S111G/A137L/E263Q

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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FsC_pET29b

Plasmid

#215405

Purpose

Expression construct for FsC gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Publication

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

FsC

Expression

Bacterial

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pPbU6_hdhfr/yfcu_Cas9

Plasmid

#216423

Purpose

Empty backbone to express gene specific gRNA from the Plasmodium berghei U6 promoter and the Cas9 nuclease for traditional CRISPR editing.

Depositor

Ellen Bushell

Publication

Jonsdottir et al bioRxiv 2024.04.20.590404

Type

Empty backbone

Use

CRISPR; Expression in plasmodium berghei

Expression

Bacterial

Available since

April 28, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Spacer Switch Plasmid

Plasmid

#215346

Purpose

Facilitates the change in spacer sequence to Neisseria gonorrhoeae encoding the Nla CRISPRi system.

Depositor

Hank Seifert

Publication

Geslewitz et al mBio. 2023 Dec 21:e0302523. doi: 10.1128

Insert

OpaD spacer; Chloramphenicol Resistance Cassette

Use

CRISPR

Expression

Bacterial

Available since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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Zika NS5 RdRp

Plasmid

#204788

Purpose

Bacterial expression of Zika NS5 RdRp

Depositor

ASAP Discovery, Frank von Delft

Insert

Zika NS5 RdRp domain

Expression

Bacterial

Available since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MBP-C5E-6XTUBE

Plasmid

#217628

Purpose

Bacterial expression of MBP tagged 6XTUBE

Depositor

Ethan Lee

Publication

Kassel et al Sci Signal. 2023 Feb 7;16(771):eabn8372.

Insert

6XTUBE

Expression

Bacterial

Available since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pBroad-T7RNAP-apraR

Plasmid

#199993

Purpose

T7 RNAP circuit variant T15 on ultra broad host range vector (RSF1010 and pAMB1 origins) apraA

Depositor

Farren Isaacs

Publication

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

T7RNAP Circuit

Expression

Bacterial

Mutation

copFnull

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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OPEN Beta2-microglobulin

Plasmid

#215657

Purpose

E. coli expression of human beta-2-microglobulin containing a cysteine mutation for disulfide linkage to mutant HLA.

Depositor

Nikolaos Sgourakis

Publication

Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

Insert

OPEN Beta2-microglobulin (B2M Human)

Expression

Bacterial

Mutation

Histidine 31 to Cysteine

Promoter

T7

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pColdI-GST-mGTSF1

Plasmid

#214671

Purpose

Bacterial expression of GST-tagged mouse GTSF1

Depositor

Phillip Zamore

Publication

Arif et al Nature. 2022 Aug;608(7923):618-625. doi:

Insert

GTSF1 (Gtsf1 Mouse)

Tags

6x His and GST

Expression

Bacterial

Promoter

CspA

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pFN18A-HaloTag-Biotin

Plasmid

#206039

Purpose

HaloTag-(Ig32)2-Talin R3-IVVI-(Ig32)2-10xHisTag-AviTag

Depositor

Sergi Garcia-Manyes

Publication

Tapia-Rojo et al Nat Protoc. 2024 Mar 11. doi: 10.1038/s4

Insert

Talin R3-IVVI (Tln1 Mouse)

Tags

10 x His-AviTag and Halo-Tag

Expression

Bacterial

Mutation

Threonine 809 to Isoleucine, Threonine 833 to Val…

Promoter

T7

Available since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSWEET-bgaB

Plasmid

#210272

Purpose

Xylose-dependent expression system for tight and modulated expression of cloned genes in Bacillus subtilis.

Depositor

Eric Brown

Publication

Bhavsar et al Appl Environ Microbiol. 2001 Jan;67(1):4

Insert

bgaB

Expression

Bacterial

Promoter

PxylA

Available since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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