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PE-SUMO CRYAB

Plasmid

#217670

Purpose

For expression of SUMO tagged human alpha B crystallin

Depositor

Larry David

Article

Halverson-Kolkind et al Exp Eye Res. 2024 Nov;248:110115. doi: 1

Insert

CRYAB (CRYAB Human)

Tags

6XHis and Smt3

Expression

Bacterial

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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pETMutS

Plasmid

#13245

Depositor

Peggy Hsieh

Article

Biswas et al J Biol Chem 1996 Mar 1;271(9):5040-8.

Insert

MutS

Expression

Bacterial

Mutation

None

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pUCP20T-ecfp

Plasmid

#78463

Purpose

Broad range bacterial fluorescence labeling

Depositor

Mariette Barbier

Article

Barbier et al PLoS One. 2016 Mar 3;11(3):e0146827. doi

Insert

eCFP

Expression

Bacterial

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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pAG GST EGFP

Plasmid

#236736

Purpose

Bacterial expression plasmid containing GST-tagged EGFP

Depositor

Melina Fan, Addgene Research Program

Insert

EGFP

Tags

GST

Expression

Bacterial

Promoter

Tac

Available Since

April 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pDEST-hisMBP-AsCpf1-EC

Plasmid

#79007

Purpose

Expressing his-MBP tagged AsCpf1 (E.coli codon optimized)

Depositor

Jin-Soo Kim

Article

Hur et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

Insert

AsCpf1

Tags

6xHis-MBP-NLS and NLS-HA

Expression

Bacterial

Promoter

tac promoter

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pSJX016

Plasmid

#232324

Purpose

Genome editing in Caulobacter crescentus

Depositor

Wei Zhao

Article

Sun et al Nucleic Acids Res. 2025 Apr 22;53(8):gka

Inserts

sgRNA
SpCas9M

Use

CRISPR

Tags

H-arms and sfGFP

Expression

Bacterial

Promoter

PJ23119 and Pvan

Available Since

June 16, 2025

Availability

Academic Institutions and Nonprofits only

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pMGE-T7

Plasmid

#153021

Purpose

Single-plasmid multi-gene expression system. Each genes under the control of a T7 and the lacO promoter sequences.

Depositor

Vito Valiante

Article

Kufs et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Promoter

T7

Available Since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

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pET28b(+)_rPD-L1nb

Plasmid

#226715

Purpose

Heterologous expression and purification of recombinant PD-L1 nanobody (rPD-L1nb)

Depositor

Gautam Dantas

Article

Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

Insert

Recombinant PD-L1 nanobody

Tags

HA tag and Histidine tag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pDest-521

Plasmid

#159688

Purpose

Gateway destination vector for E. coli protein expression

Depositor

Dominic Esposito

Article

Tran et al Nat Commun. 2021 Feb 19;12(1):1176. doi:

Type

Empty backbone

Expression

Bacterial

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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p2H12Matry

Plasmid

#221143

Purpose

cdGreen-Matry expressed from an aTc-inducible promoter (Internal Lab ID: UJ11208)

Depositor

Urs Jenal

Article

Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

Insert

cdGreen-Matry

Expression

Bacterial

Promoter

Ptet

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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