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32,947 results

Showing: 7561 - 7580 of 32947 results

  1. pAcHELIX-sgRNA_entry (CJT94)

    Plasmid
    #181783
    Purpose
    Expresses AcHELIX containing a nicking I-AniI fusion to AcTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-AcTnsB, AcTnsC, AcTniQ, AcCas12k
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available Since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    181783-plasmid-map-sequence-id-358223

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  5. pET-NlaCascade-NLS-6xHis

    Plasmid
    #180213
    Purpose
    Expressing Neisseria lactamica Type I-C Cascade subunits (Cas5-Cas8-Cas11-Cas7) with NLS and His tag on the C terminus of Cas7 for protein purification in E.Coli
    Depositor
    Yan Zhang
    Article
    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.
    Insert
    NlaCas5-Cas8-Cas7
    Tags
    6XHis and NLS
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    180213-plasmid-map-sequence-id-356902

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  8. pNLS-2xBFP(4C)

    Plasmid
    #176151
    Purpose
    Encodes NLS-2xBFP(4C), which is a tandem BFP with an N-terminal SV40 NLS. The protein has 4 solvent accessible cysteines for labeling with maleimide dyes.
    Depositor
    Siegfried Musser
    Article
    Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d
    Insert
    NLS-2xBFP(4C)
    Tags
    6xHis tag and SV40 nuclear localization sequence …
    Expression
    Bacterial
    Mutation
    Compared with EGFP, the two BFP domains have the …
    Promoter
    Ptrc
    Available Since
    March 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    176151-plasmid-map-sequence-id-356068

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  9. pGEX-5X-1 U1-C-FL

    Plasmid
    #129077
    Purpose
    generation of GST-tagged U1-C protein using bacteria
    Depositor
    Takeshi Tsubata
    Insert
    U1-C (SNRPC Human)
    Expression
    Bacterial
    Available Since
    March 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    129077-plasmid-map-sequence-id-252125

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  11. PRDX1A-c001

    Plasmid
    #175473
    Purpose
    Protein expression in bacterial cells. Entire CDS, M1-K199. Can be biotinylated.
    Depositor
    Opher Gileadi
    Insert
    PRDX1 (PRDX1 Human)
    Tags
    AviTag and His6-TEV
    Expression
    Bacterial
    Available Since
    Jan. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    175473-plasmid-map-sequence-id-353180

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  12. EPB41L3A-c000

    Plasmid
    #175458
    Purpose
    Protein expression in bacterial cells. Entire CDS, M1-D1087.
    Depositor
    Opher Gileadi
    Insert
    EPB41L3 (EPB41L3 Human)
    Tags
    His6-TEV
    Expression
    Bacterial
    Available Since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175458-plasmid-map-sequence-id-353141

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  15. pATZ-BFP-HA-GFP11

    Plasmid
    #177721
    Purpose
    ER protein alpha-1-antitrypsin containing E342K mutation (ATZ mutant) fused to blue fluorescence protein (BFP) tag protein, an HA tag and the eleventh β-strand of GFP
    Depositor
    Erik Böttger
    Article
    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:
    Insert
    alpha-1-antitrypsin (SERPINA1 Human)
    Tags
    GFP11, HA, and TagBFP
    Expression
    Bacterial and Mammalian
    Mutation
    E342K (ATZ mutant)
    Available Since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    177721-plasmid-map-sequence-id-352621

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  18. pUltra-5HTP

    Plasmid
    #178042
    Purpose
    Express modified ScTrpRS/tRNA for genetic incorporation of 5HTP to TAG codon
    Depositor
    Han Xiao
    Article
    Chen et al Chem. 2020 Oct 8;6(10):2717-2727. doi: 1
    Inserts
    Modified ScTrpRS for 5HTP incoporation
    suppressor tRNA for 5HTP incorporation
    Expression
    Bacterial
    Mutation
    T107C, P254T, C255A
    Available Since
    Jan. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    178042-plasmid-map-sequence-id-352270

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Showing: 7561 - 7580 of 32947 results