34,238 results
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Plasmid#233886PurposepRSF-DUET1 for protein overexpression of human BRD4DepositorAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only
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pRSET-iGluSnFR4s
Plasmid#234443PurposeBacterial expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow)DepositorInsertiGluSnFR4s
Tags6xHis-Xpress epiExpressionBacterialPromoterT7Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCV019-catP
Plasmid#232825PurposeRandom transposon mutagenesis vector for Bacteroidales species - chloramphenicolDepositorInsertcatP
ExpressionBacterialAvailable sinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
KPC2-pNic-Bsa4
Plasmid#231082Purposeexpresses Klebsiella pneumoniae carbapenemase-2 (KPC-2) under T7 with His tagDepositorAvailable sinceFeb. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEND-184_Cacnes_mCherry
Plasmid#225610PurposeReplicative vector for red fluorescent reporter protein mCherry strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+mCherry.DepositorInsertmCherry
UseSynthetic BiologyTagsV5-6xHisExpressionBacterialMutationCodon optimized for Cutibacterium acnesAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET22b-T7-6h-GST-α-Synuclein
Plasmid#225224PurposeAlpha synuclein gene fused with gst gene under t7 promoter for bacterial expression of alpha synuclein protein.DepositorTags6xHis Tag and TEV Cleavage SiteExpressionBacterialPromoterT7Available sinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTec_mCherry
Plasmid#229145PurposePromoter strength evaluation of PtecDepositorInsertmCherry
ExpressionBacterialMutationNoPromoterPtecAvailable sinceJan. 29, 2025AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET-30a-L6KD-PT linker-PEP-Mtu ΔI-CM-xylanase
Plasmid#231128PurposeExpresses xylanase via a cSAT2.0 schemeDepositorInsertxylanase
TagscSAT2.0ExpressionBacterialPromoterT7Available sinceJan. 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVP141
Plasmid#222079PurposeSingle component CRISPR-mediated base-editor for Agrobacterium genetic engineering visually marked with sfGFP for guide insertion.DepositorInsertdCas9-PmCDA1, sfGFP and sacB
UseCRISPRExpressionBacterialAvailable sinceJan. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG94-15
Plasmid#228971PurposeFor bacterial expression of anti-GFP nanobody LaG94-15, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.DepositorInsertLaG94-15
Tags6xHISExpressionBacterialPromoterT7Available sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pOPINB-M1-di-Ub(G76V)-HOIL-1
Plasmid#229539PurposeConstitutively active M1-di-ubiquitin HOIL-1 fusion protein for expression in E. coliDepositorTags6xHis-3CExpressionBacterialMutationChanged Gly 76 to Val and changed Gly 76 to ValPromoterT7Available sinceDec. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCN38-yfp
Plasmid#227637PurposeNegative control plasmid encoding yfp downstream of an attenuated ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and chloramphenicol resistance to S. aureus.DepositorInsertyfp
ExpressionBacterialAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKJ0463
Plasmid#228715PurposeBacterial expression of epT7 RNAP; for protein production and purificationDepositorPublicationInsertSumo_epT7
ExpressionBacterialMutationT3M/G47A/E643GAvailable sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDFDuet-AtUBC8-S-AtUBA1-S
Plasmid#222714PurposeExpression AtUBA1-S and AtUBC8-S in in bacterial cellsDepositorAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-parkin
Plasmid#227367PurposeFor bacterial expression of parkinDepositorInsertParkin (PRKN Human)
TagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression.Available sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCF1135
Plasmid#222106PurposeE. coli - Veillonella shuttle plasmid with single selectable markerDepositorTypeEmpty backboneUseE. coli-veillonella shuttle plasmidExpressionBacterialAvailable sinceOct. 31, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCF1148
Plasmid#222330PurposeFluorescent reporter plasmid for VeillonellaDepositorInsertbs2 gene with Veillonella parvula SKV38 mdh promoter
UseE. coli-veillonella shuttle plasmidExpressionBacterialPromoterVeillonella parvula SKV38 mdh promoterAvailable sinceOct. 31, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
WT USP7 catalytic
Plasmid#227201PurposeExpresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
Tags6HisExpressionBacterialMutationaa 207-560PromoterT7-promoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDIV272
Plasmid#226183PurposeExpresses Cas9 and gRNA targeting uidA gene of the pDIV643 derived cassette.DepositorInserttRNA-sgRNA-tRNA
UseCRISPRExpressionBacterial and YeastPromoterTEF1p (Komagataella phaffii)Available sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD_FLAG-GFP(Y39TAG)-6His
Plasmid#223510PurposeFluorescent reporter plasmid for genetic code expansion which consists of a modified GFP with a Amber stop codon on position Y39DepositorInsertGFP(Y39TAG)
Tags6xHis and FLAGExpressionBacterialMutationY39TAGAvailable sinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only