30,844 results

1. pHN1_lacUV5-mNG-PDT

   Plasmid

   #165583

   Purpose

   Expresses mNeonGreen and C-terminally fused PDT

   Depositor

   Daniel Ducat

   Article

   Sakkos et al ACS Synth Biol. 2021 Jul 16;10(7):1667-1

   Insert

   mNeonGreen-PDT
   

   Tags

   Protein degradation tag

   Expression

   Bacterial

   Promoter

   Ptrc

   Available Since

   Nov. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pC011 LwCas13a locus with targeting spacer

   Plasmid

   #91903

   Purpose

   LwCas13a locus into pACYC184 with targeting spacer

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

   Insert

   LwCas13a with targeting spacer
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Oct. 16, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pAD6

   Plasmid

   #134349

   Purpose

   E. coli mfd gene cloned into pSKB2 with an N-terminal, ppx-cleavable His6 tag

   Depositor

   Seth Darst

   Article

   Deaconescu et al Acta Crystallogr Sect F Struct Biol Crys

   Insert

   mfd (mfd E. coli)
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. DDX3XA

   Plasmid

   #42362

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   DDX3XA (DDX3X Human)
   

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   See Comments

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pQE80L-SH3

   Plasmid

   #160331

   Purpose

   This plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its thermostability and quantum yield is improved compared to wild type.

   Depositor

   Sang Taek Jung

   Article

   Ko et al J Agric Food Chem. 2019 Oct 30;67(43):12

   Insert

   SH3
   

   Tags

   6xHis tag

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJMP12049

   Plasmid

   #222355

   Purpose

   Tn7 integrative vector with GFP expressed under PabstBR promoter and aprR marker on Tn7 transposon.

   Depositor

   Jason Peters

   Insert

   sfGFP
   

   Expression

   Bacterial

   Promoter

   PabstBR

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEB1-mGFPmut3

   Plasmid

   #103981

   Purpose

   Plasmid encoding mGFPmut3

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   mGFPmut3
   

   Use

   Low copy

   Expression

   Bacterial

   Mutation

   Mutations relative to wild-type GFP (S65G, S72A, …

   Promoter

   proC

   Available Since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET15b LFn-Fla

   Plasmid

   #84871

   Purpose

   Bacterial expression of LFn-Fla fusion

   Depositor

   Russell Vance

   Article

   Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

   Inserts

   LFn
   Fla
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pET22b-CPDSacI

   Plasmid

   #38255

   Depositor

   Matthew Bogyo, Aimee Shen

   Article

   Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

   Type

   Empty backbone

   Tags

   6xHis and Vibrio cholerae MARTX toxin cysteine pr…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET His6 ProteinG TEV LIC cloning vector (2P-T)

    Plasmid

    #29713

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    His6-ProteinG-TEV

    Expression

    Bacterial

    Available Since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. B4-1

    Plasmid

    #111475

    Purpose

    Ala-tRNA synthetase

    Depositor

    Cheemeng Tan

    Article

    Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

    Insert

    Ala-tRNA synthetase
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    June 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. Tensin-2 WT

    Plasmid

    #201797

    Purpose

    Expresses wild-type Tensin-2 protein in MAC-Tag-C backbone

    Depositor

    Vesa Hytönen

    Article

    Turkki et al Sci Rep. 2024 Aug 27;14(1):19862. doi: 1

    Insert

    Tensin-2 (Tns2 Mouse)
    

    Tags

    BirA, HA, strepIII

    Expression

    Bacterial and Mammalian

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTE5400_pMBP_CDS dropout vector

    Plasmid

    #221189

    Purpose

    Expression vector for N-terminally His-tagged maltose binding protein (10xHis-MBP) fused to a protein of interest

    Depositor

    Tobias Erb

    Article

    Barthel et al Synth Biol (Oxf). 2024 Sep 20;9(1):ysae0

    Type

    Empty backbone

    Tags

    10x His-tag + E. coli maltose binding protein (MB…

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJCC_083 Cas12i1

    Plasmid

    #171670

    Purpose

    For bacterial expression of Cas12i1 with an N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

    Insert

    Cas12i1
    

    Tags

    10X His, TEV protease cleavage site, and maltose-…

    Expression

    Bacterial

    Available Since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFA-DAB2

    Plasmid

    #162690

    Purpose

    Expresses DabAB2 inorganic carbon transport complex

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    DabAB2 inorganic carbon transport complex
    

    Expression

    Bacterial

    Promoter

    PLtet0-1 promoter

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. His-GIV-CTs

    Plasmid

    #69864

    Purpose

    Express His tagged GIV-CTs (1660–1870).

    Depositor

    Pradipta Ghosh

    Article

    Garcia-Marcos et al Mol Biol Cell. 2011 Mar 1;22(5):673-86.

    Insert

    GIV/Girdin (CCDC88A Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    C terminal amino acids S1660-S1870 of human GIV.

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. Aurein1.2_(+36GFP)-Cre

    Plasmid

    #71748

    Purpose

    Aurein1.2+36GFP-Cre fusion expression in E. Coli

    Depositor

    David Liu

    Article

    Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

    Insert

    (GGS)9_Aurein1.2+36GFP-Cre
    

    Tags

    6XHis

    Expression

    Bacterial

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRSFDuet-1_VNp15-StefinA

    Plasmid

    #182405

    Purpose

    Bacterial expression of Vesicle Nucleating peptide15-stefinA fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp15-stefin (CSTA Human)
    

    Tags

    Vesicle Nucleating peptide 15 (VNp15)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET_RFP-SZ2

    Plasmid

    #124933

    Purpose

    Expression of RFP-SZ2

    Depositor

    Matthew Good, Daniel Hammer, Benjamin Schuster

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    RFP-SYNZIP2
    

    Tags

    His and mCherry

    Expression

    Bacterial

    Mutation

    SZ2 domain

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pIYN3

    Plasmid

    #197099

    Purpose

    To express the variant of M. jannaschii TyrRS specific for halogenated tyrosines and Nap3 amber suppressor tRNA and allow UAG codon to be translated into these derivatives.

    Depositor

    Kensaku Sakamoto

    Article

    Mukai et al Biochem Biophys Res Commun. 2011 Aug 12;

    Insert

    TyrRS variant, Nap3 tRNA
    

    Expression

    Bacterial

    Mutation

    Ala70, Thr158, Ser159, Arg286 (TyrRS)

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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