30,176 results
-
Plasmid#196974PurposeBacterial expression plasmid for GST-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal mNeonGreen tag and cleavable N-terminal GST tagDepositorInsertKinesin Heavy Chain (Khc Fly)
UseTagsGST and mNeonGreenExpressionBacterialMutationPromotertacAvailable sinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEB2-mRuby3 Addgene
Plasmid#104005PurposePlasmid encoding mRuby3 AddgeneDepositorInsertmRuby3 Addgene
UseLow copyTagsExpressionBacterialMutationMutations relative to eqFP611 (MSKGEE LIKENMRMKVV…PromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX_hDDX5_FL_D248N
Plasmid#88872Purposeinducible expression of GST-DDX5 D248N in bacteriaDepositorInsertDDX5 (DDX5 Human)
UseTagsGSTExpressionBacterialMutationD248NPromoterTacAvailable sinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
FEV_pet28a
Plasmid#131668PurposeBacterial expression of ETS gene FEVDepositorInsertFEV (FEV Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJUMP46-2A(sfGFP)
Plasmid#126990PurposeLevel 2 vector. sfGFP reporter. Origin p15A (medium copy number)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJuly 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3 I0500 LuxI
Plasmid#107965PurposeArabinose-inducible 3OC6HSL sender plasmidDepositorInsertluxI
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJ211_WT_low
Plasmid#128848PurposeLow copy number plasmid for expression of E. coli flagellin from its natural promoterDepositorInsertFlagellin
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1 EGFP-Sumo2 GG
Plasmid#13384DepositorInsertSumo2 GG (SUMO2 Human)
UseTagsEGFP and GSTExpressionBacterialMutationMature SUMO2GG: diglycine at amino acid positions…PromoterAvailable sinceNov. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBSV2_2
Plasmid#118226PurposeEmpty E. coli - B. burgdorferi shuttle vector; kanamycin resistantDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMSP2
Plasmid#26282DepositorInsertMSP2
UseTags6-HisExpressionBacterialMutationsynthetic gene of dimer of deletion mutant (1-43)…PromoterAvailable sinceApril 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
p006kanGFP
Plasmid#58534PurposeGFP from jellyfish Aquorea sp. under T5 promoter with lac operators; kanamycin resistant.DepositorInsertGreen fluorescent protein
UseTagsExpressionBacterialMutationPromoterT5 between two lac operatorsAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pQE60-Exp5
Plasmid#12553DepositorInsertEXPORTIN-5 (XPO5 Human)
UseTagsHISExpressionBacterialMutationNONEPromoterAvailable sinceOct. 2, 2006AvailabilityAcademic Institutions and Nonprofits only -
pnEA-vH_His-TEV-SEPT2-sfCherry2_SEPT6
Plasmid#180313Purposebacterial co-expression of human SEPT2 fused to superfolder Cherry2 and of human SEPT6DepositorUseTagsHis6-TEV and sfCherry2ExpressionBacterialMutationPromoterAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pK
Plasmid#64188Purposeharbours marker gene LYS2DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAug. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAB107a
Plasmid#79218PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI
UseTagsExpressionBacterialMutationPromoterPlacZ-opt (OR1); Ppro1Available sinceMay 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFnCpf1GG
Plasmid#131010PurposeBackbone plasmid for generating CRISPR arrays for FnCas12a. Contains a GFP-dropout cassette and a direct repeat.DepositorInsertsdirect repeat of FnCas12a
GFP expression cassette
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTYB11-PTBP1-RRM1
Plasmid#89153PurposeRecombinant expression of PTBP1-RRM1 in E.coliDepositorInsertPolypyrimidine Tract Binding Protein 1 RNA Recognition Motif 1 (PTBP1 Human)
UseTagsIntein and chitin binding domainExpressionBacterialMutationPromoterT7Available sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
-
cryS96
Plasmid#67519PurposeαB-crystallin minipeptide fused to ELP (S96).DepositorInsertmini-αB-crystallin fused to ELP S96 (VPGSG) (CRYAB Human, Synthetic)
UseTagselastin-like polypeptide (ELP) S96ExpressionBacterialMutationmini-αB-crystallin contains residues 73–92 of aB-…PromoterT7Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1(6CNW)RS
Plasmid#222873PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 6-Cyano-L-Tryptophan (6-CN-Trp) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
UseTagsExpressionBacterialMutationPromoterT7Available sinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only