30,844 results

1. pET21b-Caspase-3(D3A,V266E)

   Plasmid

   #90091

   Purpose

   Expresses caspase D3A and V266E mutant in bacterial cells

   Depositor

   Clay Clark

   Article

   Pop et al Biochemistry. 2003 Oct 28;42(42):12311-2

   Insert

   Caspase 3 (CASP3 Human)
   

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   D175A D28A D9A V266E

   Available Since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAB168

   Plasmid

   #101671

   Purpose

   Opto-T7RNAP*(563-F1), equal expr.

   Depositor

   Mustafa Khammash

   Article

   Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

   Insert

   Opto-T7RNAP*(563-F1), equal expr.
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Sept. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. (SUMO)5-(SIM)10

   Plasmid

   #126923

   Purpose

   Bacterial expression plasmid for multivalent scaffold of (SUMO)5-(SIM)10 biomolecular condensates to recruit SUMO-containing client proteins.

   Depositor

   Michael Rosen

   Article

   Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

   Insert

   5 repeats of human SUMO3 and 10 repeats of human SUMO interactive motif (SIM) from PIASx each separated by (GGS)4 (SUMO3 Human)
   

   Expression

   Bacterial

   Mutation

   internal BamHI site between (SUMO)5 and (SIM)10

   Promoter

   tac

   Available Since

   Sept. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRSFDuet-FBXL5-SKP1

   Plasmid

   #226620

   Purpose

   Co-expression of FBXL5-SKP1 complex in E.coli

   Depositor

   Ning Zheng

   Article

   Wang et al Mol Cell. 2020 Apr 2;78(1):31-41.e5. doi

   Inserts

   FBXL5 (FBXL5 Human)
   SKP1 (SKP1 Human)
   

   Tags

   GB1

   Expression

   Bacterial

   Mutation

   Amino acids 1-198 was deleted, and 420-596 was re…

   Promoter

   T7

   Available Since

   Oct. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. TRBV12-3

   Plasmid

   #123445

   Purpose

   construction of TCR

   Depositor

   Catherine Wu

   Article

   Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

   Insert

   TRBV12-3 (TRBV12-3 Human)
   

   Expression

   Bacterial

   Mutation

   WT

   Available Since

   May 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. TRBV7-6

   Plasmid

   #123434

   Purpose

   construction of TCR

   Depositor

   Catherine Wu

   Article

   Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

   Insert

   TRBV7-6 (TRBV7-6 Human)
   

   Expression

   Bacterial

   Mutation

   WT

   Available Since

   May 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. TRBV30

   Plasmid

   #123460

   Purpose

   construction of TCR

   Depositor

   Catherine Wu

   Article

   Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

   Insert

   TRBV30 (TRBV30 Human)
   

   Expression

   Bacterial

   Mutation

   WT

   Available Since

   May 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. TRAV34

   Plasmid

   #123402

   Purpose

   construction of TCR

   Depositor

   Catherine Wu

   Article

   Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

   Insert

   TRAV34 (TRAV34 Human)
   

   Expression

   Bacterial

   Mutation

   WT

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. TRAV6

   Plasmid

   #123373

   Purpose

   construction of TCR

   Depositor

   Catherine Wu

   Article

   Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

   Insert

   TRAV6 (TRAV6 Human)
   

   Expression

   Bacterial

   Mutation

   WT

   Available Since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TRAV3

    Plasmid

    #123370

    Purpose

    construction of TCR

    Depositor

    Catherine Wu

    Article

    Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

    Insert

    TRAV3 (TRAV3 Human)
    

    Expression

    Bacterial

    Mutation

    WT

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCVL.Active/Repressed TLR (Sce)

    Plasmid

    #45575

    Purpose

    Codes for the Active/Repressed TLR with the target site for I-Sce I nuclease

    Depositor

    Andrew Scharenberg

    Article

    Kuhar et al Nucleic Acids Res. 2013 Oct 10.

    Inserts

    eGFP with I-Sce I TS
    +3 mCherry
    

    Use

    Lentiviral

    Tags

    T2A dislinker and iRFP

    Expression

    Bacterial and Mammalian

    Mutation

    embedded I-Sce I TS from 163-185

    Promoter

    SFFV

    Available Since

    Nov. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. M58 pPET28b-TEV-full-length zebrafish beta catenin

    Plasmid

    #17199

    Depositor

    Randall Moon

    Insert

    beta catenin (ctnnb1 Zebrafish)
    

    Tags

    HIS

    Expression

    Bacterial

    Available Since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

13. pAL-pck_NT

    Plasmid

    #74069

    Purpose

    pAL374 plasmid carrying the pck (NT) sgRNA, targeting the non-template strand of pck, SpecR

    Depositor

    Timothy Lu

    Article

    Cleto et al ACS Synth Biol. 2016 Feb 16.

    Insert

    sgRNA pck (NT)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    ptac

    Available Since

    April 12, 2016

    Availability

    Academic Institutions and Nonprofits only

14. C332

    Plasmid

    #141124

    Purpose

    CinI expressing plasmid

    Depositor

    Matthew Bennett

    Article

    Alnahhas et al Nat Commun. 2020 Jul 21;11(1):3659. doi:

    Insert

    CinI (cinI )
    

    Tags

    ssrA degradation tag

    Expression

    Bacterial

    Promoter

    engineered lac promoter

    Available Since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Rec-PhoA/CsgA

    Plasmid

    #170787

    Purpose

    Recombinase controlled expression of csgA and phoA. Expression is activated upon flipping of inverted proD with corresponding recombinase

    Depositor

    Urartu Seker

    Article

    Ahan et al Chembiochem. 2019 Jul 15;20(14):1799-180

    Inserts

    csgA (csgA )
    phoA (phoA )
    

    Use

    Synthetic Biology

    Tags

    HHHHHH

    Expression

    Bacterial

    Promoter

    Inverted proD with Bxb1recognition sites and Inve…

    Available Since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDEST17-ALDH1A3

    Plasmid

    #189750

    Purpose

    The construct was used to express recombinant ALDH1A3 in E.Coli. The purified protein was used for activity assay.

    Depositor

    Robert Sobol

    Article

    Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

    Insert

    ALDH1A3 (ALDH1A3 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28-his6-HA3-SNAPf

    Plasmid

    #188941

    Purpose

    T7 expression vector for making his6- and HA3-tagged SNAP protein

    Depositor

    Stephen Buratowski

    Article

    Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

    Insert

    his6-HA3-SNAPf
    

    Expression

    Bacterial

    Available Since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. RALA

    Plasmid

    #55657

    Purpose

    expression of mouse RALA

    Depositor

    Julian Downward

    Article

    Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

    Insert

    RALA (Rala Mouse)
    

    Tags

    GST and Thrombin cleavage site

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pZ8-P_dCas9

    Plasmid

    #74063

    Purpose

    pZ8-1 plasmid carrying dcas9 driven by the propionate-inducible prpD2 promoter (PprpD2), KanR

    Depositor

    Timothy Lu

    Article

    Cleto et al ACS Synth Biol. 2016 Feb 16.

    Insert

    dcas9
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    Propionate inducible promoter (prp)

    Available Since

    April 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHLU

    Plasmid

    #64167

    Purpose

    harbours marker genes HIS3, LEU2 and URA3

    Depositor

    Markus Ralser

    Article

    Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

    Type

    Empty backbone

    Expression

    Bacterial and Yeast

    Available Since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge