32,115 results
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Plasmid#112561PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and Bla (beta-lactamase from the halophile Chromohalobacter sp. fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneTags8xHis-Bla and Strep tagExpressionBacterialAvailable SinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only
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G3BP1A
Plasmid#42480PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertG3BP1A
TagsHis6-TEVExpressionBacterialMutationSee CommentsAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEMABC
Plasmid#45398DepositorExpressionBacterialPromoterNone and T7Available SinceJune 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBbB6c-GFP
Plasmid#35356DepositorInsertGFP
UseSynthetic BiologyExpressionBacterialPromoterLlacO-1Available SinceFeb. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAOJ15
Plasmid#91567PurposeBroad host range allelic exchange suicide vector for bacteria - Tse2 toxin driven by anhydrotetracycline inducible promoterDepositorTypeEmpty backboneExpressionBacterialPromoterTetracycline promoterAvailable SinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGST2-TSG101 (2-145)
Plasmid#184810PurposeBacterial expression of TSG101 UEV domain (aa 2-145)DepositorAvailable SinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCPP5154
Plasmid#128704PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInsertavrPto
ExpressionBacterialAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSA2
Plasmid#52262PurposeBacterial expression of human 6His-tagged SUMO2 and SUMO-E1DepositorTags6His-tagExpressionBacterialMutationC-terminal gly-glyPromoterT7 and rbsAvailable SinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSMT3-FBF2
Plasmid#166972PurposeExpresses FBF-2 in E. coli for purification and crystallization studiesDepositorInsertFBF-2 (fbf-2 Nematode)
TagsHis-SUMOExpressionBacterialMutationFBF-2 PUM RNA binding domain; amino acids 164-575PromoterT7Available SinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
p007ampGFP
Plasmid#58535PurposeGFP from jellyfish Aquorea sp. under T5 promoter with lac operators; ampicillin resistant.DepositorInsertGreen fluorescent protein
ExpressionBacterialPromoterT5 between two lac operatorsAvailable SinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRG/III-hs-MBP
Plasmid#121676PurposeBacterial expression of MBP fusion proteinsDepositorInsertmaltose binding protein
ExpressionBacterialPromoterlac promoterAvailable SinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZa-tetO-csgB-CmR
Plasmid#89580PurposeExpress csgB curli proteinDepositorInsertcsgB
ExpressionBacterialAvailable SinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1-p19-T7
Plasmid#46306DepositorInsertsp19
DNA-1 site
linker
DNA-2 site
UsePro-sirnaTags6XHis and GSTExpressionBacterialPromoterTacAvailable SinceJuly 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
CAMK2D
Plasmid#38904PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMesh1
Plasmid#175594PurposeLac inducible, AMP Resistant, non labelled gene mesh1 from D. melanogaster on a low copy backbone. Used to induce controllable hydrolysis of ppGpp in E. coli.DepositorInsertMesh1 (Mesh1 Fly)
ExpressionBacterialMutationCodon optimized for translation in E.coli. AGGAGG…Available SinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC57-HBB-nLuc
Plasmid#175432PurposeUsed to generate IVT RNA for translation extractsDepositorInsertHBB-nLuc
UseLuciferaseExpressionBacterialPromoterT7Available SinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET24a MDH_AQUAR
Plasmid#204239PurposeBacterial expression of A. arcticum malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).InsertMDH_AQUAR
Tags6X HisExpressionBacterialPromoterLacAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)-NADPH-LUCID-3 (C50 1.4 uM)
Plasmid#117910PurposePlasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 1.4 uMDepositorInsertSNAP_Pro30_[cpNLuc23_24]eDHFR(R44A)
TagsHis-tag and Strep-tagExpressionBacterialMutationR44A on eDHFRPromoterT7Available SinceOct. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBbE7k-RFP
Plasmid#35315DepositorInsertRFP
UseSynthetic BiologyExpressionBacterialPromoterT7Available SinceFeb. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pKFSS1_2
Plasmid#118227PurposeEmpty E. coli - B. burgdorferi shuttle vector; streptomycin resistantDepositorTypeEmpty backboneExpressionBacterialAvailable SinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only