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34,238 results

Showing: 7721 - 7740 of 34238 results

  1. rpoDs366C

    Plasmid
    #129688
    Purpose
    Expression of Sigma 70 S366C
    Depositor
    Jeff Gelles
    Publication
    Mumm et al bioRxiv 2020
    Insert
    rpoD (rpoD E. coli)
    Tags
    His6/thrombin
    Expression
    Bacterial
    Mutation
    C132S C291S C295S S366C
    Available since
    May 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    129688-plasmid-map-sequence-id-270569

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  3. pEVOL-AcKRS-CloDF

    Plasmid
    #127415
    Purpose
    Mutant of mmPylRS designed for incorporation of non-canonical amino acids (acyl-lysine derivatives) in to M13 bacteriophage
    Depositor
    Wenshe Liu
    Publication
    Wang et al Angew Chem Int Ed Engl. 2019 Oct 28;58(4
    Insert
    Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
    Expression
    Bacterial
    Promoter
    araBAD
    Available since
    April 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    127415-plasmid-map-sequence-id-250212

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  4. pAcHELIX-sgRNA_entry (CJT94)

    Plasmid
    #181783
    Purpose
    Expresses AcHELIX containing a nicking I-AniI fusion to AcTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Publication
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-AcTnsB, AcTnsC, AcTniQ, AcCas12k
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    181783-plasmid-map-sequence-id-358223

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  9. pSL-1

    Plasmid
    #180422
    Purpose
    Kan resistant plasmid derived from pBTK402, a broad-host-range plasmid previously shown to replicate in multiple species of bee gut bacteria. It encodes a low level RFP promoter.
    Depositor
    Nancy Moran
    Publication
    Li et al Proc Natl Acad Sci U S A. 2022 May 3;119
    Insert
    Red Chromprotein gene
    Expression
    Bacterial
    Available since
    March 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    180422-plasmid-map-sequence-id-357215

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  11. pET-NlaCascade-NLS-6xHis

    Plasmid
    #180213
    Purpose
    Expressing Neisseria lactamica Type I-C Cascade subunits (Cas5-Cas8-Cas11-Cas7) with NLS and His tag on the C terminus of Cas7 for protein purification in E.Coli
    Depositor
    Yan Zhang
    Publication
    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.
    Insert
    NlaCas5-Cas8-Cas7
    Tags
    6XHis and NLS
    Expression
    Bacterial
    Promoter
    T7
    Available since
    March 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    180213-plasmid-map-sequence-id-356902

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  13. pNLS-2xBFP(4C)

    Plasmid
    #176151
    Purpose
    Encodes NLS-2xBFP(4C), which is a tandem BFP with an N-terminal SV40 NLS. The protein has 4 solvent accessible cysteines for labeling with maleimide dyes.
    Depositor
    Siegfried Musser
    Publication
    Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d
    Insert
    NLS-2xBFP(4C)
    Tags
    6xHis tag and SV40 nuclear localization sequence …
    Expression
    Bacterial
    Mutation
    Compared with EGFP, the two BFP domains have the …
    Promoter
    Ptrc
    Available since
    March 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    176151-plasmid-map-sequence-id-356068

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  14. pShHELIX(Cas12k-TniQ-TniQ)-sgRNA_entry (CJT112)

    Plasmid
    #181791
    Purpose
    Expresses 3-component ShHELIX containing a Cas12k-TniQ-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Publication
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-ShTnsB, ShTnsC, ShCas12k-ShTniQ-ShTniQ
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    181791-plasmid-map-sequence-id-354814

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  15. PRDX1A-c001

    Plasmid
    #175473
    Purpose
    Protein expression in bacterial cells. Entire CDS, M1-K199. Can be biotinylated.
    Depositor
    Opher Gileadi
    Insert
    PRDX1 (PRDX1 Human)
    Tags
    AviTag and His6-TEV
    Expression
    Bacterial
    Available since
    Jan. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    175473-plasmid-map-sequence-id-353180

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  16. EPB41L3A-c000

    Plasmid
    #175458
    Purpose
    Protein expression in bacterial cells. Entire CDS, M1-D1087.
    Depositor
    Opher Gileadi
    Insert
    EPB41L3 (EPB41L3 Human)
    Tags
    His6-TEV
    Expression
    Bacterial
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175458-plasmid-map-sequence-id-353141

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  17. pMTS-hFluc

    Plasmid
    #177716
    Purpose
    Firefly luciferase reporter fused to a mitochondria signal sequence for mitochondrial targeting
    Depositor
    Erik Böttger
    Publication
    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hFluc
    Tags
    Mitochondrial Targeting Sequence MTS SS
    Expression
    Bacterial, Mammalian, and Y…
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    177716-plasmid-map-sequence-id-352607

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  18. pATZ-BFP-HA-GFP11

    Plasmid
    #177721
    Purpose
    ER protein alpha-1-antitrypsin containing E342K mutation (ATZ mutant) fused to blue fluorescence protein (BFP) tag protein, an HA tag and the eleventh β-strand of GFP
    Depositor
    Erik Böttger
    Publication
    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    alpha-1-antitrypsin (SERPINA1 Human)
    Tags
    GFP11, HA, and TagBFP
    Expression
    Bacterial and Mammalian
    Mutation
    E342K (ATZ mutant)
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    177721-plasmid-map-sequence-id-352621

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Showing: 7721 - 7740 of 34238 results