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33,459 results

Showing: 7721 - 7740 of 33459 results

  4. pET28a(+)::MBP-NL3F10H

    Plasmid
    #141291
    Purpose
    MBP:NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)
    Depositor
    Andrew Millar
    Publication
    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10
    Insert
    MBP-NLUC3F10H
    Use
    Luciferase
    Tags
    Flag (x3), His (x10), and MBP
    Expression
    Bacterial
    Mutation
    Engineered for high stability (t1/2 = 11.5 days a…
    Promoter
    promoter for bacteriophage T7 RNA polymerase
    Available since
    Feb. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    141291-plasmid-map-sequence-id-324616

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  9. p1A

    Plasmid
    #162692
    Purpose
    Expresses H. neapolitanus CbbLS and S. elongatus Prk. Rescues CCMB1 in M9 glycerol at 10% CO2
    Depositor
    David Savage
    Publication
    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1
    Insert
    H. neapolitanus CbbLS and S. elongatus Prk
    Tags
    N terminal 6x His tag on PRK
    Expression
    Bacterial
    Promoter
    PLtet0-1 promoter
    Available since
    Feb. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    162692-plasmid-map-sequence-id-324151

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  10. pExp-TF

    Plasmid
    #112575
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and TF (Trigger factor (tig) from E. coli) fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8xHis-TriggerFactor and Strep tag
    Expression
    Bacterial
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112575-plasmid-map-sequence-id-217211

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  11. pExp-mCherry

    Plasmid
    #112569
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and mCherry (mCherry fluorescent protein,) fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8xHis, Strep II, and mCherry
    Expression
    Bacterial
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112569-plasmid-map-sequence-id-217213

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  14. pDest-557

    Plasmid
    #161890
    Purpose
    Standard Gateway (attR1-attR2) vector for expression of maltose-binding protein (MBP) fusions in E. coli
    Depositor
    Dominic Esposito
    Type
    Empty backbone
    Tags
    MBP
    Expression
    Bacterial
    Available since
    Jan. 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    161890-plasmid-map-sequence-id-322136

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  16. katushka-FMDV-P2A-pTRIP

    Plasmid
    #163522
    Purpose
    Used to construct lentivirus to express katushka in mammalian cells with option for a second insert
    Depositor
    Thomas Weber
    Insert
    stopless Katushka-P2A
    Use
    Lentiviral
    Expression
    Bacterial and Mammalian
    Available since
    Jan. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    163522-plasmid-map-sequence-id-321575

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  18. pCJ207

    Plasmid
    #162680
    Purpose
    pET-21b(+) based plasmid for expression of the putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with C-terminal His tag, codon optimized for expression in E. coli K12.
    Depositor
    Gregg Beckham
    Publication
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    Putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with signal peptide
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    162680-plasmid-map-sequence-id-321455

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  19. pCJ208

    Plasmid
    #162681
    Purpose
    Lidded variant of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) removing a seven-residue loop of PETase (Trp185:Phe191, PETase numbering) and replacing it with Gly251:Thr472 from MHETase; codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Publication
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) incorporating the MHETase lid
    Tags
    His
    Expression
    Bacterial
    Mutation
    Replacement of W185-F191 with G251-T472 from MHET…
    Promoter
    T7/lac
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    162681-plasmid-map-sequence-id-321444

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  20. pCJ209

    Plasmid
    #162682
    Purpose
    Lidless variant of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) replacing Gly251:Thr472 of MHETase with the 7-residue loop (Trp185:Phe191, PETase numbering) of PETase , codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Publication
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) with the lid removed
    Tags
    His
    Expression
    Bacterial
    Mutation
    Replacement of G251-T472 with W185-F191 from PETa…
    Promoter
    T7/lac
    Available since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    162682-plasmid-map-sequence-id-320836

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Showing: 7721 - 7740 of 33459 results