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PZac2.1 hSyn1-HA-BioID2-SAPAP3

Plasmid

#176857

Purpose

Expresses SAPAP3 protein fused with BioID2 at the N-terminus in neurons

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

HA-BioID2-SAPAP3

Use

AAV

Tags

HA

Expression

Bacterial

Promoter

hSyn1

Available Since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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pTA-attB

Plasmid

#18937

Depositor

Michele Calos

Article

Groth et al Proc Natl Acad Sci U S A. 2000 May 23. 9

Insert

phiC31 attB

Expression

Bacterial

Available Since

Oct. 17, 2008

Availability

Academic Institutions and Nonprofits only

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pFPV25.1_rpsM_mCerulean3

Plasmid

#124904

Purpose

rpsM promoter (S. typhimurium) driving mCerulean3 (codon-optimized for S. typhimurium)

Depositor

Markus Covert

Article

Lane et al Cell Syst. 2019 Mar 27;8(3):183-196.e4.

Insert

rpsM promoter and mCerulean3

Expression

Bacterial

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pET PPL His6 MBP LIC cloning vector (2K-T)

Plasmid

#37183

Depositor

Scott Gradia

Type

Empty backbone

Tags

His6, MBP, and PPL

Expression

Bacterial

Promoter

T7

Available Since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pAJM.969

Plasmid

#108537

Purpose

MphRAM + EryR + PMph-YFP Reporter. Ery sensor for use in non-Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

MphRAM + EryR + PMph-YFP

Expression

Bacterial

Mutation

MphR: N94D

Available Since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)-cpHalo∆

Plasmid

#194659

Purpose

Protein production of the split HaloTag part cpHalo∆ (cpHaloTag135/156) in bacteria

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

cpHalo∆

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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SND1 (SND1A-c003)

Plasmid

#98242

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

SND1A (SND1 Human)

Tags

His-6-TEV

Expression

Bacterial

Mutation

pfam00567:TUDOR 677-799

Promoter

T7

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pHLU

Plasmid

#64167

Purpose

harbours marker genes HIS3, LEU2 and URA3

Depositor

Markus Ralser

Article

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn5-153

Plasmid

#118535

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

sYFP2

Use

Minitn5 delivery vector

Expression

Bacterial

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pVRa41_491

Plasmid

#49702

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF41_491

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pEB2_mScarletI_mVenusNB-MrkHR113A

Plasmid

#182292

Purpose

expresses c-di-GMP mutant biosensor in E. coli

Depositor

Howard Berg

Article

Vrabioiu et al Proc Natl Acad Sci U S A. 2022 Feb 8;119

Insert

mVenusNB-MrkHR113A

Tags

mVenusNB internal fusion to MrkH with a R113A poi…

Expression

Bacterial

Mutation

changed arginine 113 to alanine, MrkH numbering …

Promoter

proC

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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CFL1 (CFL1A-c002)

Plasmid

#98237

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

CFL1A (CFL1 Human)

Tags

His-6-TEV

Expression

Bacterial

Mutation

pfam00241:Cofilin_ADF 27-148

Promoter

T7

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pKEW212-MBP-TEV-AcrVA4

Plasmid

#115658

Purpose

Expresses AcrVA4 in bacteria via T7 RNAP, for purification

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

AcrVA4

Tags

MBP-TEV

Expression

Bacterial

Promoter

T7 RNAP

Available Since

Oct. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pBSFlp

Plasmid

#45470

Depositor

Michele Swanson

Article

Bryan et al Appl Environ Microbiol. 2011 Apr;77(7):2

Insert

Flp recombinase

Expression

Bacterial

Promoter

T7

Available Since

June 4, 2013

Availability

Academic Institutions and Nonprofits only

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pSL4369 (pEffector_TnpB2,ωRNA)

Plasmid

#210871

Purpose

Expresses GstTnpB2 and native ωRNA from constitutive promoters

Depositor

Samuel H. Sternberg

Article

Meers et al Nature. 2023 Sep 27. doi: 10.1038/s41586

Insert

GstTnpB2

Expression

Bacterial

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pET His6 Mocr TEV LIC cloning vector (2O-T)

Plasmid

#29710

Depositor

Scott Gradia

Type

Empty backbone

Tags

His6-Mocr-TEV

Expression

Bacterial

Available Since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-HA-ERK2 R67S

Plasmid

#53201

Purpose

Expresses GST and HA tagged human ERK2 R67S from bacterial expression vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

ERK2 (MAPK1 Human)

Tags

GST and HA

Expression

Bacterial

Mutation

R67S

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only
pTpal-fdc

Plasmid

#78288

Purpose

Expresses A. thaliana PAL2 and S. cerevisiae FDC1 in E. coli

Depositor

David Nielsen

Article

McKenna et al Biotechnol J. 2013 Dec;8(12):1465-75. do

Inserts

PAL2 (PAL2 Mustard Weed)
FDC1 (FDC1 Budding Yeast)

Expression

Bacterial

Mutation

*see below

Promoter

trc

Available Since

June 1, 2016

Availability

Academic Institutions and Nonprofits only

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pQLinkHD_ECFP

Plasmid

#118857

Purpose

fluorescent protein expression with an N-terminal His-tag

Depositor

Marc Birtwistle

Article

Holzapfel et al ACS Comb Sci. 2018 Nov 12;20(11):653-659

Insert

ECFP

Tags

His-tag

Expression

Bacterial

Available Since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pDx_mRuby3-SYFP2

Plasmid

#189758

Purpose

Dual expression vector for bacteria and mammalian cells producing mRuby3-SYFP2 fusion protein for FRET analysis

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mRuby3-SYFP2

Tags

6xHis

Expression

Bacterial and Mammalian

Promoter

CMV & Rha

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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