30,180 results

1. pUdO4k

   Plasmid

   #210237

   Purpose

   low-copy plasmid for bacterial expression

   Depositor

   François-Xavier Campbell-Valois

   Article

   Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pUdO2c

   Plasmid

   #210231

   Purpose

   medium-copy plasmid for bacterial expression

   Depositor

   François-Xavier Campbell-Valois

   Article

   Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGEX-2T-Rac1-T17N

   Plasmid

   #12978

   Depositor

   Gary Bokoch

   Insert

   Rac1 DN (RAC1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   T17N Dominant negative

   Promoter

   Available since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

4. SNTB2

   Plasmid

   #38912

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   SNTB2 (SNTB2 Human)
   

   Use

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pKD077

   Plasmid

   #136473

   Purpose

   SSB-mTur2 IDL fusion expression plasmid

   Depositor

   James Keck

   Article

   Dubiel et al Nucleic Acids Res. 2020 Jun 19;48(11):60

   Insert

   SSB-mTur2 (ssb E. coli)
   

   Use

   Tags

   mTurquoise2

   Expression

   Bacterial

   Mutation

   mTurquoise2 inserted between Phe148 and Ser149

   Promoter

   T7 promoter

   Available since

   April 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. TPH2

   Plasmid

   #73243

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   TPH2 (TPH2 Human)
   

   Use

   Tags

   His-6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pNAS1B split mCherry N-ME(pS)VD/TRAP(RK)-C

   Plasmid

   #61966

   Purpose

   Positive control duet plasmid for SepOTS-dependent split mCherry assembly assay (i.e., N-mCherry is fused to the peptide ME(pS)VD (pS = phosphoserine); C-mCherry is fused to TRAP(RK))

   Depositor

   Lynne Regan

   Article

   Sawyer et al ACS Chem Biol. 2014 Nov 21;9(11):2502-7.

   Inserts

   N-mCherry-ME(pS)VD
   TRAP(RK)-C-mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PBAD and PLtetO

   Available since

   Feb. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSC5GGv2

   Plasmid

   #188604

   Purpose

   pSC5 Golden-Gate Cloning Plasmid (BsaI - Site 2)

   Depositor

   Bogumil Karas

   Article

   Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

   Type

   Empty backbone

   Use

   Synthetic Biology; Diatom expression

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. 4IJ8

   Plasmid

   #51327

   Depositor

   Cheryl Arrowsmith

   Insert

   4IJ8 (KMT5A Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Truncation: See Comments

   Promoter

   T7

   Available since

   Feb. 12, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

10. Human _TFAM_NoMTS_Dimer_pET28a+

    Plasmid

    #60013

    Purpose

    Expresses TFAM No MTS dimerization mutant in bacterial cells

    Depositor

    David Chan

    Article

    Ngo et al Nat Commun. 2014;5:3077. doi: 10.1038/nc

    Insert

    Human TFAM No MTS (TFAM Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Missing 1st 42 aas (Mitochondrial targeting seque…

    Promoter

    T7

    Available since

    Oct. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. JMJD1CA-c089

    Plasmid

    #79644

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    JMJD1C (JMJD1C Human)
    

    Use

    Tags

    6x His and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pIntegrase_5

    Plasmid

    #60577

    Purpose

    plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Integrase_5
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pBAD

    Available since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. HSPA1LA

    Plasmid

    #42429

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    HSPA1LA (HSPA1L Human)
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    See Comments

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pMYO127TAG-SepT

    Plasmid

    #34625

    Depositor

    Jesse Rinehart, Dieter Söll

    Article

    Park et al Science. 2011 Aug 26;333(6046):1151-4.

    Insert

    Myoglobin-127TAG
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    codon-optimized + an amber stop codon was introdu…

    Promoter

    Ipp

    Available since

    Feb. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a-C9ORF80

    Plasmid

    #128418

    Purpose

    Express C9ORF80 in E. coli with a His tag (C-terminal)

    Depositor

    Yuliang Wu

    Article

    Vidhyasagar et al Biochem J. 2018 Jan 2;475(1):45-60. doi:

    Insert

    C9ORF80 (INIP Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CL7-LbCas12aD156R

    Plasmid

    #164659

    Purpose

    Bacterial expression of mutated LbCas12aD156R using CL7/Im7

    Depositor

    Piyush Jain

    Article

    Nguyen et al Methods. 2021 Feb 9. pii: S1046-2023(21)

    Insert

    LbCas12a-D156R
    

    Use

    Tags

    Thioredoxin, CL7, SUMO

    Expression

    Bacterial

    Mutation

    changed Aspartic Acid 156 to Arginine

    Promoter

    T7

    Available since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pOG231

    Plasmid

    #17736

    Depositor

    Geoff Wahl

    Article

    O'Gorman et al Proc Natl Acad Sci U S A. 1997 Dec 23;94

    Insert

    NLS-Cre recombinase
    

    Use

    Cre/Lox

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 14, 2008

    Availability

    Academic Institutions and Nonprofits only

18. pMPMA3dPlac-PssaG-GFPLVA

    Plasmid

    #23343

    Depositor

    Olivia Steele-Mortimer

    Article

    Ibarra et al Microbiology (Reading). 2010 Apr;156(Pt

    Insert

    GFP(LVA)
    

    Use

    Tags

    GFP(LVA)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 27, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pBSSK p85 alpha (delta iSH2)

    Plasmid

    #13432

    Depositor

    Ronald Kahn

    Article

    Taniguchi et al Proc Natl Acad Sci U S A. 2006 Aug 8. 10

    Insert

    p85 alpha (delta iSH2) (PIK3R1 Human)
    

    Use

    Tags

    flag

    Expression

    Bacterial

    Mutation

    Dominant negative p85 alpha. The p110 alpha bindi…

    Promoter

    Available since

    Nov. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

20. pGEX6P2-MDMX (23-111)

    Plasmid

    #62064

    Purpose

    Expression of human MDM4 (residues 23-111) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

    Insert

    MDM4 (MDM4 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    contains residues 23-111

    Promoter

    tac

    Available since

    Feb. 5, 2015

    Availability

    Academic Institutions and Nonprofits only