30,424 results

1. pNatA (pACYCduet-naa10-naa15)

   Plasmid

   #72928

   Purpose

   Allows expression of a modified version of the fission yeast NatA complex - chloramphenicol marker

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187

   Inserts

   naa10 (naa10 Synthetic, Fission Yeast)
   naa15 (naa15 Fission Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Codon optomised for E.coli expression

   Promoter

   T7

   Available Since

   Feb. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHyperTEV60

   Plasmid

   #228496

   Purpose

   10xHis-SUMO (Smt3) tagged HyperTEV60 engineered protease (E. coli based protein expression)

   Depositor

   Richard Baker

   Insert

   TEV Protease
   

   Use

   Tags

   10xHis-SUMO(Smt3)

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available Since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBDC

   Plasmid

   #135188

   Purpose

   Integration and excision of chloromaphenicol cassette for the E. coli genome via CreI, SceI, and lamba Red system. Contains SacB selection.

   Depositor

   Hongqing Fang

   Article

   Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d

   Insert

   CmR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   cat

   Available Since

   March 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Tn7 helper plasmid

   Plasmid

   #141161

   Purpose

   Temperature-sensitive plasmid carries tnsABCD genes that encode transposase biochemical machinery under an arabinose-inducible pBAD promoter

   Depositor

   Shimon Bershtein

   Article

   Jasinska et al Nat Ecol Evol. 2020 Feb 24. pii: 10.1038

   Insert

   Tn7 transposon machinery
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Arabinose-inducible pBAD

   Available Since

   Aug. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSNA

   Plasmid

   #135192

   Purpose

   Arabinose inducible expression of lamba-Red recombinase proteins and I-SceI. Contains ampicillin resistance cassette

   Depositor

   Hongqing Fang

   Article

   Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d

   Insert

   I-SceI lamba Red
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   araBAD

   Available Since

   May 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Raf-1 GST RBD 1-149

   Plasmid

   #13338

   Depositor

   Channing Der

   Article

   Brtva et al J Biol Chem. 1995 Apr 28. 270(17):9809-1

   Insert

   Raf-1 (RAF1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 20, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pOSIP-CT (CamR, P21)

   Plasmid

   #45981

   Depositor

   Keith Shearwin, Drew Endy

   Article

   St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pT7II-2N4Rtau

   Plasmid

   #177653

   Purpose

   Recombinant protein synthesis of human Tau

   Depositor

   Jeff Kuret

   Article

   Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

   Insert

   Tau (2N4R) (MAPT Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCAG-BirA*-dCas9-eGFP

   Plasmid

   #84779

   Purpose

   Expresses BirA*-dCas9-eGFP in mammalian cells.

   Depositor

   Heinrich Leonhardt

   Article

   Schmidtmann et al Nucleus. 2016 Sep 27:0.

   Insert

   BirA*-dCas9-eGFP
   

   Use

   CRISPR

   Tags

   cMyc

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   July 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pATetO 6XHis T4 DNA Ligase

    Plasmid

    #206207

    Purpose

    Inducible expression of T4 DNA ligase

    Depositor

    Andrew Ellington

    Article

    Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

    Insert

    T4 DNA ligase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge