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33,526 results

Showing: 7981 - 8000 of 33526 results

  1. pDB036a

    Plasmid
    #99215
    Purpose
    Accessory plasmid for PACE of MjTyrRS variants; negative selection
    Depositor
    David Liu
    Publication
    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038
    Insert
    Ppsp [SD8] gIII; PproK tyrT(Opt,CUA); PproD [SD4] T7RNAP(S12*,S203*)
    Expression
    Bacterial
    Mutation
    amber codons at positions 12 and 203 of T7 RNA po…
    Promoter
    Ppsp, PproK and PproD
    Available since
    Oct. 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    99215-plasmid-map-sequence-id-192376

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  4. Keasling-2337

    Plasmid
    #100168
    Purpose
    Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth
    Depositor
    Jay Keasling
    Publication
    Anthony et al Metab Eng. 2009 Jan;11(1):13-9. doi: 10.
    Insert
    lacIq-Ptrc-ADS
    Expression
    Bacterial
    Promoter
    trc
    Available since
    Sept. 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    100168-plasmid-map-sequence-id-193496

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  5. Keasling-2187

    Plasmid
    #100167
    Purpose
    Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth
    Depositor
    Jay Keasling
    Publication
    Anthony et al Metab Eng. 2009 Jan;11(1):13-9. doi: 10.
    Insert
    MevT and MBIS operon
    Expression
    Bacterial
    Promoter
    lacUV5
    Available since
    Sept. 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    100167-plasmid-map-sequence-id-193500

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  6. TRIM33 (TRIM33B-c100)

    Plasmid
    #98243
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    TRIM33B (TRIM33 Human)
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    pfam00628:PHD 889-932, pfam00439:Bromodomain 963-…
    Promoter
    T7
    Available since
    Sept. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98243-plasmid-map-sequence-id-193421

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  7. CFL1 (CFL1A-c002)

    Plasmid
    #98237
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    CFL1A (CFL1 Human)
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    pfam00241:Cofilin_ADF 27-148
    Promoter
    T7
    Available since
    Sept. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98237-plasmid-map-sequence-id-193416

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  13. pEHIShqprt

    Plasmid
    #92166
    Purpose
    Plasmid pEHIShqprt expressing the full-length of human quinolinate phosphoribosyltransferase (hQPRTase) was constrcucted by inserting BspHI/BamHI-fragment into NcoI/BamHI digested pEHISTEV vector
    Depositor
    Huanting Liu, James Naismith
    Publication
    Liu et al J Mol Biol. 2007 Oct 26;373(3):755-63. E
    Insert
    Quinolinate Phosphoribosyltransferase (QPRT Human)
    Tags
    TEV protease -cleavable N-terminal 6His
    Expression
    Bacterial
    Mutation
    changed Asp 2 to Asn to create BspHI site
    Promoter
    T7
    Available since
    Aug. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    92166-plasmid-map-sequence-id-190226

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  20. pBAD mApple

    Plasmid
    #91772
    Purpose
    Bacterial expression of a fluorescent protein, monomeric Apple (mApple). Note: Addgene identified several mutations compared to the final published sequence.
    Depositor
    Erik Rodriguez, Roger Tsien
    Publication
    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:
    Insert
    monomeric Apple
    Tags
    Hexa-Histidine tag, Xpress epitope for detection …
    Expression
    Bacterial
    Mutation
    R97K, E152S, A180S, V207T compared to mApple (FP …
    Promoter
    araBAD
    Available since
    June 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    91772-plasmid-map-sequence-id-181643

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Showing: 7981 - 8000 of 33526 results