30,875 results

1. pBYK01

   Plasmid

   #138656

   Purpose

   phiOZJ integrating Streptomyces expression vector lacking RDF

   Depositor

   Joshua Blodgett

   Article

   Ko et al J Ind Microbiol Biotechnol. 2019 Nov 8.

   Type

   Empty backbone

   Expression

   Bacterial

   Promoter

   PermE* variant sequence from pDA1652

   Available Since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. cryS96

   Plasmid

   #67519

   Purpose

   αB-crystallin minipeptide fused to ELP (S96).

   Depositor

   Andrew MacKay

   Article

   Wang et al J Control Release. 2014 Oct 10;191:4-14.

   Insert

   mini-αB-crystallin fused to ELP S96 (VPGSG) (CRYAB Human, Synthetic)
   

   Tags

   elastin-like polypeptide (ELP) S96

   Expression

   Bacterial

   Mutation

   mini-αB-crystallin contains residues 73–92 of aB-…

   Promoter

   T7

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pOP/SSAT

   Plasmid

   #140291

   Purpose

   Expresses human SSAT in bacterial cells

   Depositor

   Saverio Bettuzzi, Gaetano Marverti

   Article

   Marverti et al Carcinogenesis. 2005 Oct;26(10):1677-86.

   Insert

   SSAT1 (SAT1 Human)
   

   Expression

   Bacterial

   Available Since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET21c(+)-EFG1mt

   Plasmid

   #31076

   Depositor

   Linda Spremulli

   Article

   Bhargava et al Protein Expr Purif. 2004 Oct . 37(2):368

   Insert

   Mature form human mitochondrial elongation factor G isoform 1 (GFM1 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Includes the coding sequence for the mature form.…

   Available Since

   Sept. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

5. ETV1_pet28a

   Plasmid

   #131644

   Purpose

   Bacterial expression of ETS gene ETV1

   Depositor

   Peter Hollenhorst

   Article

   Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

   Insert

   ETV1 (ETV1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMC051-HisMS2_PLP_pac_LC

   Plasmid

   #128234

   Purpose

   Lower Copy expression construct for MS2 phage like particles with Esp3I cloning sites for packaged RNA.

   Depositor

   Paul Freemont

   Article

   de Martin Garrido et al Mol Microbiol. 2019 Oct 16. doi: 10.1111

   Inserts

   Maturation Protein
   Coat Protein Dimer
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 15, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pFREE2-SpCas9-sgRNA

   Plasmid

   #179582

   Purpose

   Expresses spCas9 and gRNA that targets ColE1 plasmids for curing

   Depositor

   Kevin Solomon

   Article

   Lee et al ACS Synth Biol. 2022 Jan 21;11(1):53-60.

   Insert

   Cas9
   

   Expression

   Bacterial

   Available Since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET27b-ecDHFR-N23PP/L28F

   Plasmid

   #118584

   Purpose

   Bacterial expression of E.coli DHFR N23PP and L28F mutant

   Depositor

   Stephen Benkovic

   Article

   Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

   Insert

   DHFR
   

   Expression

   Bacterial

   Mutation

   L28 mutated to F28, N23 mutated to P23, a proline…

   Promoter

   T7

   Available Since

   Nov. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX-mdm2/2702

   Plasmid

   #11492

   Depositor

   Allan Weissman

   Article

   Fang et al J Biol Chem. 2000 Mar 24. 275(12):8945-5

   Insert

   mdm2 (MDM2 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   n/a

   Available Since

   April 20, 2006

   Availability

   Academic Institutions and Nonprofits only

10. pRoRH

    Plasmid

    #213133

    Purpose

    Riboptoregulator circuit RoRH for blue-light-activated gene expression

    Depositor

    Andreas Moeglich

    Article

    Ranzani et al Nucleic Acids Res. 2024 Aug 10:gkae678.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-45b-HOXD13(+7A)-IDR-mCherry

    Plasmid

    #145281

    Purpose

    Bacterial Expression of 6xHis HOXD13 (+7A) IDR mCherry Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXD13(+7A)-IDR (HOXD13 Human)
    

    Expression

    Bacterial

    Mutation

    A-repeat expanded

    Available Since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. NEK1_HUMAN_D0

    Plasmid

    #79725

    Purpose

    This plasmid encodes the kinase domain of NEK1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    NEK1 (NEK1 Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

13. 4IJ8

    Plasmid

    #51327

    Depositor

    Cheryl Arrowsmith

    Insert

    4IJ8 (KMT5A Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Truncation: See Comments

    Promoter

    T7

    Available Since

    Feb. 12, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

14. pTXG

    Plasmid

    #52280

    Purpose

    Bacterial expression of TARGET proteins C-terminally fused to GyrA intein and GST

    Depositor

    Primo Schaer

    Article

    Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

    Type

    Empty backbone

    Tags

    GyrA-intein-GST

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. PE_26: Para_Lac_Tet_eG::sfYFP

    Plasmid

    #104887

    Purpose

    PE_26: Para_Lac_Tet_eG::sfYFP

    Depositor

    Matthew Bennett

    Article

    Chen et al Nat Commun. 2018 Jan 4;9(1):64. doi: 10.

    Insert

    Hybrid Promoter Para_Lac_Tet_eG
    

    Expression

    Bacterial

    Promoter

    Para_Lac_Tet_eG

    Available Since

    Jan. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pnEA-vH_His-TEV-SEPT2-sfCherry2_SEPT6

    Plasmid

    #180313

    Purpose

    bacterial co-expression of human SEPT2 fused to superfolder Cherry2 and of human SEPT6

    Depositor

    Manos Mavrakis

    Article

    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

    Inserts

    SEPTIN2 (SEPTIN2 Human)
    SEPTIN6 (SEPTIN6 Human)
    

    Tags

    His6-TEV and sfCherry2

    Expression

    Bacterial

    Available Since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET23-CBD-(-PP)-EGFP

    Plasmid

    #12597

    Depositor

    Klaus Hahn

    Article

    Nalbant et al Science. 2004 Sep 10. 305(5690):1615-9.

    Insert

    Cdc42 Biosensor (WAS Human)
    

    Tags

    6xHis and EGFP

    Expression

    Bacterial

    Mutation

    F271C

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDE43-MCK

    Plasmid

    #49523

    Purpose

    Kanamycin and Chloramphenicol resistant; destination vector engineered to integrate into the mycobacteriophage att-L5 site and designed to be used with the multisite Gateway cloning system

    Depositor

    Dirk Schnappinger

    Article

    Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Jan. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJ23119-sgRNA

    Plasmid

    #113654

    Purpose

    sgRNA targeting unit plasmid. The sgRNA targeting unit plasmids contain connector ConLS, ConR1, and one sgRNA transcriptional unit.

    Depositor

    Jeffrey Barrick

    Article

    Geng et al ACS Synth Biol. 2019 Mar 15;8(3):521-531

    Insert

    promoter J23119 and sgRNA
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMG81-MBP-t-M.MpeI-N374K-His

    Plasmid

    #197985

    Purpose

    Expresses CpG Carboxymethyltransferase (M.MpeI N374K) in bacteria. The gene also encodes an N-terminal (TEV cleavable) MBP tag as well as C-terminal His tag.

    Depositor

    Rahul Kohli

    Article

    Wang et al Nat Chem Biol. 2023 Jun 15. doi: 10.1038

    Insert

    MBP-t-M.MpeI-N374K-His
    

    Tags

    His and MBP

    Expression

    Bacterial

    Mutation

    M.MpeI-N374K

    Available Since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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