33,128 results
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Plasmid#216229PurposeS. aureus USA300 GyrA Codon-optimized for E. coliDepositorInsertDNA gyrase
Tags6xHisExpressionBacterialPromoterT7Available SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-Duet1-His-TEV-mCherry-p62
Plasmid#199780PurposeUsed for the expression of mCherry labelled p62 (SMC Internal No. 391).DepositorAvailable SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCL1324
Plasmid#217965PurposeBacterial expression of Importin-5 tagged on its N-terminus with GST. Cleavable with 3C protease.DepositorAvailable SinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYH78
Plasmid#217386PurposePtrc99A-PAmCherry for bacterial expressionDepositorInsertPAmCherry
ExpressionBacterialAvailable SinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBroad-T7RNAP-apraR
Plasmid#199993PurposeT7 RNAP circuit variant T15 on ultra broad host range vector (RSF1010 and pAMB1 origins) apraADepositorInsertT7RNAP Circuit
ExpressionBacterialMutationcopFnullAvailable SinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACRISPR-TP
Plasmid#203589PurposeA sgRNA expression plasmid for genome editing in BurkholderiaDepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFN18A-HaloTag-SpyCatcher
Plasmid#206041PurposeHaloTag-Spy0128-(Protein of Interest)-Spy0128-SpyCatcher-8xHisTagDepositorTypeEmpty backboneTagsHaloTag and SpyCatcher-8HisExpressionBacterialPromoterT7Available SinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-FL-syt1
Plasmid#213628PurposeBacterial expression of SUMO-tagged rat FL-syt1DepositorAvailable SinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGR618
Plasmid#213786PurposeIntegrates at the CAN1 locus the BB-Tv TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassetteDepositorInsertBB-Tv TP-DNAP1
UseSynthetic BiologyExpressionBacterial and YeastAvailable SinceMarch 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)-cpHalo∆
Plasmid#194659PurposeProtein production of the split HaloTag part cpHalo∆ (cpHaloTag135/156) in bacteriaDepositorInsertcpHalo∆
TagsHis-tagExpressionBacterialPromoterT7Available SinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKERepPar
Plasmid#211827PurposeMedium copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein and a RP4 partitioning system to increase segregational stability.DepositorInserteGFP
ExpressionBacterialPromoterT5Available SinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET23a-H6-TEV-cSrc
Plasmid#214233PurposeBacterial expression of the kinase domain of cSrc with a TEV-cleavable N-terminal 6xHis affinity tag; For in vitro tyrosine phosphorylation in peptides/proteins and for kinase specificity screeningsDepositorAvailable SinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKESaPar
Plasmid#211829PurposeLow copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein and a RP4 partitioning system to increase segregational stability.DepositorInserteGFP
ExpressionBacterialPromoterT5Available SinceFeb. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
Skp
Plasmid#213139PurposeExpresses chaperone protein SKPDepositorAvailable SinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCYP64
Plasmid#212897PurposeExpresses the Priestia megaterium LRD-modifying cytochrome P450 enzymeDepositorInsertcytochrome P450
ExpressionBacterialMutationP450BM3 MERO1 (V78A, A82G, F87V, P142S, T175I, A1…PromoterPcymRCAvailable SinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD085
Plasmid#212922PurposeEncodes the HA-tagged 649 Stalk yeast surface display anchor protein as a Type 4a part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsert649 Stalk
ExpressionBacterialAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEK39
Plasmid#210599PurposeReporter gene expression in B. dendrobatidisDepositorInsertSpH2Bpro-Hshph-G4S-HsmRuby3-ScADH1ter
TagsmRuby3ExpressionBacterial and YeastPromoterSpH2BproAvailable SinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pINTO_7/8
Plasmid#206431PurposeA vector backbone (pCC1BAC-based) containing a single SapI recognition site. When digested, it exposes overhangs that allow integration of the vector into the P. tricornutum chloroplast genome.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC19-link-sfGFP-FLAG:Kan
Plasmid#211833PurposePlasmid template that encodes the fluorescent protein sfGFP with a FLAG tag next to kanamycin resistance gene. This template was used to copy out sfGFP-FLAG-Kan for genetic tagging.DepositorInsertsfGFP-FLAG-Kan
ExpressionBacterialMutationNAAvailable SinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLP1
Plasmid#209988PurposeEntry vector to store Level 0 partsDepositorTypeEmpty backboneExpressionBacterialAvailable SinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only