32,127 results
-
Plasmid#87023PurposeTag S. pombe genes in C terminal with mEGFPDepositorInsertmEGFP
ExpressionBacterial and YeastAvailable SinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEB2-2ndVal mCherry2-L
Plasmid#104027PurposePlasmid encoding 2ndVal mCherry2-LDepositorInsert2ndVal mCherry2-L
UseLow copyExpressionBacterialMutationmCherry2-L with valine inserted in 2nd positionPromoterproCAvailable SinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pETNKI-strepII-3C-LIC-Amp
Plasmid#108707PurposeExpression of a StrepII-tagged target protein with 3C protease cleavage site.DepositorTypeEmpty backboneTagsStrepII-3C protease cleavage siteExpressionBacterialPromoterT7Available SinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET30b-7Y-SrtA
Plasmid#105601Purposeexpresses 7Y sortase A variant in E.coliDepositorInsertS.aureus Sortase A, 7Y mutant
TagsHis tagExpressionBacterialPromoterT7Available SinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-30
Plasmid#172754PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-30; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-30
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPBC-LG3-tdT
Plasmid#62810PurposepPBC-LG3-tdT expresses PiggyBac inverted terminal repeat-flanked Lck-GCaMP3 and tdTomato under the control of the CAG promoter.DepositorInsertITR-CAG-Lck-GCaMP3-IRES-tdTomato-ITR
ExpressionBacterial and MammalianPromoterCAGAvailable SinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTEV5_ RpMatB_1
Plasmid#78205PurposeExpression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensorDepositorInsertmatB (His6 C106A R286C Q457C K488A)
TagsHis6ExpressionBacterialMutationC106A R286C Q457C K488APromoterT7Available SinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
PclpB riboswitch GFP pZa
Plasmid#170078PurposeGFP expression under the control of E. coli clpB promoter engineered with riboregulatorsDepositorInsertGreen fluorescent protein
UseSynthetic BiologyExpressionBacterialPromoterPclpBAvailable SinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBBR5pemIKpBAD
Plasmid#129273PurposeBroad host-range plasmid with toxin-antitoxin system for maintenance in the absence of antibiotic selection, and arabinose-inducible promoterDepositorTypeEmpty backboneExpressionBacterialAvailable SinceNov. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
-
pED17x11
Plasmid#134814PurposesfGFP expression plasmid for characterizing qtRNA chargingDepositorInsertsfGFP-151-TAGA
UseSynthetic BiologyTags6xHisExpressionBacterialMutation151-TAGAAvailable SinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-28a RUVBL2 Delete
Plasmid#159143PurposeExpression and protien purification of a mutated RUVBL2DepositorInsertRUVBL2 (RUVBL2 Human)
ExpressionBacterialMutationamino acid 2-456, Thr127-Glu233 mutated to GPPGAvailable SinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAD6
Plasmid#134349PurposeE. coli mfd gene cloned into pSKB2 with an N-terminal, ppx-cleavable His6 tagDepositorAvailable SinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET22-His-TEV-DOT1L
Plasmid#89610PurposeBacterial expression vector for an N-terminally His-tagged (TEV-cleavable) version of human Dot1L residues 1-416DepositorAvailable SinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p15a-pBAD-UTR1-TetR-T500
Plasmid#45787DepositorInsertTetracycline repressor
UseSynthetic BiologyExpressionBacterialPromoterpBADAvailable SinceJuly 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
RS35075_pMQ30_KO_Kan
Plasmid#185390PurposeNon-replicating vector used to create markerless deletion of RR42_RS35075 in C. basilensis 4G11DepositorInsertsRR42_RS35075 upstream flanking homology region
RR42_RS35075 downstream flanking homology region
nptII kanamycin resistance marker
ExpressionBacterialAvailable SinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCRII-1.1-1.10a
Plasmid#69589PurposeOT-II TCR a chain cDNA in pCRIIDepositorInsertTCR alpha clone 1.1 (Tcra Mouse)
ExpressionBacterialAvailable SinceSept. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLITMUS*-PrpoN-CroAct3 63aa-PBBa_J23106-geneIII
Plasmid#134355PurposePhagemidDepositorInsertCroAct3 63aa
ExpressionBacterialAvailable SinceJune 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
ZP46 Prickle-2
Plasmid#16896DepositorInsertPrickle-2 (prickle2b Zebrafish)
ExpressionBacterialAvailable SinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBS-AMVpl-β1WT-nAchR
Plasmid#97080PurposeTo determine if structural domains are important for nicotinic acetylcholine receptor (nAChr) channel function via dose-response curves from voltage-clamped oocytes to estimate EC50's and Hill CoDepositorInsertBeta1 nicotinic acetylcholine receptor (Chrnb1 Mouse)
ExpressionBacterialMutationWTPromoterT7Available SinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits only