30,877 results

1. U_(+36GFP)-BirA

   Plasmid

   #71753

   Purpose

   U+36GFP-BirA for expression in E.Coli

   Depositor

   David Liu

   Article

   Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

   Insert

   U_(pos36GFP)_BirA_6XHis_LPETG
   

   Tags

   6XHis

   Expression

   Bacterial

   Available Since

   Dec. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDSP1

   Plasmid

   #199384

   Purpose

   Kanamycin resistant cloning vector backbone for pDSP23, pDSP31-33, pHJL1-4, pSiM1-4

   Depositor

   Hang Lu

   Type

   Empty backbone

   Expression

   Bacterial

   Mutation

   Cloning vector backbone

   Available Since

   April 20, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pCRISPR_full-site

   Plasmid

   #186277

   Purpose

   Reporter plasmid for full-site selection assay

   Depositor

   Jennifer Doudna

   Article

   Wang et al Nat Commun. 2021 May 6;12(1):2571. doi:

   Insert

   Thiomicrospira leader repeat and modified chloramphenicol selection cassette (removed RBS and start codon))
   

   Expression

   Bacterial

   Available Since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBA787

   Plasmid

   #186242

   Purpose

   soc-C HR Donor Vector (T4 phage editing)

   Depositor

   Jennifer Doudna

   Article

   Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

   Insert

   BBR1 backbone + T4 phage homology
   

   Expression

   Bacterial

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGBHPS-SortA

   Plasmid

   #21930

   Depositor

   Fuyuhiko Inagaki

   Article

   Kobashigawa et al J Biomol NMR. 2009 Mar . 43(3):145-50.

   Insert

   sortase
   

   Tags

   GB1, HRV3C cleagage site, and His

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-59

   Available Since

   Oct. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSUMO-LIC-CHIKV-nsP2h

   Plasmid

   #127935

   Purpose

   Expresses CHIKV nsP2h (1-465 aa) in bacterial cells

   Depositor

   Dahai Luo

   Article

   Law et al Proc Natl Acad Sci U S A. 2019 May 7;116

   Insert

   CHIKV nsP2h
   

   Tags

   His6-Sumo-TEV

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   July 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lapronic/pRSETB

   Plasmid

   #140755

   Purpose

   FRET-Based Genetically Encoded Nanosensor for Lactate/Pyruvate Ratio

   Depositor

   Luis Felipe Barros

   Article

   Galaz et al Anal Chem. 2020 Jun 29. doi: 10.1021/acs

   Insert

   Lapronic (Lactate/PYRuvate Ratio Optical Nano-Indicator from CECs)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMP4657

   Plasmid

   #107773

   Purpose

   Expression of EGFP

   Depositor

   Herman Spaink

   Article

   Stuurman et al Mol Plant Microbe Interact. 2000 Nov;13(

   Insert

   pLacZ-EGFP in pME6010
   

   Tags

   pLacZ-EGFP

   Expression

   Bacterial

   Promoter

   pLacZ

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pENTR FOXP3

   Plasmid

   #16363

   Depositor

   Anjana Rao

   Article

   Wu et al Cell. 2006 Jul 28. 126(2):375-87.

   Insert

   FOXP3 (FOXP3 Human)
   

   Tags

   Myc

   Expression

   Bacterial

   Available Since

   Nov. 30, 2007

   Availability

   Academic Institutions and Nonprofits only

10. CLK2

    Plasmid

    #39063

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    CLK2 (CLK2 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pShoCAST-sgRNA_entry (BO1)

    Plasmid

    #181786

    Purpose

    Expresses ShoCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    ShoTnsB, ShoTnsC, ShoTniQ, ShoCas12k
    

    Expression

    Bacterial

    Promoter

    Lac and J23119

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pShoHELIX-sgRNA_entry (BO3)

    Plasmid

    #181784

    Purpose

    Expresses ShoHELIX containing a nicking I-AniI fusion to ShoTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    nAniI-ShoTnsB, ShoTnsC, ShoTniQ, ShoCas12k
    

    Expression

    Bacterial

    Mutation

    nAniI = K227M, F80K, L232K

    Promoter

    Lac and J23119

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a-pro-sumo2

    Plasmid

    #25102

    Depositor

    Guy Salvesen

    Article

    Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

    Insert

    pro-SUMO2 (SUMO2 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pET28-a(+)-6xHis-LSSmCherry1(-13)

    Plasmid

    #219415

    Purpose

    Fluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate

    Depositor

    Allie Obermeyer

    Article

    Ahn et al JACS Au. 2024 Sep 17;4(10):3800-3812. do

    Insert

    LSSmCherry1(-13)
    

    Tags

    MGHHHHHGG

    Expression

    Bacterial

    Mutation

    K137E, K199E

    Promoter

    T7

    Available Since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. mTB3-Myc tag

    Plasmid

    #210205

    Purpose

    Type 3 phage-display vector that contains kanamycin resistance and displays the Myc-tag at the N-terminus of mature protein III.

    Depositor

    Brian Kay

    Article

    Grahn et al Methods Mol Biol. 2023;2702:191-203. doi

    Insert

    Myc-tag
    

    Expression

    Bacterial

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMB2a

    Plasmid

    #190120

    Purpose

    Level 2 MoClo plasmid for A. baumannii

    Depositor

    Veronica Godoy-Carter

    Article

    Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHBintN

    Plasmid

    #48135

    Purpose

    Shuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker neomycine for B. megaterium, integration vector

    Depositor

    Dieter Jahn

    Article

    Biedendieck et al Microb Biotechnol. 2010 Jan;3(1):24-37.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. GSG2

    Plasmid

    #38915

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    GSG2 (HASPIN Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    codon-optimized

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pET28C-tCorn

    Plasmid

    #124588

    Purpose

    Bacterial Expression of tRNA-Corn aptamer

    Depositor

    Samie Jaffrey

    Article

    Song et al Nat Chem Biol. 2017 Nov;13(11):1187-1194

    Insert

    tRNA-Corn
    

    Expression

    Bacterial

    Promoter

    lacI

    Available Since

    June 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKCiR5

    Plasmid

    #149462

    Purpose

    Broad-Host range expression vector, RK2 and pUC origin, CinR(AM) activator, PCin promoter, mRFP, Gentamicin Resistance Marker.

    Depositor

    Christopher Reisch

    Article

    Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

    Insert

    CinR(AM)-mRFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PCin

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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