33,128 results

Showing: 8581 - 8600 of 33128 results

Results per page:

Sort results by:

Ki67∆FHA-mNeonGreen-IRESpuro2

Plasmid

#183739

Purpose

Expresses Ki67∆FHA-mNeonGreen

Depositor

Daniel Gerlich

Article

Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

Insert

MKI67_lacking FHA (MKI67 Human)

Tags

mNeonGreen

Expression

Bacterial and Mammalian

Mutation

MKI67(lacking FHA)

Promoter

CMV

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pPSV39-dddAI

Plasmid

#186557

Purpose

Broad host range regulated expression of the DddA immunity determinant (DddAI)

Depositor

Joseph Mougous

Article

Gallagher et al Nat Microbiol. 2022 Jun;7(6):844-855. do

Insert

dddAI

Expression

Bacterial

Promoter

lacUV5

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET30-T7-sfGFP

Plasmid

#180757

Purpose

pET30 vector missing lacO and N-terminal His tag with sfGFP-S-tag inserted between the Bgl II and T7 terminator

Depositor

Michael Black

Article

Jew et al Front Bioeng Biotechnol. 2022 Jun 23;10:

Insert

superfolder green fluorescent protein

Tags

strep tag

Expression

Bacterial

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28b_RanT24N

Plasmid

#186271

Purpose

Expression vector of His-RanT24N in E.coli

Depositor

Masahito Ikawa

Article

Mori et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

Insert

GTP-binding nuclear protein Ran (Ran Mouse)

Tags

His tagged and His-tagged at N terminal

Expression

Bacterial

Mutation

Changed T24 into N

Promoter

T7 promoter

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
MAC-C-GFP

Plasmid

#172973

Purpose

MAC-tagged GFP expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

GFP

Expression

Bacterial and Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
VPS4B, S2C

Plasmid

#184806

Purpose

Bacterial expression of VPS4B (S2C mutation)

Depositor

James Hurley

Article

Cada et al Proc Natl Acad Sci U S A. 2022 Jul 19;11

Insert

VPS4B (VPS4B Human)

Tags

His6

Expression

Bacterial

Mutation

S2C

Promoter

T7

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pST39-Ubc13-MMS2

Plasmid

#184807

Purpose

Bacterial expression of yeast UEV-E2 complex

Depositor

James Hurley

Article

Ren et al EMBO J. 2010 Mar 17;29(6):1045-54. Epub

Insert

Ubc13, MMS2

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pETcLIC-GFP

Plasmid

#183514

Purpose

The plasmid contains LIC cassette upstream of folding reporter GFP and TEV-cleavable 10x histidine purification tag. Compatible with T7 promoter-based expression system in E. coli

Depositor

Martin Högbom

Article

Grave et al Protein Expr Purif. 2022 Oct;198:106132.

Type

Empty backbone

Tags

10x His-tag and folding reporter GFP

Expression

Bacterial

Promoter

T7 lac

Available Since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-45b-mEGFP

Plasmid

#185013

Purpose

Bacterial Expression of 6xHis Sirius-GFP Fusion Protein

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

Sirius-GFP

Expression

Bacterial

Available Since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCJH027_SC101_pLAC_LbCas12a_Kan

Plasmid

#183074

Purpose

Wild-type LbCas12a (Chen et al., 2018) in a plasmid containing a SC101 origin and lac promoter for in vivo interference assay.

Depositor

Jennifer Doudna

Article

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

LbCas12a

Expression

Bacterial

Mutation

Wild-type

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCJH002_10xHis-MBP-TEVcs-Cas12c(4)_Amp

Plasmid

#183069

Purpose

Bacterial protein expression plasmid of wild-type Cas12c_4. This is a R965H version of the Cas12c in Harrington et al., 2020.

Depositor

Jennifer Doudna

Article

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

Cas12c_4

Use

CRISPR

Tags

His10 and MBP

Expression

Bacterial

Mutation

Wild-type

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LbCas12a-gRNA_BbsI_CAM

Plasmid

#183222

Purpose

LbCas12a gRNA containing BbsI restriction recognition sites in spacer sequence for Golden Gate Assembly

Depositor

Jennifer Doudna

Article

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

LbCas12a guide RNA containing BbsI sites in spacer sequence

Use

CRISPR

Expression

Bacterial

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
L4440_BioBrick-hif-1-sgRNA-B

Plasmid

#177785

Purpose

Overexpression of sgRNAs in E. coli HT115 (targeting C. elegans hif-1 promoter)

Depositor

Michael Ristow

Article

Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

Insert

hif-1 (hif-1 Nematode)

Expression

Bacterial

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDH-EF1a-mTagBFP-T2A-TetOn3G

Plasmid

#179888

Purpose

Expresses TetOn3G transcription factor selectable by hygromycin resistance (Lentiviral vector)

Depositor

Patrick Caswell

Article

Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

Insert

TetOn3G

Use

Lentiviral

Tags

mTagBFP

Expression

Bacterial and Mammalian

Promoter

EF1a

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pgWIZ-SGCAgl

Plasmid

#131456

Purpose

Encodes Alpha Sarcoglycan 3xFLAG tagged protein (Therapeutic gene) to cure LGMD2D and luciferase enzyme for in vivo live imaging

Depositor

Michele Calos

Article

Guha et al Mol Ther Methods Clin Dev. 2019 Oct 14;1

Insert

SGCA (SGCA Human)

Tags

3xFLAG

Expression

Bacterial and Mammalian

Available Since

May 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
p35u4

Plasmid

#174786

Purpose

Constitutive expression of λCI fused via three alanines to the MS2 coat protein. Useful as RNA-DNA adapter in three-hybrid system

Depositor

Katherine Berry

Article

Wang et al RNA. 2021 Apr;27(4):513-526. doi: 10.126

Insert

MS2 coat protein

Tags

λCI

Expression

Bacterial

Mutation

V30I, A81G, positions 68-80 deleted (VATQTVGGVELP…

Promoter

Synthetic Constitutive Promoter

Available Since

May 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
R619-X67-527: His6-SARS-CoV-2 N

Plasmid

#170204

Purpose

E. coli expression of SARS-CoV-2 nucleocapsid protein

Depositor

Dominic Esposito

Insert

SARS-CoV-2 N (N SARS-CoV-2)

Expression

Bacterial

Promoter

T7

Available Since

May 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28_mouse-pro-IL1B

Plasmid

#183386

Purpose

Overexpression of catalytic domain of inflammatory caspase in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

pro-IL1B (Il1b Mouse)

Tags

His-TEV

Expression

Bacterial

Promoter

T7

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pKDsgRNA-FRT

Plasmid

#183241

Purpose

anhydrotetracycline (aTC) inducible sgRNA that targets FRT scar sites and arabinose inducible lambda Red

Depositor

Karin Schnetz

Article

Rangarajan et al Microbiology (Reading). 2022 Apr;168(4).

Insert

sgRNA-FRT

Expression

Bacterial

Promoter

P-tet

Available Since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBR1-kan-Ptet-plu-gamma-ET_rhi145

Plasmid

#180427

Purpose

Genome engineering of Agrobacterium

Depositor

Jun Fu

Article

Bian et al Appl Environ Microbiol. 2022 Mar 8;88(5)

Insert

plu-gamma-ET_rhi145

Expression

Bacterial

Available Since

April 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge