30,238 results

1. pCYP29

   Plasmid

   #212862

   Purpose

   Expresses the Streptomyces clavuligerus LRD-modifying cytochrome P450 enzyme

   Depositor

   Christopher Voigt

   Article

   Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

   Insert

   cytochrome P450
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PcymRC

   Available since

   Feb. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDM_noro_A2_lacZA

   Plasmid

   #118819

   Purpose

   T7 RNAP-driven expression of norovirus antisense orientation toehold switches (A2) with a lacZ alpha subunit reporter.

   Depositor

   Alexander Green

   Article

   Ma et al Synth Biol (Oxf). 2018;3(1):ysy018. doi:

   Insert

   Norovirus toehold switch A2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMAK462

   Plasmid

   #140700

   Purpose

   NBαMs/Rt-IgG1-Fc

   Depositor

   Takayuki Iwaki

   Article

   Iwaki et al Protein Expr Purif. 2020 Feb 13;170:1056

   Insert

   NBαMs/Rt-IgG1-Fc
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28_6xHis-PARP1delZn3

   Plasmid

   #173936

   Purpose

   Bacterial expression of PARP1 lacking Zn3 domain

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1delZn3 (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Deletion of residues 214-373; V762A

   Promoter

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOPINK-OTUD3 (OTU+UBA, aa 52-275)

   Plasmid

   #61411

   Purpose

   Expresses human OTUD3 (OTU+UBA domains) in E. coli.

   Depositor

   David Komander

   Article

   Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

   Insert

   OTUD3 (OTUD3 Human)
   

   Use

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Mutation

   Deleted aa 1-51 and aa 276-398.

   Promoter

   T7

   Available since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. His-PHD2-181-426

   Plasmid

   #223551

   Purpose

   His-tagged human PHD2 catalytic domain with TEV cleavage site between His and PHD2 for PHD2 purification

   Depositor

   Somshuvra Mukhopadhyay

   Article

   Gurol et al Proc Natl Acad Sci U S A. 2024 Jun 25;12

   Insert

   PHD2 (EGLN1 Human)
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Deleted amino acids 1-180.

   Promoter

   trc

   Available since

   Sept. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a_PA4114_Cterm_His

   Plasmid

   #220484

   Purpose

   heterologous expression of PA4114 for protein purification

   Depositor

   Emily Balskus

   Article

   Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

   Insert

   PA4114
   

   Use

   Tags

   6xHs

   Expression

   Bacterial

   Mutation

   none

   Promoter

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. U_(pos36GFP)-Cys

   Plasmid

   #71758

   Purpose

   U_+36GFP-Cys fusion expression in E.Coli

   Depositor

   David Liu

   Article

   Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

   Insert

   U_+36GFP-Cys
   

   Use

   Tags

   6XHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. HDAC6 (3GV4)

   Plasmid

   #25297

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   HDAC6_42 (HDAC6 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Contains amino acid residues 1109-1215

   Promoter

   Available since

   Aug. 16, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

10. Reb_221 aka PAS628

    Plasmid

    #71233

    Purpose

    Reb_1 with RebA(G90A)

    Depositor

    Pamela Silver

    Article

    Polka et al ACS Synth Biol. 2016 Feb 3.

    Insert

    Reb locus
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    RebA(G53A, G90A)

    Promoter

    Available since

    April 7, 2016

    Availability

    Academic Institutions and Nonprofits only

11. K560GFP_17NL

    Plasmid

    #129767

    Purpose

    Expresses Drosophila Kinesin-1 with extended neck linker (DAL insert) truncated at 560 and GFP tagged for motility assays

    Depositor

    William Hancock

    Insert

    KHC (Khc Fly)
    

    Use

    Tags

    GFP and His6

    Expression

    Bacterial

    Mutation

    DAL insert before A345

    Promoter

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX-E6AP Hect

    Plasmid

    #165102

    Purpose

    bacterial expression of a GST fusion of Human E6AP Hect domain

    Depositor

    Arthur Haas

    Article

    Ronchi et al Methods Mol Biol. 2012;832:197-218. doi:

    Insert

    E6AP Hect (UBE3A Human)
    

    Use

    Tags

    GST from plasmid.

    Expression

    Bacterial

    Mutation

    E6AP Hect domain Amino acids 495-738 (5'QLNP…

    Promoter

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. RAB3B (3DZ8)

    Plasmid

    #32879

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    RAB3B (RAB3B Human)
    

    Use

    Tags

    His tag (with TEV cleavage)

    Expression

    Bacterial

    Mutation

    amino acids 18-190 only

    Promoter

    Available since

    Jan. 11, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

14. pBADM11-HMGR

    Plasmid

    #108946

    Purpose

    heterologous expression of HMGR of S. solfataricus in E. coli

    Depositor

    Frank Schulz

    Article

    Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

    Insert

    HMG-CoA reductase (HMGR)
    

    Use

    Tags

    His6-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAB035h

    Plasmid

    #79191

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    Plac62 gIII, luxAB; Ptet rpoZ-HA4
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plac62; Ptet

    Available since

    June 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pASPIre2

    Plasmid

    #154843

    Purpose

    Derivative of pASPIre1 carrying a bxb1-sfGFP translational fusion in place of bxb1

    Depositor

    Markus Jeschek

    Article

    Hollerer et al Nat Commun. 2020 Jul 15;11(1):3551. doi:

    Insert

    Bxb1-sfGFP fusion controlled by rhamnose-inducible promoter; attB/attP-flanked discriminator containing mCherry; rhaRS operon
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. polyPRM5

    Plasmid

    #175232

    Purpose

    Bacterial expression and purification, mixed with polySH3 to form condensates

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    ABL1 (ABL1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJPC12-ΔM13-mCherry-P/PM,5C6A-GFP

    Plasmid

    #80910

    Purpose

    Reporter plasmid (mCherry and GFP)

    Depositor

    Mark Isalan

    Article

    Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

    Inserts

    mCherry
    GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P5C6A and PM,5C6A

    Available since

    Dec. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJUMP19-Pac

    Plasmid

    #126957

    Purpose

    Promoter acceptor vector. Minimal cargo vector; with eGFP without promoter in downstream secondary site. Allows introducing any part using BsmBI.

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSF1C-A-RFP

    Plasmid

    #172431

    Purpose

    StrepFlex MoClo vector for cloning in E. coli and Streptomyces expression - equivalent of pTU1-A-RFP from EcoFlex (ACS Synth. Biol. 2016, 5, 10, 1059–1069)

    Depositor

    Simon Moore, Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2021 Feb 19;10(2):402-41

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    None

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge