34,239 results
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Plasmid#214893PurposeExpresses equimolar Plasma Membrane- and Endoplasmic Reticulum- targeted split-FAST fragments for Short-range ER-PM Contact sitesDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSPLICSFAST-ER-PM-P2A
TagsnoneExpressionBacterial and MammalianAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKERepPar
Plasmid#211827PurposeMedium copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein and a RP4 partitioning system to increase segregational stability.DepositorInserteGFP
ExpressionBacterialPromoterT5Available sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUdO1a
Plasmid#210226Purposehigh-copy plasmid for bacterial expressionDepositorTypeEmpty backboneExpressionBacterialAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET23a-H6-TEV-cSrc
Plasmid#214233PurposeBacterial expression of the kinase domain of cSrc with a TEV-cleavable N-terminal 6xHis affinity tag; For in vitro tyrosine phosphorylation in peptides/proteins and for kinase specificity screeningsDepositorAvailable sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKESaPar
Plasmid#211829PurposeLow copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein and a RP4 partitioning system to increase segregational stability.DepositorInserteGFP
ExpressionBacterialPromoterT5Available sinceFeb. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
Skp
Plasmid#213139PurposeExpresses chaperone protein SKPDepositorAvailable sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCYP64
Plasmid#212897PurposeExpresses the Priestia megaterium LRD-modifying cytochrome P450 enzymeDepositorInsertcytochrome P450
ExpressionBacterialMutationP450BM3 MERO1 (V78A, A82G, F87V, P142S, T175I, A1…PromoterPcymRCAvailable sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEK39
Plasmid#210599PurposeReporter gene expression in B. dendrobatidisDepositorInsertSpH2Bpro-Hshph-G4S-HsmRuby3-ScADH1ter
TagsmRuby3ExpressionBacterial and YeastPromoterSpH2BproAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pINTO_7/8
Plasmid#206431PurposeA vector backbone (pCC1BAC-based) containing a single SapI recognition site. When digested, it exposes overhangs that allow integration of the vector into the P. tricornutum chloroplast genome.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUC19-link-sfGFP-FLAG:Kan
Plasmid#211833PurposePlasmid template that encodes the fluorescent protein sfGFP with a FLAG tag next to kanamycin resistance gene. This template was used to copy out sfGFP-FLAG-Kan for genetic tagging.DepositorInsertsfGFP-FLAG-Kan
ExpressionBacterialMutationNAAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
EV-A71 3C protease; aliases: Enterovirus A71 3C protease, non cleavable C-term 6xHis tag
Plasmid#204816PurposeBacterial expresison of Enterovirus A71 3C proteaseDepositorInsertEV A71 3C protease
ExpressionBacterialAvailable sinceJan. 17, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a-cMSPdH5
Plasmid#199771PurposeExpresses split-intein fused MSPdH5 for circularization during expression (external His-Tag)DepositorInsertcMSPdH5
TagsHis6-TagExpressionBacterialMutationexternal His-Tags flanking the C and N terminiAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTetO-ISce1-TetO
Plasmid#58506PurposeISce1 restriction site is flanked by Tet operator repeats, 96 on each sideDepositorInsertTet repeats flanking ISce1 restriction site
ExpressionBacterialPromoterno promoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSL4529 (pTnpA)
Plasmid#210869PurposeExpresses GstTnpA from pLac promoterDepositorInsertGstTnpA
ExpressionBacterialAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSL4369 (pEffector_TnpB2,ωRNA)
Plasmid#210871PurposeExpresses GstTnpB2 and native ωRNA from constitutive promotersDepositorInsertGstTnpB2
ExpressionBacterialAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSL5200 (pDonor_ISGst3)
Plasmid#210875PurposeHarbors a native ISGst3 transposon derived form Geobacillus stearothermophilus DSM 458DepositorInsertISGst3
ExpressionBacterialAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKM493
Plasmid#140191PurposeORBIT integrating plasmid for C-terminal tagging with cleavable EGFP.DepositorInsertTEV-Flag-Gly4-eGFP
TagsFlag + eGFPExpressionBacterialPromoterPGroELAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETSUMO-TtAgo
Plasmid#210191PurposeBacterial expression of the His- and SUMO-tagged Argonaute protein of Thermus thermophilusDepositorInsertTtAgo (TT_P0026 Thermus thermophilus)
Tags6x His tag and SUMO tagExpressionBacterialPromoterT7Available sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAbaMCi-GFP-BsaI_pJMP2748
Plasmid#208888PurposeAcinetobacter baumannii CRISPRi test plasmid (GFP, BsaI cloning site in gRNA cassette).DepositorInsertdCas9, sgRNA expression cassette, sfGFP
UsePir dependent origin of replicationExpressionBacterialAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCas-MmdCas12m-CBE1
Plasmid#192284PurposeMmdCas12m-CBE1 expressed under a constitutive promoterDepositorInsertMmdCas12m-CDA-UGI
UseCRISPRExpressionBacterialMutationD485APromoterPJ23108Available sinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only