30,239 results

1. pET28_6xHis-PARP1-C125G

   Plasmid

   #174792

   Purpose

   Bacterial expression of a less active PARP1 mutant (C125G reduces affinity for single-strand DNA break)

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-C125G (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   C125G, V762A

   Promoter

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRSET-B MT

   Plasmid

   #108855

   Purpose

   Encodes for the fluorescent protein mTurqoise

   Depositor

   Kalina Hristova

   Article

   Sarabipour et al Anal Biochem. 2014 Mar 15;449:155-7. doi

   Insert

   mTurqoise
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. Sperm whale Mb 64Y68F in pVP80K

   Plasmid

   #86956

   Purpose

   Increases expression of sperm whale myoglobin protein for making apomyoglobin as a reagent for measuring rates of hemin dissociation (as opposed to previous construct in pUC).

   Depositor

   John Olson

   Insert

   Mb 64Y68F
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   64Y68F

   Promoter

   T5

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTWIN1-His6-Ssp-Httex1-7Q

   Plasmid

   #84347

   Purpose

   Expression of the human Huntingtin Exon1 protein containing 7Q in E.coli

   Depositor

   Hilal Lashuel

   Article

   Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

   Insert

   Huntingtin Exon 1 (HTT Human)
   

   Use

   Tags

   N-terminal His6-tagged intein Ssp

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTriEx-PA-Rac1-C450A

   Plasmid

   #22025

   Purpose

   Expression of Rac1 fused with light-insensitive LOV2 (C450A mutation)

   Depositor

   Klaus Hahn

   Article

   Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

   Insert

   PA-Rac1-C450A (RAC1 Avena sativa (oat), Human)
   

   Use

   Tags

   6X His

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   LOV domain contains light-insensitive mutation C4…

   Promoter

   CMV, p10

   Available since

   Sept. 29, 2009

   Availability

   Academic Institutions and Nonprofits only

6. R725-X20-566: His6-MBP-tev-Avi-Hs.LGALS3

   Plasmid

   #159700

   Purpose

   E. coli protein expression

   Depositor

   Dominic Esposito

   Article

   Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

   Insert

   His6-MBP-tev-Avi-Hs.LGALS3 (LGALS3 Human)
   

   Use

   Tags

   His6-MBP-tev-Avi

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pmRFPmars

   Plasmid

   #26252

   Depositor

   Martin Fraunholz

   Article

   Paprotka et al J Microbiol Methods. 2010 Oct;83(1):82-6

   Insert

   mRFPmars
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   KpnI restriction site removed

   Promoter

   Available since

   Oct. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

8. pETx-SR6c3

   Plasmid

   #176211

   Purpose

   Bacterial expression of the SR6c3 nanobody targeting the receptor binding domain of SARS-CoV-2 spike protein

   Depositor

   Aviv Regev

   Article

   Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

   Insert

   SR6c3
   

   Use

   Tags

   6XHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAJM.714

   Plasmid

   #108515

   Purpose

   PVanCC-YFP Reporter. Van sensor for use in Marionette strain.

   Depositor

   Christopher Voigt

   Article

   Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

   Insert

   PVanCC-YFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pETM33_MAD2L1_HORMA

    Plasmid

    #178480

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    MAD2L1_HORMA (MAD2L1 Human, Synthetic)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7/LacO

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSb_init containing anti SARS-CoV-2_RBD sybody Sb#15

    Plasmid

    #153523

    Purpose

    Expression of the anti SARS-CoV-2_RBD sybody Sb#15

    Depositor

    Markus Seeger

    Article

    Walter et al bioRxiv 2020.04.16.045419

    Insert

    Sb#15
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. RAB31

    Plasmid

    #25552

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    RAB31 (RAB31 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 25, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

13. pCR2.1_HBB_N

    Plasmid

    #87847

    Purpose

    pCR2.1-based plasmid holding a 309-bp fragment containing the exon-1-exon-2 splice border of normal human β-globin mRNA

    Depositor

    Marina Kleanthous

    Article

    Patsali et al Int J Mol Sci. 2020 Sep 11;21(18). pii:

    Insert

    Homo sapiens hemoglobin subunit beta (HBB), Normal cDNA fragment (Exon1/2) (HBB Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEF073 MBP-Axin-His

    Plasmid

    #154011

    Purpose

    Expresses MBP-Axin-His (human Axin as a fusion protein with MBP and His- tags) in E.coli

    Depositor

    Jesse Zalatan

    Article

    Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

    Insert

    Axin1 (AXIN1 Human)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJExpress414_GmPOPB

    Plasmid

    #92234

    Purpose

    Express wild type prolyl oligopeptidase from Galerina marginata

    Depositor

    James Naismith

    Article

    Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

    Insert

    Prolyl oligopeptidase B from Galerina marginata
    

    Use

    Tags

    6x-Histag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    none

    Promoter

    T7 promoter

    Available since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pETM33_Nsp1

    Plasmid

    #156465

    Purpose

    Bacterial expression of Sars-CoV2 Nsp1 protein with His-tag and GST-tag

    Depositor

    Ylva Ivarsson

    Article

    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

    Insert

    Nsp1 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Gene insert is codon optimized for Ecoli.

    Promoter

    T7/LacO

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJL1 BSMT1

    Plasmid

    #106287

    Purpose

    Expresses BSMT1 in Ecoli off a T7 promoter

    Depositor

    James Collins

    Article

    Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

    Insert

    BSMT1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFC331

    Plasmid

    #87843

    Purpose

    AMA1 plasmid with Aspergillus optimized Cas9 and argB selection marker

    Depositor

    Uffe Mortensen

    Article

    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

    Inserts

    Cas9
    argB
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    Promoter

    Aspergillus nidulans tef1 promoter and native pro…

    Available since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET-His6-Sumo-MBP-CfaN

    Plasmid

    #126016

    Purpose

    Bacterial expression of MBP-CfaN fusion protein (with N-terminal His5-Sumo)

    Depositor

    Thomas Muir

    Insert

    His5-Sumo-MBP-CfaN
    

    Use

    Tags

    5xHis-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GARS_pNIC-Bio3

    Plasmid

    #153049

    Purpose

    Expression construct for producing Avi tagged Aspartyl-tRNA synthetase

    Depositor

    Susanne Gräslund

    Insert

    GARS (GARS1 Human)
    

    Use

    Tags

    Avi and His6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge