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pET3a-Ub-D77

Plasmid

#18896

Depositor

Cecile Pickart

Article

Raasi et al Methods Mol Biol. 2005;301:47-55. doi: 1

Insert

Ubiquitin-D77 (UBC Human)

Expression

Bacterial

Mutation

Aspartate residue is added to the C-terminus of t…

Available Since

Sept. 10, 2008

Availability

Academic Institutions and Nonprofits only
pET28a-pro-sumo2

Plasmid

#25102

Depositor

Guy Salvesen

Article

Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

Insert

pro-SUMO2 (SUMO2 Human)

Tags

His

Expression

Bacterial

Available Since

June 24, 2010

Availability

Academic Institutions and Nonprofits only
dCas9-CRY2Clust-FUSN-mut

Plasmid

#200970

Purpose

induction of phase-separated condensate mediated by FUSN-IDR mutant

Depositor

Jian Xu

Article

Kim et al Sci Adv. 2023 Mar 31;9(13):eadg1123. doi

Insert

FUSNmut

Use

Lentiviral

Expression

Bacterial and Mammalian

Mutation

15 Tyr-to-Ser substitutions

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pDB038b

Plasmid

#99212

Purpose

Accessory plasmid for PACE of PylRS variants

Depositor

David Liu

Article

Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

Insert

Ppsp [SD8] gIII(P29*,P83*,Y184*), luxAB; PproK pylT

Expression

Bacterial

Mutation

amber codons at positions 29, 83, and 184 of gIII

Promoter

Ppsp and PproK

Available Since

Oct. 16, 2017

Availability

Academic Institutions and Nonprofits only

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ETV1_pet28a

Plasmid

#131644

Purpose

Bacterial expression of ETS gene ETV1

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ETV1 (ETV1 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pOSV801

Plasmid

#126044

Purpose

modular integrative vector for the assembly of DNA fragments and expression in Streptomyces species

Depositor

Jean-Luc Pernodet

Article

Aubry et al Appl Environ Microbiol. 2019 Jun 7. pii:

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pAM232

Plasmid

#226654

Purpose

pET24b-p97-FLAG

Depositor

Andreas Martin

Article

Blythe et al Structure. 2019 Dec 3;27(12):1820-1829.e

Insert

p97 (VCP Human)

Tags

FLAG

Expression

Bacterial

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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E2F-1 wt-pGex2TK

Plasmid

#21668

Depositor

William Kaelin

Article

Kaelin et al Cell. 1992 Jul 24. 70(2):351-64.

Insert

E2F-1 (E2F1 Human)

Tags

GST

Expression

Bacterial

Mutation

wild type

Available Since

Sept. 4, 2009

Availability

Academic Institutions and Nonprofits only

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pET28a_LolT_C41S

Plasmid

#211789

Purpose

Expression plasmid in E. coli BL21(DE3) , which can be used for expression and purification to get protein LolT_C41S

Depositor

Yang Hai

Article

Gao et al Chembiochem. 2023 Oct 1:e202300561. doi:

Insert

lolt mutant

Tags

His tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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drebrin-His

Plasmid

#40363

Depositor

Phillip Gordon-Weeks

Article

Geraldo et al Nat Cell Biol. 2008 Oct;10(10):1181-9. E

Insert

drebrin E2 (DBN1 Human)

Tags

His and T7 tag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 19, 2012

Availability

Academic Institutions and Nonprofits only
pelB-MBP-P66-ss

Plasmid

#137045

Purpose

E. coli expression clone (T7lac promoter) for mature P66 (aa 22-618) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage

Depositor

Debra Hansen

Article

Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

Insert

AAC66949.1 (p66 Borrelia burgdorferi B31)

Tags

PelB signal peptide + His10 + maltose binding pro…

Expression

Bacterial

Mutation

mature P66: lacks the P66 signal peptide

Promoter

T7lac

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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eGFP-CBP

Plasmid

#169211

Purpose

Expression of eGFP-CBP protein for chitin visualization

Depositor

James S Fraser

Article

Ricardo-Gonzalez et al bioRxiv 2021.03.29.437438

Insert

Chitinase A1 Chitin Binding Domain

Tags

6xHis, Mxe Gyr Intein, and eGFP

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pTL2-IncB-GFP11x7

Plasmid

#113031

Purpose

Expresses IncB tagged with 7 copies of GFP11 under a tetracycline inducible promoter

Depositor

Kevin Hybiske

Article

Wang et al Pathog Dis. 2018 Mar 1;76(2). pii: 48301

Insert

IncB (incB Chlamydia trachomatis)

Tags

GFP11x7 and flag

Expression

Bacterial

Promoter

tet inducible

Available Since

Oct. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_DVLP1_DEP

Plasmid

#109806

Purpose

Protein expression and purification of DVLP1_DEP

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

DVLP1_DEP

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-41

Plasmid

#172757

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-41; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-41

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pLP-pTac-Tn5-chlorR

Plasmid

#199994

Purpose

Landing Pad with T7 RNAP circuit variant T15. No seed promoter. pTac-Tn5 transposase. In dap auxotrophic conjugation donor. This material contains 2 plasmids. Look below for more information.

Depositor

Farren Isaacs

Article

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

Landing Pads

Expression

Bacterial

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pPath-pTac-integrase

Plasmid

#199999

Purpose

Entry vector for biosynthetic pathway. pTac-phiC31 integrase. This material contains 2 plasmids. For more information look under the "Depositor Comments" section below.

Depositor

Farren Isaacs

Article

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

Pathways

Expression

Bacterial

Available Since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pQlinkH-PTPIP51(36-235)

Plasmid

#170533

Purpose

Expresses Cleavable His-tagged PTPIP51_ΔTPR domain (36-235) in E. Coli

Depositor

Byung Il Lee

Article

Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

Insert

Protein tyrosine phosphatase interacting protein 51 (RMDN3 Human)

Tags

His tag

Expression

Bacterial

Promoter

T5 promoter

Available Since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pTJV1ScKm-rpoB

Plasmid

#166980

Purpose

Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; npt2 for kanamycin resistance

Depositor

Christopher Reisch

Article

Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

Insert

NeoR/KanR

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-2T-RNAPII-CTD wt #14-HA

Plasmid

#160688

Purpose

Expression of GST-RNA Polymerase II C-terminal domain fusion protein. Contains 14 of the consensus heptapeptide repeats shown to be phosphorylated by Cdc2 kinase.

Depositor

Jeffry Corden

Article

Patturajan et al J Biol Chem. 1998 Feb 20;273(8):4689-94.

Insert

RNA Polymerase II CTD

Expression

Bacterial

Mutation

C terminal domain only

Available Since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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