30,880 results

1. pTn7xKS

   Plasmid

   #117393

   Purpose

   Tn7 tagging vector with a LacI-controlled kill switch counterselection system

   Depositor

   Karen Guillemin

   Article

   Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT7-HISx6-SIMx6

   Plasmid

   #116938

   Purpose

   Bacterial expression vector for 6 sim

   Depositor

   Liam Holt

   Article

   Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

   Insert

   6 sim
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT7-HISx6-SUMOx10

   Plasmid

   #116937

   Purpose

   Bacterial expression vector for 10 sumo

   Depositor

   Liam Holt

   Article

   Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

   Insert

   10 SUMO
   

   Tags

   HIS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. E2F-1 wt-pGex2TK

   Plasmid

   #21668

   Depositor

   William Kaelin

   Article

   Kaelin et al Cell. 1992 Jul 24. 70(2):351-64.

   Insert

   E2F-1 (E2F1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   wild type

   Available Since

   Sept. 4, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCoofy48

   Plasmid

   #55186

   Purpose

   Bacterial expression vector for parallel SLIC cloning containing N-terminal 6x His and GST tags. C-terminal tags can be added during SLIC.

   Depositor

   Sabine Suppmann

   Article

   Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

   Type

   Empty backbone

   Tags

   6x His, GST, His10-Stop-OneStrep-Stop-S-tag-Stop-…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYC1876

   Plasmid

   #158722

   Purpose

   Helper plasmid pYC1640 for C-terminal tagging with degradation tag ssrA

   Depositor

   Yi-Cheng Sun

   Article

   Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

   Insert

   ssrA
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. RP1B-PP1alpha

   Plasmid

   #51768

   Purpose

   Expresses PP1 alpha residues 7-330

   Depositor

   Wolfgang Peti

   Article

   Kelker et al J Mol Biol. 2009 Jan 9. 385(1):11-21.

   Insert

   pp1 alpha
   

   Tags

   His, TEV protease site, and Thio-6

   Expression

   Bacterial

   Mutation

   Contains PP1 alpha residues 7-330

   Promoter

   T7-lac

   Available Since

   March 25, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pAWP78-PVCpnf_pvc13-Ad5DL491-492

   Plasmid

   #198290

   Purpose

   Produces PVCs retargeted with non-binding mutant of Ad5 knob

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   PVCpnf_pvc13-Ad5DL491-492
   

   Expression

   Bacterial

   Available Since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. 3S8P

   Plasmid

   #51360

   Depositor

   Cheryl Arrowsmith

   Insert

   3S8P
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Truncation: See Comments

   Promoter

   T7

   Available Since

   Feb. 12, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

10. pCJH027_SC101_pLAC_LbCas12a_Kan

    Plasmid

    #183074

    Purpose

    Wild-type LbCas12a (Chen et al., 2018) in a plasmid containing a SC101 origin and lac promoter for in vivo interference assay.

    Depositor

    Jennifer Doudna

    Article

    Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

    Insert

    LbCas12a
    

    Expression

    Bacterial

    Mutation

    Wild-type

    Available Since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET21c(+)-mtPheRS

    Plasmid

    #31080

    Depositor

    Linda Spremulli

    Article

    Bullard et al J Mol Biol. 1999 May 14. 288(4):567-77.

    Insert

    Mature form human mitochondrial phenylalanine-tRNA synthetase (FARS2 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Includes coding sequence for the mature form. Nt …

    Promoter

    T7

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

12. Sc eRF3∆165N (WT)

    Plasmid

    #175418

    Purpose

    Encodes His-MBP-TEV-WT eRF3∆165N

    Depositor

    Joseph Puglisi

    Article

    Lawson et al Science. 2021 Aug 20;373(6557):876-882.

    Insert

    eRF3 (SUP35 Budding Yeast)
    

    Tags

    His-MBP-TEV

    Expression

    Bacterial

    Mutation

    ∆165N

    Promoter

    T7

    Available Since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRSFDuet-1_VNp-hGH

    Plasmid

    #182411

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-human growth hormone fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp-hGH (GH1 Human)
    

    Tags

    Vesicle Nucleating peptide (VNp)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pL0M-S-tdTomato-EC18158

    Plasmid

    #137078

    Purpose

    Golden Gate Level 0 S module for N-terminal fluorescent protein tagging

    Depositor

    Julian Hibberd

    Article

    Luginbuehl et al Plant Direct. 2020 Feb 11;4(2):e00188. d

    Insert

    tdTomato DNA synthesis product with 5' and 3' extensions for Golden Gate cloning
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    June 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pZSm45 ndhII

    Plasmid

    #48889

    Purpose

    Lux-regulated expression of NDH-2 enzyme

    Depositor

    Jeff Hasty

    Article

    Prindle et al Nature. 2011 Dec 18;481(7379):39-44. doi

    Insert

    ndhII and LuxR
    

    Use

    Synthetic Biology

    Tags

    ssrA tag (AANDENYALAA)

    Expression

    Bacterial

    Promoter

    pLux

    Available Since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRS413-GAL1-luc*(-SKL)

    Plasmid

    #40234

    Depositor

    Michael Benton

    Insert

    luc*(-SKL)
    

    Expression

    Bacterial

    Promoter

    GAL1

    Available Since

    Oct. 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET3a-Ub-D77

    Plasmid

    #18896

    Depositor

    Cecile Pickart

    Article

    Raasi et al Methods Mol Biol. 2005;301:47-55. doi: 1

    Insert

    Ubiquitin-D77 (UBC Human)
    

    Expression

    Bacterial

    Mutation

    Aspartate residue is added to the C-terminus of t…

    Available Since

    Sept. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

18. pTriEx-mCherry-LOVSD

    Plasmid

    #81033

    Purpose

    Mammalian expression of the super-dark state of LOV2

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    LOVSD
    

    Tags

    mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    C450A, L514K, G528A, L531E, N538E

    Available Since

    Aug. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRSFDuetG-HsNot1iso2(MCSI)_O

    Plasmid

    #147088

    Purpose

    Bacterial Expression of HsNot1iso2

    Depositor

    Elisa Izaurralde

    Insert

    HsNot1iso2 (CNOT1 Human)
    

    Expression

    Bacterial

    Available Since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRSET FLIPglu-600uDelta13

    Plasmid

    #13559

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

    Insert

    FLIPglu F16A Delta13
    

    Tags

    ECFP and EYFP

    Expression

    Bacterial

    Mutation

    F16A in binding protein

    Available Since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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